AB153914

Anti-NUR77 antibody

Name: Anti-NUR77 antibody (ab153914) | Abcam
Brand: Abcam Limited
SKU: AB153914
Price: 500 USD
Availability: InStock

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(14 Publications)

Anti-NUR77 antibody (ab153914) is a rabbit polyclonal antibody detecting NUR77 in Western Blot. Suitable for Human, Mouse.

View Alternative Names

GFRP1, HMR, NAK1, NR4A1, Nuclear receptor subfamily 4immunitygroup A member 1, Early response protein NAK1, Nuclear hormone receptor NUR/77, Orphan nuclear receptor HMR, Orphan nuclear receptor TR3, ST-59, Testicular receptor 3, Nur77

4 Images

Unknown

Western blot - Anti-NUR77 antibody (AB153914)

12% SDS PAGE

All lanes:

Western blot - Anti-NUR77 antibody (ab153914) at 1/500 dilution

Lane 1:

Non-transfected 293T cell lysate

Lane 2:

NR4A1-transfected 293T cell lysate

Predicted band size: 64 kDa

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Supplier Data

Western blot - Anti-NUR77 antibody (AB153914)

Samples were separated by 7.5% SDS PAGE.

All lanes:

Western blot - Anti-NUR77 antibody (ab153914) at 1/500 dilution

Lane 1:

Untreated Jurkat whole cell extracts at 30 µg

Lane 2:

Jurkat whole cell extracts treated with 300ng/ml BFA for 45 mins and 40nMTPA and 2µM A23187, 16hr at 30 µg

Lane 3:

Jurkat whole cell extracts treated with 40nMTPA and 2µM A23187, 2hr at 30 µg

Lane 4:

Jurkat whole cell extracts treated with 40nMTPA and 2µM A23187, 4hr at 30 µg

Lane 5:

Jurkat whole cell extracts treated with 40nMTPA and 2µM A23187, 6hr at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 64 kDa

false

Supplier Data

Western blot - Anti-NUR77 antibody (AB153914)

Samples were separated by 7.5% SDS PAGE.

All lanes:

Western blot - Anti-NUR77 antibody (ab153914) at 1/500 dilution

Lane 1:

Untreated HepG2 cell lysates at 30 µg

Lane 2:

HepG2 treated with 1% Hypoxia, 24hr at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 64 kDa

false

Unknown

Western blot - Anti-NUR77 antibody (AB153914)

7.5% SDS PAGE

All lanes:

Western blot - Anti-NUR77 antibody (ab153914) at 1/1000 dilution

All lanes:

mouse liver whole cell lysate at 50 µg

Predicted band size: 64 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human NR4A1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P22736

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-NUR77 antibody (ab153914) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse samples.

What is the molecular weight of NUR77?
Anti-NUR77 (ab153914) specifically detects a band for NUR77 (UniProt: P22736) at a molecular weight of 64kDa.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NUR77 also known as NR4A1 is an orphan nuclear receptor and transcription factor with a molecular mass of around 65 kDa. This protein plays an important role in mediating gene expression by binding DNA at specific response elements. It is expressed in various tissues including the thymus spleen and central nervous system. NUR77 achieves its mechanical function by regulating gene transcription involved in cell proliferation apoptosis and metabolism.

Biological function summary

The functions of NUR77 impact multiple cellular processes that are essential for maintaining cellular homeostasis. As part of its biological roles NUR77 can form complexes with other nuclear receptors and influence gene expression. It is particularly significant in T-cell receptor signaling where it influences the balance between cell survival and cell death. This modulation helps in the regulation of immune functions and cellular development.

Pathways

NUR77 integrates into the MAPK signaling and apoptosis pathways. It interacts with proteins such as Bcl-2 and p53 facilitating cellular responses to stress and developmental cues. In the MAPK pathway NUR77 can modulate transcriptional activities affecting cell proliferation and differentiation while in apoptotic pathways it contributes to the regulation of programmed cell death making it a critical modulator in these contexts.

