Anti-NUP98 antibody [13C2 + 21A10] - BSA and Azide free (ab179911)

Mouse Multiclonal NUP98 antibody. Suitable for WB, ICC/IF and reacts with Schizosaccharomyces pombe, Saccharomyces cerevisiae, Tetrahymena, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human NUP98 aa 1-50.

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Images

Western blot - Anti-NUP98 antibody [13C2 + 21A10] - BSA and Azide free (AB179911), expandable thumbnail

Publications

Key facts

Isotype: IgG1
Host species: Mouse
Storage buffer: pH: 6 - 8.5
Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS
Form: Liquid
Clonality: Multiclonal

Immunogen

Synthetic Peptide within Human NUP98 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P52948

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	ICC/IF
Human	Tested	Tested
Saccharomyces cerevisiae	Tested	Tested
Schizosaccharomyces pombe	Tested	Tested
Tetrahymena	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Schizosaccharomyces pombe	Dilution info 0.40000-2.00000 µg/mL	Notes Clone 13C2 antibody is most suitable for this application in Human, Tetrahymena, S. cerevisiae and S. pombe.
Species Saccharomyces cerevisiae	Dilution info 0.40000-2.00000 µg/mL	Notes Clone 13C2 antibody is most suitable for this application in Human, Tetrahymena, S. cerevisiae and S. pombe.
Species Tetrahymena	Dilution info 0.40000-2.00000 µg/mL	Notes Clone 13C2 antibody is most suitable for this application in Human, Tetrahymena, S. cerevisiae and S. pombe.
Species Human	Dilution info 0.40000-2.00000 µg/mL	Notes Clone 13C2 antibody is most suitable for this application in Human, Tetrahymena, S. cerevisiae and S. pombe.

Tested
Tested

Species	Dilution info	Notes
Species Schizosaccharomyces pombe	Dilution info 0.50000-10.00000 µg/mL	Notes Clone 21A10 antibody is most suitable for this application in Human, Tetrahymena, S. cerevisiae and S. pombe.
Species Saccharomyces cerevisiae	Dilution info 0.50000-10.00000 µg/mL	Notes Clone 21A10 antibody is most suitable for this application in Human, Tetrahymena, S. cerevisiae and S. pombe.
Species Tetrahymena	Dilution info 0.50000-10.00000 µg/mL	Notes Clone 21A10 antibody is most suitable for this application in Human, Tetrahymena, S. cerevisiae and S. pombe.
Species Human	Dilution info 0.50000-10.00000 µg/mL	Notes Clone 21A10 antibody is most suitable for this application in Human, Tetrahymena, S. cerevisiae and S. pombe.

Associated Products

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Target data

See full target information NUP98

Function

Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. NUP98 and NUP96 are involved in the bidirectional transport across the NPC (PubMed:33097660). May anchor NUP153 and TPR to the NPC. In cooperation with DHX9, plays a role in transcription and alternative splicing activation of a subset of genes (PubMed:28221134). Involved in the localization of DHX9 in discrete intranuclear foci (GLFG-body) (PubMed:28221134). (Microbial infection) Interacts with HIV-1 capsid protein P24 and nucleocapsid protein P7 and may thereby promote the integration of the virus in the host nucleus (in vitro) (PubMed:23523133). Binding affinity to HIV-1 CA-NC complexes bearing the capsid change Asn-74-Asp is reduced (in vitro) (PubMed:23523133).

Alternative names

ADAR2, NUP98, Nuclear pore complex protein Nup98-Nup96

Key facts

Isotype: IgG1
Host species: Mouse
Storage buffer: pH: 6 - 8.5
Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS
Form: Liquid
Clonality: Multiclonal
Immunogens: Synthetic Peptide within Human NUP98 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P52948
Clone number: 13C2 + 21A10
Purification technique: Affinity purification Protein G
Specificity: ab179911 crossreacts with multiple nucleoproteins of S. cerevisiae, e.g. Nup116, Nup100, Nup145N, Nup57 and Nup9.
Epitope: FGxxN for clone 13C2 and GLF for clone 21A10.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is an oligoclonal antibody?
This oligoclonal antibody is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The NUP98 protein also known as nucleoporin 98 plays an important role in cellular mechanics. It forms a part of the nuclear pore complex important for regulating nucleocytoplasmic transport. With an approximate mass of 98 kDa NUP98 expresses mainly in the nucleus across various cell types. Alternative splicing of the NUP98 gene can lead to different isoforms. In some research contexts NUP98 is also referred to by related terms such as NUP96. Its expression appears widespread in many tissues replacing or complementing other nucleoporins.

Biological function summary

The NUP98 protein contributes to nuclear-cytoplasmic transport processes as part of the nuclear pore complex. In association with other nucleoporins NUP98 acts in cargo transport between the nucleus and cytoplasm facilitating the movement of RNA and proteins. It also participates in the assembly and disassembly cycles of the nuclear pore complex (NPC). Known interactions include those with components like the 13C2 complex and other mRNA export factors. NUP98's function impacts NPC architecture and its transport efficiency.

