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AB254722

Anti-NUP54 antibody

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(1 Publication)

Rabbit Polyclonal NUP54 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Nucleoporin p54 aa 250-350.

View Alternative Names

Nucleoporin p54, 54 kDa nucleoporin, NUP54

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP54 antibody (AB254722)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP54 antibody (AB254722)

Paraffin-embedded human stomach tissue stained for NUP54 using ab254722 at 1/20 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP54 antibody (AB254722)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP54 antibody (AB254722)

Paraffin-embedded human skeletal muscle tissue stained for NUP54 using ab254722 at 1/20 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP54 antibody (AB254722)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP54 antibody (AB254722)

Paraffin-embedded human placenta tissue stained for NUP54 using ab254722 at 1/20 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP54 antibody (AB254722)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP54 antibody (AB254722)

Paraffin-embedded human testis tissue stained for NUP54 using ab254722 at 1/20 dilution in immunohistochemical analysis.

Western blot - Anti-NUP54 antibody (AB254722)
  • WB

Supplier Data

Western blot - Anti-NUP54 antibody (AB254722)

Lane 1:

Western blot - Anti-NUP54 antibody (ab254722)

Lane 2:

Western blot - Anti-NUP54 antibody (ab254722) at 0.4 µg/mL

Lane 1:

MW Ladder

Lane 2:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Predicted band size: 55 kDa

false

Western blot - Anti-NUP54 antibody (AB254722)
  • WB

Supplier Data

Western blot - Anti-NUP54 antibody (AB254722)

Lane 1:

Western blot - Anti-NUP54 antibody (ab254722)

Lanes 2 - 3:

Western blot - Anti-NUP54 antibody (ab254722) at 0.4 µg/mL

Lane 2:

NIH-3T3 whole cell lysate

Lane 3:

NBT-II whole cell lysate

Predicted band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Nucleoporin p54 aa 250-350. The exact immunogen used to generate this antibody is proprietary information.

Q7Z3B4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NUP54 also known as Nuclear Pore Complex Protein Nup54 or nucleoporin 54kDa functions as a part of the nuclear pore complex (NPC) a structure facilitating the transport of molecules across the nuclear envelope. This protein has a molecular mass of approximately 54 kDa and is ubiquitously expressed in eukaryotic cells. NUP54 is integral to the assembly and stability of the NPC and it assists in the regulation of nucleocytoplasmic transport by acting as a structural component within the pore.
Biological function summary

NUP54 plays an important role in the movement of proteins and RNA due to its position within the nuclear pore complex. Acting as a pivotal component of the NPC NUP54 interacts with other nucleoporins to form a selective barrier that controls the bidirectional movement of molecules. Proteins such as NUP62 and NUP58 are also part of this complex working alongside NUP54 to ensure efficient transport processes necessary for cell function and regulation.

Pathways

NUP54 is significantly involved in the protein and mRNA export pathways essential steps for cellular communication and regulation. It ensures the proper translocation of molecules through the nuclear pore working closely with other proteins such as importins and exportins within these pathways. NUP54's involvement in these pathways highlights its importance in maintaining cellular homeostasis and facilitating signal transduction from the nucleus to the cytoplasm.

NUP54 has connections to specific conditions such as neurodegenerative diseases and cancer. Mutations or dysregulation in NUP54 can lead to altered nucleocytoplasmic transport potentially contributing to the pathogenesis of neurodegenerative diseases. In cancer NUP54 may influence tumor progression through its interactions with other nucleoporins like NUP214 which is frequently overexpressed or rearranged in various tumor types. Understanding NUP54's role in these diseases could provide insights for therapeutic targeting.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the nuclear pore complex, a complex required for the trafficking across the nuclear membrane.
See full target information Nucleoporin p54
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of mammary gland biology and neoplasia 28:3 PubMed36801983

2023

Valine Treatment Enhances Antimicrobial Component Production in Mammary Epithelial Cells and the Milk of Lactating Goats Without Influencing the Tight Junction Barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Yusaku Tsugami,Takahiro Nii,Naoki Isobe
View all publications
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Product promise

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