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AB179911

Anti-NUP98 antibody [13C2 + 21A10]

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(2 Publications)

Mouse Multiclonal NUP98 antibody. Suitable for WB, ICC/IF and reacts with Schizosaccharomyces pombe, Saccharomyces cerevisiae, Tetrahymena, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human NUP98 aa 1-50.

View Alternative Names

ADAR2, NUP98, Nuclear pore complex protein Nup98-Nup96

9 Images
Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2 + 21A10] (AB179911)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2 + 21A10] (AB179911)

Immunofluorescence analysis of methanol-fixed HeLa cells, labeling NUP98 using two different clones of ab179911 at 0.5 μg/ml, followed by Alexa Fluor 488-conjugated anti-mouse lgG (green) at 4 μg/ml. DAPI was used to stain DNA (magenta). Upper and middle panels correspond to black-and-white images while the bottom panel represents merged colored images.

Western blot - Anti-NUP98 antibody [13C2 + 21A10] (AB179911)
  • WB

Supplier Data

Western blot - Anti-NUP98 antibody [13C2 + 21A10] (AB179911)

All lanes:

Western blot - Anti-NUP98 antibody [13C2 + 21A10] (ab179911) at 0.4 µg/mL

All lanes:

HeLa cell extract

Secondary

All lanes:

HRP-labeled anti-mouse IgG at 0.4 µg/mL

Predicted band size: 198 kDa

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Functional Studies - Anti-NUP98 antibody [13C2 + 21A10] (AB179911)
  • FuncS

Supplier Data

Functional Studies - Anti-NUP98 antibody [13C2 + 21A10] (AB179911)

Summary of the suitability of ab179911 clones for immunological applications. IF : indirect immunofluorescence staining; WB : Western blotting analysis.

Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2 + 21A10] (AB179911)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2 + 21A10] (AB179911)

Immunofluorescence analysis of formaldehyde-fixed, zymolyase-treated, S. pombe cells, labeling NUP98 using two different clones of ab179911 at 10 μg/ml, followed by Alexa Fluor 488-conjugated anti-mouse lgG (green). DAPI was used to stain DNA (magenta). Upper and middle panels correspond to black-and-white images while the bottom panel represents merged images. Dotted lines represent the outlines of cells.

Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2 + 21A10] (AB179911)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2 + 21A10] (AB179911)

Immunofluorescence analysis of methanol-fixed Tetrahymena thermophila cells, labeling NUP98 using two different clones of ab179911 at 0.5 μg/ml, followed by Alexa Fluor 488-conjugated anti-mouse lgG (green) at 4 μg/ml. DAPI was used to stain DNA (magenta). Upper and middle panels correspond to black-and-white images while the bottom panel represents merged images. Dotted lines represent the outlines of cells. The open arrow indicates the micronucleus. Insets are magnified images showing the position of the micronucleus.

Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2 + 21A10] (AB179911)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2 + 21A10] (AB179911)

Immunofluorescence analysis of formaldehyde-fixed, zymolyase-treated, S. cerevisiae cells, labeling NUP98 using two different clones of ab179911 at 10 μg/ml, followed by Alexa Fluor 488-conjugated anti-mouse lgG (green). DAPI was used to stain DNA (magenta). Upper and middle panels correspond to black-and-white images while the bottom panel represents merged images. Dotted lines represent the outlines of cells.

Western blot - Anti-NUP98 antibody [13C2 + 21A10] (AB179911)
  • WB

Supplier Data

Western blot - Anti-NUP98 antibody [13C2 + 21A10] (AB179911)

Asterisks represent uncharacterized proteins.

All lanes:

Western blot - Anti-NUP98 antibody [13C2 + 21A10] (ab179911) at 2 µg/mL

All lanes:

S. cerevisiae cell extract

Predicted band size: 198 kDa

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Western blot - Anti-NUP98 antibody [13C2 + 21A10] (AB179911)
  • WB

Supplier Data

Western blot - Anti-NUP98 antibody [13C2 + 21A10] (AB179911)

Open arrowheads, wild type NUP98. Solid arrow, NUP98 fused to a fluorescence protein. Diamonds and asterisks represent uncharacterized proteins. For lane 3 exposure time was about 10 times longer than for the other lanes.

All lanes:

Western blot - Anti-NUP98 antibody [13C2 + 21A10] (ab179911) at 2 µg/mL

Lanes 1 - 3:

Tetrahymena thermophila cell extract

Lane 4:

Cell extract from Tetrahymena thermophila expressing endogenously NUP98 fused to a fluorescence protein

Predicted band size: 198 kDa

Observed band size: 125 kDa,98 kDa

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Western blot - Anti-NUP98 antibody [13C2 + 21A10] (AB179911)
  • WB

Supplier Data

Western blot - Anti-NUP98 antibody [13C2 + 21A10] (AB179911)

All lanes:

Western blot - Anti-NUP98 antibody [13C2 + 21A10] (ab179911) at 2 µg/mL

Lanes 1 and 3:

Cell extract from S. pombe wild type

Lanes 2 and 4:

Cell extract from S. pombe expressing endogenously NUP98 fused to a fluorescence protein

Predicted band size: 198 kDa

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Key facts

Host species

Mouse

Clonality

Multiclonal

Clone number

13C2 + 21A10

Isotype

IgG1

Carrier free

No

Reacts with

Human, Saccharomyces cerevisiae, Tetrahymena, Schizosaccharomyces pombe

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Synthetic Peptide within Human NUP98 aa 600-700. Database link P52948

Epitope

FGxxN for clone 13C2 and GLF for clone 21A10.

