Anti-NUP98 antibody [2H10] - Nuclear Pore Marker

• ICC/IF

Collaborator

Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (AB50610)

ab50610 (1/100) staining NUP98 in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells (green).

Cells were fixed with paraformaldehyde/Triton X-100 [10 min in PTEMF buffer (20mM PIPES, 1mM MgCl₂, 10mM EGTA, 4% PFA) /0.2% Triton X-100 at room temperature] or methanol (6 min in methanol -20 °C , followed by 3 washes in 1x PBS) and counterstained with DAPI in order to highlight the nucleus (blue).

This image was generated using the ascites version of the product.

Image and protocol courtesy of Rosamaria Mangiacasale, Marilena Ciciarello and Patrizia Lavia, Univ Rome La Sapienza, Italy

• ICC/IF

AbReview42866****

Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (AB50610)

Paraformaldehyde-fixed, 0.5% Triton X-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells stained for NUP98 (green) using ab50610 at 1/200 dilution in ICC/IF, followed by Donkey Anti-Rat Alexa Fluor^® 488.

This image was generated using the ascites version of the product.

This image is courtesy of an anonymous Abreview.

• ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (AB50610)

Lentiviral vector-encoded shRNAs achieve efficient knock-down of human nucleoporins and have negligible cytotoxic or cytostatic effects.

HeLa cells (4×10⁶) were transduced with lentiviral vectors (MOI 50) encoding shRNAs specific for the indicated nucleoporins and used at 2 days p.t for Nup153 shRNA and 5 days p.t for all others.

(Panel B) Subcellular localisation of nuclear pore components upon nucleoporin knock-down was tested by confocal fluorescence microscopy of LV- (Control) and LV-shRNA transduced cells using specific anti-Nup antibodies. Images were acquired on the same day with the same conditions and are representative of two independent experiments.

This image was generated using the ascites version of the product.

Di Nunzio et al PLoS One. 2012;7(9):e46037. doi: 10.1371/journal.pone.0046037. Epub 2012 Sep 25. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (AB50610)

ab50610 (1/100) staining NUP98 in NIH/3T3 (Mouse embryo fibroblast cell line) cells (green).

Cells were fixed with paraformaldehyde/Triton X-100 (10 min in PTEMF buffer (20mM PIPES, 1mM MgCl₂, 10mM EGTA, 4% PFA) /0.2% Triton X-100 at room temperature) or methanol (6 min in methanol -20 °C , followed by 3 washes in 1x PBS) and counterstained with DAPI in order to highlight the nucleus (blue).

This image was generated using the ascites version of the product.

Image and protocol courtesy of Rosamaria Mangiacasale, Marilena Ciciarello and Patrizia Lavia, Univ Rome La Sapienza, Italy

• WB

Supplier Data

Western blot - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (AB50610)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610) at 1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear lysate

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate

Lane 4:

SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate

Lane 5:

COS-7 (african green monkey kidney fibroblast-like cell line) cell lysate

Lane 6:

NIH/3T3 (mouse embyro fibroblast cell line) cell lysate

Lane 7:

P19 cell lysate

Lane 8:

NRK cell lysate

Secondary

All lanes:

Goat Anti-Mouse IgG-Peroxidase

Predicted band size: 198 kDa

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