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AB238735

Anti-NUP93 antibody

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(1 Publication)

Rabbit Polyclonal NUP93 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NUP93 aa 1-200.

View Alternative Names

KIAA0095, NUP93, Nuclear pore complex protein Nup93, 93 kDa nucleoporin, Nucleoporin Nup93

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP93 antibody (AB238735)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP93 antibody (AB238735)

Paraffin-embedded human testis tissue stained for NUP93 using ab238735 at 1/400 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human NUP93 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q8N1F7

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Alternative Products

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Secondary Antibodies

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Primary Antibodies

AB140651

Anti-Nucleoporin p62/NUP62 antibody [EPR9919]

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Western blot - Anti-Nucleoporin p62/NUP62 antibody [EPR9919] (AB140651)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity greater than 95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NUP93 also known as nucleoporin 93 is a protein with a molecular mass of approximately 93 kDa. It forms a part of the nuclear pore complex (NPC) specifically contributing to its structural integrity. NUP93 can be found in the nuclear envelope of eukaryotic cells where it aids in the proper functioning of the NPC. The protein ensures that macromolecules can efficiently transport between the nucleus and the cytoplasm therefore maintaining cellular function.
Biological function summary

NUP93 plays an important role in the assembly and stability of the nuclear pore complex by interacting with other nucleoporins. It is a component of the core scaffold structure interacting with proteins like NUP205 and NUP188 and helps in the spatial arrangement and functionality within the complex. Through these interactions NUP93 contributes to selective transport acting as gatekeeper to regulate traffic into and out of the nucleus.

Pathways

NUP93 influences various cellular processes by affecting nuclear transport pathways such as the nucleocytoplasmic transport pathway. Through its connectivity in the nuclear pore complex it affects the transit of molecules like messenger RNA (mRNA) and ribonucleoprotein complexes. NUP93 is associated with proteins such as importin-beta which is involved in the import of proteins with nuclear localization signals thereby ensuring precise nuclear-cytoplasmic communication.

Mutations or dysregulation of NUP93 have associations with nephrotic syndrome and some forms of cancer. Nephrotic syndrome can result from altered nuclear pore function where NUP93 links with proteins like podocin affecting kidney filtration efficiency. In cancer changes in nuclear transport potentially involving interactions with oncogenic proteins can disrupt cell cycle regulation and contribute to tumorogenic processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance (PubMed : 9348540). May anchor nucleoporins, but not NUP153 and TPR, to the NPC. During renal development, regulates podocyte migration and proliferation through SMAD4 signaling (PubMed : 26878725).
See full target information NUP93
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Bioengineered 12:5323-5333 PubMed34499008

2021

The tumor-suppressive effects of alpha-ketoglutarate-dependent dioxygenase FTO via N6-methyladenosine RNA methylation on bladder cancer patients.

Applications

Unspecified application

Species

Unspecified reactive species

Wenfa Yi,Yong Yu,Yafei Li,Juan Yang,Shuying Gao,Lingfen Xu
View all publications
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Product promise

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