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Rat Monoclonal NUP98 antibody. Nuclear Pore marker. Suitable for WB, ICC/IF and reacts with Mouse, Rat, African green monkey, Human samples. Cited in 28 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NUP98 aa 1-500.


Images

Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (AB50610), expandable thumbnail
  • Western blot - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (AB50610), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (AB50610), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (AB50610), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (AB50610), expandable thumbnail

Publications

Key facts

Isotype
IgG2c
Host species
Rat
Storage buffer

pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human NUP98 aa 1-500. The exact immunogen used to generate this antibody is proprietary information. Database link P52948

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested
Mouse
Tested
Tested
Rat
Tested
Expected
African green monkey
Tested
Expected

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
African green monkey
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Customers have reported that Paraformaldehyde/Triton x-100 fixation provides better results, with sharp, regularly punctuate perinuclear signals. In MetOH fixed cells, the signal intensity can be somehwat lower and fuzzier and that single nucleoporin dots can be harder to distinguish around nuclear chromatin. Please see images below.

Species
Human
Dilution info
-
Notes

Customers have reported that Paraformaldehyde/Triton x-100 fixation provides better results, with sharp, regularly punctuate perinuclear signals. In MetOH fixed cells, the signal intensity can be somehwat lower and fuzzier and that single nucleoporin dots can be harder to distinguish around nuclear chromatin. Please see images below.

Expected
Expected

Species
Rat, African green monkey
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

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1 product for Alternative Version

Target data

Function

Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. NUP98 and NUP96 are involved in the bidirectional transport across the NPC (PubMed:33097660). May anchor NUP153 and TPR to the NPC. In cooperation with DHX9, plays a role in transcription and alternative splicing activation of a subset of genes (PubMed:28221134). Involved in the localization of DHX9 in discrete intranuclear foci (GLFG-body) (PubMed:28221134). (Microbial infection) Interacts with HIV-1 capsid protein P24 and nucleocapsid protein P7 and may thereby promote the integration of the virus in the host nucleus (in vitro) (PubMed:23523133). Binding affinity to HIV-1 CA-NC complexes bearing the capsid change Asn-74-Asp is reduced (in vitro) (PubMed:23523133).

Alternative names

ADAR2, NUP98, Nuclear pore complex protein Nup98-Nup96

Recommended products

Rat Monoclonal NUP98 antibody. Nuclear Pore marker. Suitable for WB, ICC/IF and reacts with Mouse, Rat, African green monkey, Human samples. Cited in 28 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NUP98 aa 1-500.

Key facts

Isotype
IgG2c
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human NUP98 aa 1-500. The exact immunogen used to generate this antibody is proprietary information. Database link P52948
Clone number
2H10
Purity
Tissue culture supernatant
Light chain type
kappa
Concentration
Loading...
Purification notes

Purified from TCS.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The NUP98 protein also known as nucleoporin 98 plays an important role in cellular mechanics. It forms a part of the nuclear pore complex important for regulating nucleocytoplasmic transport. With an approximate mass of 98 kDa NUP98 expresses mainly in the nucleus across various cell types. Alternative splicing of the NUP98 gene can lead to different isoforms. In some research contexts NUP98 is also referred to by related terms such as NUP96. Its expression appears widespread in many tissues replacing or complementing other nucleoporins.

Biological function summary

The NUP98 protein contributes to nuclear-cytoplasmic transport processes as part of the nuclear pore complex. In association with other nucleoporins NUP98 acts in cargo transport between the nucleus and cytoplasm facilitating the movement of RNA and proteins. It also participates in the assembly and disassembly cycles of the nuclear pore complex (NPC). Known interactions include those with components like the 13C2 complex and other mRNA export factors. NUP98's function impacts NPC architecture and its transport efficiency.

Pathways

NUP98 has essential roles beyond mechanical transport. It integrates into the mRNA export pathway collaborating with proteins like CRM1 (exportin-1) to regulate mRNA export. Additionally it plays a role in gene expression regulation by binding to gene promoters involving pathways linked to protein synthesis and cell cycle regulation. Connections with histone modifications suggest its involvement in chromatin remodeling linking it further to the regulation of gene expression pathways.