Specificity

ab179911 crossreacts with multiple nucleoproteins of S. cerevisiae, e.g. Nup116, Nup100, Nup145N, Nup57 and Nup9.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.4-2 µg/mL", "WB-species-notes": "<p>Clone 13C2 antibody is most suitable for this application in Human, Tetrahymena, S. cerevisiae and S. pombe.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.5-10 µg/mL", "ICCIF-species-notes": "<p>Clone 21A10 antibody is most suitable for this application in Human, Tetrahymena, S. cerevisiae and S. pombe.</p>" }, "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.4-2 µg/mL", "WB-species-notes": "<p>Clone 13C2 antibody is most suitable for this application in Human, Tetrahymena, S. cerevisiae and S. pombe.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.5-10 µg/mL", "ICCIF-species-notes": "<p>Clone 21A10 antibody is most suitable for this application in Human, Tetrahymena, S. cerevisiae and S. pombe.</p>" }, "Schizosaccharomyces pombe": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.4-2 µg/mL", "WB-species-notes": "<p>Clone 13C2 antibody is most suitable for this application in Human, Tetrahymena, S. cerevisiae and S. pombe.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.5-10 µg/mL", "ICCIF-species-notes": "<p>Clone 21A10 antibody is most suitable for this application in Human, Tetrahymena, S. cerevisiae and S. pombe.</p>" }, "Tetrahymena": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.4-2 µg/mL", "WB-species-notes": "<p>Clone 13C2 antibody is most suitable for this application in Human, Tetrahymena, S. cerevisiae and S. pombe.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.5-10 µg/mL", "ICCIF-species-notes": "<p>Clone 21A10 antibody is most suitable for this application in Human, Tetrahymena, S. cerevisiae and S. pombe.</p>" } } }
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Product details

What is an oligoclonal antibody?
This oligoclonal antibody is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 6 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NUP98 protein also known as nucleoporin 98 plays an important role in cellular mechanics. It forms a part of the nuclear pore complex important for regulating nucleocytoplasmic transport. With an approximate mass of 98 kDa NUP98 expresses mainly in the nucleus across various cell types. Alternative splicing of the NUP98 gene can lead to different isoforms. In some research contexts NUP98 is also referred to by related terms such as NUP96. Its expression appears widespread in many tissues replacing or complementing other nucleoporins.
Biological function summary

The NUP98 protein contributes to nuclear-cytoplasmic transport processes as part of the nuclear pore complex. In association with other nucleoporins NUP98 acts in cargo transport between the nucleus and cytoplasm facilitating the movement of RNA and proteins. It also participates in the assembly and disassembly cycles of the nuclear pore complex (NPC). Known interactions include those with components like the 13C2 complex and other mRNA export factors. NUP98's function impacts NPC architecture and its transport efficiency.

Pathways

NUP98 has essential roles beyond mechanical transport. It integrates into the mRNA export pathway collaborating with proteins like CRM1 (exportin-1) to regulate mRNA export. Additionally it plays a role in gene expression regulation by binding to gene promoters involving pathways linked to protein synthesis and cell cycle regulation. Connections with histone modifications suggest its involvement in chromatin remodeling linking it further to the regulation of gene expression pathways.

NUP98 associations are significant. It is implicated in certain hematological malignancies like acute myeloid leukemia (AML) where it forms fusion proteins with partners such as HOXA9 and NSD1. Genetic rearrangements involving NUP98 contribute to oncogenesis altering normal cell function. Other conditions including dysregulation in autoimmune diseases suggest potential links to aberrant signaling pathways. Understanding these disease links involves studying interactions with proteins like MLL another significant player in leukemia pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. NUP98 and NUP96 are involved in the bidirectional transport across the NPC (PubMed : 33097660). May anchor NUP153 and TPR to the NPC. In cooperation with DHX9, plays a role in transcription and alternative splicing activation of a subset of genes (PubMed : 28221134). Involved in the localization of DHX9 in discrete intranuclear foci (GLFG-body) (PubMed : 28221134).. (Microbial infection) Interacts with HIV-1 capsid protein P24 and nucleocapsid protein P7 and may thereby promote the integration of the virus in the host nucleus (in vitro) (PubMed : 23523133). Binding affinity to HIV-1 CA-NC complexes bearing the capsid change Asn-74-Asp is reduced (in vitro) (PubMed : 23523133).
See full target information NUP98
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Oncotarget 8:105440-105457 PubMed29285262

2017

Nuclear membrane-localised NOX4D generates pro-survival ROS in FLT3-ITD-expressing AML.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer N Moloney,Ashok Kumar Jayavelu,Joanna Stanicka,Sarah L Roche,Rebecca L O'Brien,Sebastian Scholl,Frank-D Böhmer,Thomas G Cotter

Monoclonal antibodies in immunodiagnosis and immunotherapy 32:81-90 PubMed23607342

2013

Monoclonal antibodies recognize gly-leu-phe-gly repeat of nucleoporin nup98 of tetrahymena, yeasts, and humans.

Applications

ICC/IF, WB, WB, WB, ICC/IF, ICC/IF, WB, ICC/IF

Species

Schizosaccharomyces pombe, Human, Tetrahymena, Saccharomyces cerevisiae S288C, Human, Tetrahymena, Schizosaccharomyces pombe, Saccharomyces cerevisiae S288C

Masaaki Iwamoto,Haruhiko Asakawa,Chizuru Ohtsuki,Hiroko Osakada,Takako Koujin,Yasushi Hiraoka,Tokuko Haraguchi
View all publications
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