Pathways

NUP98 has essential roles beyond mechanical transport. It integrates into the mRNA export pathway collaborating with proteins like CRM1 (exportin-1) to regulate mRNA export. Additionally it plays a role in gene expression regulation by binding to gene promoters involving pathways linked to protein synthesis and cell cycle regulation. Connections with histone modifications suggest its involvement in chromatin remodeling linking it further to the regulation of gene expression pathways.

Associated diseases and disorders

NUP98 associations are significant. It is implicated in certain hematological malignancies like acute myeloid leukemia (AML) where it forms fusion proteins with partners such as HOXA9 and NSD1. Genetic rearrangements involving NUP98 contribute to oncogenesis altering normal cell function. Other conditions including dysregulation in autoimmune diseases suggest potential links to aberrant signaling pathways. Understanding these disease links involves studying interactions with proteins like MLL another significant player in leukemia pathogenesis.

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9 product images

Western blot - Anti-NUP98 antibody [13C2 + 21A10] - BSA and Azide free (ab179911)
All lanes: Western blot - Anti-NUP98 antibody [13C2 + 21A10] - BSA and Azide free (ab179911) at 0.4 µg/mL
All lanes: HeLa cell extract
Secondary
All lanes: HRP-labeled anti-mouse IgG at 0.4 µg/mL
Developed using the ECL technique.
Predicted band size: 198 kDa
Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2 + 21A10] - BSA and Azide free (ab179911)
Immunofluorescence analysis of methanol-fixed HeLa cells, labeling NUP98 using two different clones of ab179911 at 0.5 μg/ml, followed by Alexa Fluor 488-conjugated anti-mouse lgG (green) at 4 μg/ml. DAPI was used to stain DNA (magenta). Upper and middle panels correspond to black-and-white images while the bottom panel represents merged colored images.
Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2 + 21A10] - BSA and Azide free (ab179911)
Immunofluorescence analysis of methanol-fixed Tetrahymena thermophila cells, labeling NUP98 using two different clones of ab179911 at 0.5 μg/ml, followed by Alexa Fluor 488-conjugated anti-mouse lgG (green) at 4 μg/ml. DAPI was used to stain DNA (magenta). Upper and middle panels correspond to black-and-white images while the bottom panel represents merged images. Dotted lines represent the outlines of cells. The open arrow indicates the micronucleus. Insets are magnified images showing the position of the micronucleus.
Western blot - Anti-NUP98 antibody [13C2 + 21A10] - BSA and Azide free (ab179911)
Open arrowheads, wild type NUP98. Solid arrow, NUP98 fused to a fluorescence protein. Diamonds and asterisks represent uncharacterized proteins. For lane 3 exposure time was about 10 times longer than for the other lanes.
All lanes: Western blot - Anti-NUP98 antibody [13C2 + 21A10] - BSA and Azide free (ab179911) at 2 µg/mL
Lanes 1 - 3: Tetrahymena thermophila cell extract
Lane 4: Cell extract from Tetrahymena thermophila expressing endogenously NUP98 fused to a fluorescence protein
Developed using the ECL technique.
Predicted band size: 198 kDa
Observed band size: 125 kDa, 98 kDa
Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2 + 21A10] - BSA and Azide free (ab179911)
Immunofluorescence analysis of formaldehyde-fixed, zymolyase-treated, S. pombe cells, labeling NUP98 using two different clones of ab179911 at 10 μg/ml, followed by Alexa Fluor 488-conjugated anti-mouse lgG (green). DAPI was used to stain DNA (magenta). Upper and middle panels correspond to black-and-white images while the bottom panel represents merged images. Dotted lines represent the outlines of cells.
Western blot - Anti-NUP98 antibody [13C2 + 21A10] - BSA and Azide free (ab179911)
All lanes: Western blot - Anti-NUP98 antibody [13C2 + 21A10] - BSA and Azide free (ab179911) at 2 µg/mL
Lanes 1 and 3: Cell extract from S. pombe wild type
Lanes 2 and 4: Cell extract from S. pombe expressing endogenously NUP98 fused to a fluorescence protein
Developed using the ECL technique.
Predicted band size: 198 kDa
Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2 + 21A10] - BSA and Azide free (ab179911)
Immunofluorescence analysis of formaldehyde-fixed, zymolyase-treated, S. cerevisiae cells, labeling NUP98 using two different clones of ab179911 at 10 μg/ml, followed by Alexa Fluor 488-conjugated anti-mouse lgG (green). DAPI was used to stain DNA (magenta). Upper and middle panels correspond to black-and-white images while the bottom panel represents merged images. Dotted lines represent the outlines of cells.
Western blot - Anti-NUP98 antibody [13C2 + 21A10] - BSA and Azide free (ab179911)
Asterisks represent uncharacterized proteins.
All lanes: Western blot - Anti-NUP98 antibody [13C2 + 21A10] - BSA and Azide free (ab179911) at 2 µg/mL
All lanes: S. cerevisiae cell extract
Developed using the ECL technique.
Predicted band size: 198 kDa
Functional Studies - Anti-NUP98 antibody [13C2 + 21A10] - BSA and Azide free (ab179911)
Summary of the suitability of ab179911 clones for immunological applications. IF: indirect immunofluorescence staining; WB: Western blotting analysis.