NUR77 has been associated with cancer and atherosclerosis. Its role in apoptosis makes it a point of interest in cancer research where altered NUR77 expression can influence tumor growth and resistance to therapy. In the context of atherosclerosis aberrant activity of NUR77 affects lipid metabolism and inflammation. Through its link to pathways involving Bcl-2 and p53 NUR77 remains a significant target in both therapeutic research and disease management.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Orphan nuclear receptor. Binds the NGFI-B response element (NBRE) 5'-AAAGGTCA-3' (PubMed : 18690216, PubMed : 8121493, PubMed : 9315652). Binds 9-cis-retinoic acid outside of its ligand-binding (NR LBD) domain (PubMed : 18690216). Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation (PubMed : 22983157). Regulates the inflammatory response in macrophages by regulating metabolic adaptations during inflammation, including repressing the transcription of genes involved in the citric acid cycle (TCA) (By similarity). Inhibits NF-kappa-B signaling by binding to low-affinity NF-kappa-B binding sites, such as at the IL2 promoter (PubMed : 15466594). May act concomitantly with NR4A2 in regulating the expression of delayed-early genes during liver regeneration (By similarity). Plays a role in the vascular response to injury (By similarity).. In the cytosol, upon its detection of both bacterial lipopolysaccharide (LPS) and NBRE-containing mitochondrial DNA released by GSDMD pores during pyroptosis, it promotes non-canonical NLRP3 inflammasome activation by stimulating association of NLRP3 and NEK7.

See full target information NR4A1

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Gut microbes 17:2518338 PubMed40596758

2025

Gut microbial Nordihydroguaiaretic acid suppresses macrophage pyroptosis to regulate epithelial homeostasis and inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Wang,Huishi Tan,Ziwen Ye,Senhui Weng,Yanqiang Shi,Jiahui Xu,Hongbin Liu,Jierui Li,Linwen Huang,Luyue Zhai,Huishan Luo,Zelong Lin,Cailing Zhong,Jing Tang,Zezheng Wang,Haiyan Zhang,Beiping Zhang,Chongyang Huang

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 39:e70771 PubMed40569148

2025

Vasopressin-Sensitive Aqp2 Regulation Mediated by the TAZ-NR4A1 Axis in Renal Collecting Duct Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Seok Choi,Hyo-Ju Jang,Wan-Young Kim,Sun Ah Park,Euijung Park,Hyun Jun Jung,Tae-Hwan Kwon

Translational neurodegeneration 14:29 PubMed40468463

2025

Aging-dependent YAP1 reduction contributes to AD pathology by upregulating the Nr4a1-AKT/GSK-3β axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Lei,Yilei Cheng,Anqi Yin,Jian-Min Han,Gang Wu,Fumin Yang,Qi Wang,Jian-Zhi Wang,Rong Liu,Hong-Lian Li,Xiaochuan Wang

Brain and behavior 15:e70377 PubMed40320842

2025

Analysis and Identification of Therapeutic Targets for Neuronal Regeneration After Ischemic Stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Li Min,Li Guo,Zhenyu Wang,Lei Zhao,Chenglong Shi,Xiaoyong Liu,Zhen Wang,Fei-Fei Shang,Jiaping Wang

eLife 13: PubMed39998486

2025

A VgrG2b fragment cleaved by caspase-11/4 promotes infection through suppressing the NLRP3 inflammasome.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Qian,Qiannv Liu,Xiangyun Cheng,Chunlei Wang,Chun Kong,Mengqian Li,Chao Ren,Dong Jiang,Shuo Wang,Pengyan Xia

European journal of medical research 30:117 PubMed39972514

2025

MEK5-ERK5 pathway mediates mitophagy by regulating Nur77 to promote tumorigenesis of osteosarcoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jianshu Wang,Jinxu Xue,Baijing Ma,Yanqi Zhu,Jing Li,Caiping Tian

