AB179909

Anti-NUP98 antibody [21A10]

Name: Anti-NUP98 antibody [21A10] (ab179909) | Abcam
Brand: Abcam Limited
SKU: AB179909
Price: 905 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal NUP98 antibody. Suitable for WB, ICC/IF and reacts with Schizosaccharomyces pombe, Saccharomyces cerevisiae, Tetrahymena, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NUP98 aa 1-50.

View Alternative Names

ADAR2, NUP98, Nuclear pore complex protein Nup98-Nup96

8 Images

Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [21A10] (AB179909)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [21A10] (AB179909)

Immunofluorescence analysis of methanol-fixed HeLa cells, labeling NUP98 using ab179909 at 0.5 μg/ml, followed by Alexa Fluor 488-conjugated anti-mouse lgG (green) at 4 μg/ml. DAPI was used to stain DNA (magenta). Upper and middle panels correspond to black-and-white images while the bottom panel represents merged colored images.

Supplier Data

Western blot - Anti-NUP98 antibody [21A10] (AB179909)

Image shows unsuitability of this antibody for Human samples.

All lanes:

Western blot - Anti-NUP98 antibody [21A10] (ab179909) at 0.4 µg/mL

All lanes:

HeLa cell extract

Secondary

All lanes:

HRP-labeled anti-mouse IgG at 0.4 µg/mL

Predicted band size: 198 kDa

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ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [21A10] (AB179909)

Immunofluorescence analysis of formaldehyde-fixed, zymolyase-treated, S. pombe cells, labeling NUP98 using ab179909 at 10 μg/ml, followed by Alexa Fluor 488-conjugated anti-mouse lgG (green). DAPI was used to stain DNA (magenta). Upper and middle panels correspond to black-and-white images while the bottom panel represents merged images. Dotted lines represent the outlines of cells.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [21A10] (AB179909)

Immunofluorescence analysis of methanol-fixed Tetrahymena thermophila cells, labeling NUP98 using ab179909 at 0.5 μg/ml, followed by Alexa Fluor 488-conjugated anti-mouse lgG (green) at 4 μg/ml. DAPI was used to stain DNA (magenta). Upper and middle panels correspond to black-and-white images while the bottom panel represents merged images. Dotted lines represent the outlines of cells. The open arrow indicates the micronucleus. Insets are magnified images showing the position of the micronucleus.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [21A10] (AB179909)

Immunofluorescence analysis of formaldehyde-fixed, zymolyase-treated, S. cerevisiae cells, labeling NUP98 using ab179909 at 10 μg/ml, followed by Alexa Fluor 488-conjugated anti-mouse lgG (green). DAPI was used to stain DNA (magenta). Upper and middle panels correspond to black-and-white images while the bottom panel represents merged images. Dotted lines represent the outlines of cells.

Supplier Data

Western blot - Anti-NUP98 antibody [21A10] (AB179909)

All lanes:

Western blot - Anti-NUP98 antibody [21A10] (ab179909) at 2 µg/mL

Lane 1:

Cell extract from S. pombe wild type

Lane 2:

Cell extract from S. pombe expressing endogenously NUP98 fused to a fluorescence protein

Predicted band size: 198 kDa

true

Supplier Data

Western blot - Anti-NUP98 antibody [21A10] (AB179909)

All lanes:

Western blot - Anti-NUP98 antibody [21A10] (ab179909) at 2 µg/mL

All lanes:

S. cerevisiae cell extract

Predicted band size: 198 kDa

true

Supplier Data

Western blot - Anti-NUP98 antibody [21A10] (AB179909)

Open arrowheads correspond to NUP98. Asterisk represents uncharacterized protein. For lane 2 exposure time was about 10 times longer than for lane 1.

All lanes:

Western blot - Anti-NUP98 antibody [21A10] (ab179909) at 2 µg/mL

All lanes:

Tetrahymena thermophila cell extract

Predicted band size: 198 kDa

Observed band size: 98 kDa

true

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

21A10

Isotype

IgG1

Carrier free

Reacts with

Human, Saccharomyces cerevisiae, Tetrahymena, Schizosaccharomyces pombe

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Synthetic Peptide within Human NUP98 aa 600-700. Database link P52948