Associated diseases and disorders

NUP98 associations are significant. It is implicated in certain hematological malignancies like acute myeloid leukemia (AML) where it forms fusion proteins with partners such as HOXA9 and NSD1. Genetic rearrangements involving NUP98 contribute to oncogenesis altering normal cell function. Other conditions including dysregulation in autoimmune diseases suggest potential links to aberrant signaling pathways. Understanding these disease links involves studying interactions with proteins like MLL another significant player in leukemia pathogenesis.

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5 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610), expandable thumbnail
    Di Nunzio et al PLoS One. 2012;7(9):e46037. doi: 10.1371/journal.pone.0046037. Epub 2012 Sep 25. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610)

    Lentiviral vector-encoded shRNAs achieve efficient knock-down of human nucleoporins and have negligible cytotoxic or cytostatic effects.

    HeLa cells (4×106) were transduced with lentiviral vectors (MOI 50) encoding shRNAs specific for the indicated nucleoporins and used at 2 days p.t for Nup153 shRNA and 5 days p.t for all others.

    (Panel B) Subcellular localisation of nuclear pore components upon nucleoporin knock-down was tested by confocal fluorescence microscopy of LV- (Control) and LV-shRNA transduced cells using specific anti-Nup antibodies. Images were acquired on the same day with the same conditions and are representative of two independent experiments.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610), expandable thumbnail

    Western blot - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610)

    This image was generated using the ascites version of the product.

    All lanes: Western blot - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610) at 1 µg/mL

    Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear lysate

    Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

    Lane 3: Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate

    Lane 4: SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate

    Lane 5: COS-7 (african green monkey kidney fibroblast-like cell line) cell lysate

    Lane 6: NIH/3T3 (mouse embyro fibroblast cell line) cell lysate

    Lane 7: P19 cell lysate

    Lane 8: NRK cell lysate

    Secondary

    All lanes: Goat Anti-Mouse IgG-Peroxidase

    Developed using the ECL technique.

    Predicted band size: 198 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610), expandable thumbnail
    Image and protocol courtesy of Rosamaria Mangiacasale, Marilena Ciciarello and Patrizia Lavia, Univ Rome La Sapienza, Italy

    Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610)

    ab50610 (1/100) staining NUP98 in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells (green).

    Cells were fixed with paraformaldehyde/Triton X-100 [10 min in PTEMF buffer (20mM PIPES, 1mM MgCl2, 10mM EGTA, 4% PFA) /0.2% Triton X-100 at room temperature] or methanol (6 min in methanol -20 °C , followed by 3 washes in 1x PBS) and counterstained with DAPI in order to highlight the nucleus (blue).

    This image was generated using the ascites version of the product.

  • Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610), expandable thumbnail
    Image and protocol courtesy of Rosamaria Mangiacasale, Marilena Ciciarello and Patrizia Lavia, Univ Rome La Sapienza, Italy

    Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610)

    ab50610 (1/100) staining NUP98 in NIH/3T3 (Mouse embryo fibroblast cell line) cells (green).

    Cells were fixed with paraformaldehyde/Triton X-100 (10 min in PTEMF buffer (20mM PIPES, 1mM MgCl2, 10mM EGTA, 4% PFA) /0.2% Triton X-100 at room temperature) or methanol (6 min in methanol -20 °C , followed by 3 washes in 1x PBS) and counterstained with DAPI in order to highlight the nucleus (blue).

    This image was generated using the ascites version of the product.

  • Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610), expandable thumbnail
    This image is courtesy of an anonymous customer review.

    Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610)

    Paraformaldehyde-fixed, 0.5% Triton X-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells stained for NUP98 (green) using ab50610 at 1/200 dilution in ICC/IF, followed by Donkey Anti-Rat Alexa Fluor® 488.

    This image was generated using the ascites version of the product.

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