The Journal of experimental medicine 221: PubMed38334978

2024

PROTAC-mediated NR4A1 degradation as a novel strategy for cancer immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Wang,Yufeng Xiao,Yuewan Luo,Rohan P Master,Jiao Mo,Myung-Chul Kim,Yi Liu,Chandra K Maharjan,Urvi M Patel,Umasankar De,Madison E Carelock,Tanzia Islam Tithi,Xiangming Li,Donald R Shaffer,Kevin R Guertin,Haoyang Zhuang,Emily Moser,Keiran S M Smalley,Dongwen Lv,Daohong Zhou,Guangrong Zheng,Weizhou Zhang

Cell 186:3706-3725.e29 PubMed37562402

2023

Distinct molecular profiles of skull bone marrow in health and neurological disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Zeynep Ilgin Kolabas,Louis B Kuemmerle,Robert Perneczky,Benjamin Förstera,Selin Ulukaya,Mayar Ali,Saketh Kapoor,Laura M Bartos,Maren Büttner,Ozum Sehnaz Caliskan,Zhouyi Rong,Hongcheng Mai,Luciano Höher,Denise Jeridi,Muge Molbay,Igor Khalin,Ioannis K Deligiannis,Moritz Negwer,Kenny Roberts,Alba Simats,Olga Carofiglio,Mihail I Todorov,Izabela Horvath,Furkan Ozturk,Selina Hummel,Gloria Biechele,Artem Zatcepin,Marcus Unterrainer,Johannes Gnörich,Jay Roodselaar,Joshua Shrouder,Pardis Khosravani,Benjamin Tast,Lisa Richter,Laura Díaz-Marugán,Doris Kaltenecker,Laurin Lux,Ying Chen,Shan Zhao,Boris-Stephan Rauchmann,Michael Sterr,Ines Kunze,Karen Stanic,Vanessa W Y Kan,Simon Besson-Girard,Sabrina Katzdobler,Carla Palleis,Julia Schädler,Johannes C Paetzold,Sabine Liebscher,Anja E Hauser,Ozgun Gokce,Heiko Lickert,Hanno Steinke,Corinne Benakis,Christian Braun,Celia P Martinez-Jimenez,Katharina Buerger,Nathalie L Albert,Günter Höglinger,Johannes Levin,Christian Haass,Anna Kopczak,Martin Dichgans,Joachim Havla,Tania Kümpfel,Martin Kerschensteiner,Martina Schifferer,Mikael Simons,Arthur Liesz,Natalie Krahmer,Omer A Bayraktar,Nicolai Franzmeier,Nikolaus Plesnila,Suheda Erener,Victor G Puelles,Claire Delbridge,Harsharan Singh Bhatia,Farida Hellal,Markus Elsner,Ingo Bechmann,Benjamin Ondruschka,Matthias Brendel,Fabian J Theis,Ali Erturk

Biology 12: PubMed37626950

2023

Structural Adaptation of the Excitation-Contraction Coupling Apparatus in Calsequestrin1-Null Mice during Postnatal Development.

Applications

Unspecified application

Species

Unspecified reactive species

Stefania Murzilli,Matteo Serano,Laura Pietrangelo,Feliciano Protasi,Cecilia Paolini

British journal of pharmacology 180:2577-2598 PubMed37263753

2023

Emodin promotes hepatic stellate cell senescence and alleviates liver fibrosis via a nuclear receptor (Nur77)-mediated epigenetic regulation of glutaminase 1.

Applications

Unspecified application

Species

Unspecified reactive species

Li Chen,Baoyu Liang,Siwei Xia,Feixia Wang,Zhanghao Li,Jiangjuan Shao,Zili Zhang,Anping Chen,Shizhong Zheng,Feng Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-NUR77 antibody

Western blot - Anti-NUR77 antibody (AB153914)

Western blot - Anti-NUR77 antibody (AB153914)

Western blot - Anti-NUR77 antibody (AB153914)

Western blot - Anti-NUR77 antibody (AB153914)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

Product promise