Epitope

GLF

Specificity

ab179909 crossreacts with multiple nucleoproteins of S. cerevisiae, e.g. Nup116, Nup100, Nup145N, Nup57 and Nup9.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "0.4-2 µg/mL", "WB-species-notes": "NOT suitable for Human samples.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.5-10 µg/mL", "ICCIF-species-notes": "" }, "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.4-2 µg/mL", "WB-species-notes": "NOT suitable for Human samples.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.5-10 µg/mL", "ICCIF-species-notes": "" }, "Schizosaccharomyces pombe": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.4-2 µg/mL", "WB-species-notes": "NOT suitable for Human samples.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.5-10 µg/mL", "ICCIF-species-notes": "" }, "Tetrahymena": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.4-2 µg/mL", "WB-species-notes": "NOT suitable for Human samples.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.5-10 µg/mL", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 6 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NUP98 protein also known as nucleoporin 98 plays an important role in cellular mechanics. It forms a part of the nuclear pore complex important for regulating nucleocytoplasmic transport. With an approximate mass of 98 kDa NUP98 expresses mainly in the nucleus across various cell types. Alternative splicing of the NUP98 gene can lead to different isoforms. In some research contexts NUP98 is also referred to by related terms such as NUP96. Its expression appears widespread in many tissues replacing or complementing other nucleoporins.

Biological function summary

The NUP98 protein contributes to nuclear-cytoplasmic transport processes as part of the nuclear pore complex. In association with other nucleoporins NUP98 acts in cargo transport between the nucleus and cytoplasm facilitating the movement of RNA and proteins. It also participates in the assembly and disassembly cycles of the nuclear pore complex (NPC). Known interactions include those with components like the 13C2 complex and other mRNA export factors. NUP98's function impacts NPC architecture and its transport efficiency.

Pathways

NUP98 has essential roles beyond mechanical transport. It integrates into the mRNA export pathway collaborating with proteins like CRM1 (exportin-1) to regulate mRNA export. Additionally it plays a role in gene expression regulation by binding to gene promoters involving pathways linked to protein synthesis and cell cycle regulation. Connections with histone modifications suggest its involvement in chromatin remodeling linking it further to the regulation of gene expression pathways.

NUP98 associations are significant. It is implicated in certain hematological malignancies like acute myeloid leukemia (AML) where it forms fusion proteins with partners such as HOXA9 and NSD1. Genetic rearrangements involving NUP98 contribute to oncogenesis altering normal cell function. Other conditions including dysregulation in autoimmune diseases suggest potential links to aberrant signaling pathways. Understanding these disease links involves studying interactions with proteins like MLL another significant player in leukemia pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. NUP98 and NUP96 are involved in the bidirectional transport across the NPC (PubMed : 33097660). May anchor NUP153 and TPR to the NPC. In cooperation with DHX9, plays a role in transcription and alternative splicing activation of a subset of genes (PubMed : 28221134). Involved in the localization of DHX9 in discrete intranuclear foci (GLFG-body) (PubMed : 28221134).. (Microbial infection) Interacts with HIV-1 capsid protein P24 and nucleocapsid protein P7 and may thereby promote the integration of the virus in the host nucleus (in vitro) (PubMed : 23523133). Binding affinity to HIV-1 CA-NC complexes bearing the capsid change Asn-74-Asp is reduced (in vitro) (PubMed : 23523133).

See full target information NUP98

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Monoclonal antibodies in immunodiagnosis and immunotherapy 32:81-90 PubMed23607342

2013

Monoclonal antibodies recognize gly-leu-phe-gly repeat of nucleoporin nup98 of tetrahymena, yeasts, and humans.

Applications

ICC/IF, WB, WB, ICC/IF, WB, WB, ICC/IF, ICC/IF

Species

Saccharomyces cerevisiae S288C, Saccharomyces cerevisiae S288C, Schizosaccharomyces pombe, Human, Tetrahymena, Human, Tetrahymena, Schizosaccharomyces pombe

Masaaki Iwamoto,Haruhiko Asakawa,Chizuru Ohtsuki,Hiroko Osakada,Takako Koujin,Yasushi Hiraoka,Tokuko Haraguchi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NUP98 antibody [21A10]

Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [21A10] (AB179909)

Western blot - Anti-NUP98 antibody [21A10] (AB179909)

Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [21A10] (AB179909)

Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [21A10] (AB179909)

Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [21A10] (AB179909)

Western blot - Anti-NUP98 antibody [21A10] (AB179909)

Western blot - Anti-NUP98 antibody [21A10] (AB179909)

Western blot - Anti-NUP98 antibody [21A10] (AB179909)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise