Anti-NUR77 antibody (ab153914) is a rabbit polyclonal antibody that is used to detect NUR77 in Western Blot. Suitable for Human, Mouse samples.
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- British journal of pharmacology 180:2577-25982023Emodin promotes hepatic stellate cell senescence and alleviates liver fibrosis via a nuclear receptor (Nur77)-mediated epigenetic regulation of glutaminase 1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Chen,Baoyu Liang,Siwei Xia,Feixia Wang,Zhanghao Li,Jiangjuan Shao,Zili Zhang,Anping Chen,Shizhong Zheng,Feng ZhangPubMed 37263753
- Cell death discovery 9:1062023Caspase 6/NR4A1/SOX9 signaling axis regulates hepatic inflammation and pyroptosis in ischemia-stressed fatty liver.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingwei Sheng,Yiqi Weng,Yingli Cao,Chen Zhang,Yuanbang Lin,Wenli YuPubMed 36977670
- Oxidative medicine and cellular longevity 2022:20178152022Nur77 Deficiency Exacerbates Macrophage NLRP3 Inflammasome-Mediated Inflammation and Accelerates Atherosclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuosen Yuan,Weifeng Zhang,Peng Nie,Keke Lan,Xiaoxiao Yang,Anwen Yin,Qingqing Xiao,Yejiao Shen,Ke Xu,Xia Wang,Xin Pan,Linghong Shen,Ben HePubMed 35464766
- Molecular psychiatry 28:497-5142022Differential vulnerability of adult neurogenic niches to dosage of the neurodevelopmental-disorder linked gene Foxg1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIris Schäffner,Marie-Theres Wittmann,Tanja Vogel,D Chichung LiePubMed 35318461
- Experimental and molecular pathology 111:1043032019NR4A1 silencing protects against renal ischemia-reperfusion injury through activation of the β-catenin signaling pathway in old mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjian Shi et. al.PubMed 31465766
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human NR4A1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P22736
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Not recommended |
| Mouse | Tested | Not recommended |
| Rat | Predicted | Not recommended |
| Cow | Predicted | Not recommended |
| Dog | Predicted | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Dog | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Cow, Dog | Dilution info - | Notes - |
Associated Products
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Target data
Function
Orphan nuclear receptor. Binds the NGFI-B response element (NBRE) 5'-AAAGGTCA-3' (PubMed:18690216, PubMed:8121493, PubMed:9315652). Binds 9-cis-retinoic acid outside of its ligand-binding (NR LBD) domain (PubMed:18690216). Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation (PubMed:22983157). Regulates the inflammatory response in macrophages by regulating metabolic adaptations during inflammation, including repressing the transcription of genes involved in the citric acid cycle (TCA) (By similarity). Inhibits NF-kappa-B signaling by binding to low-affinity NF-kappa-B binding sites, such as at the IL2 promoter (PubMed:15466594). May act concomitantly with NR4A2 in regulating the expression of delayed-early genes during liver regeneration (By similarity). Plays a role in the vascular response to injury (By similarity). In the cytosol, upon its detection of both bacterial lipopolysaccharide (LPS) and NBRE-containing mitochondrial DNA released by GSDMD pores during pyroptosis, it promotes non-canonical NLRP3 inflammasome activation by stimulating association of NLRP3 and NEK7.
Alternative names
GFRP1, HMR, NAK1, NR4A1, Nuclear receptor subfamily 4immunitygroup A member 1, Early response protein NAK1, Nuclear hormone receptor NUR/77, Orphan nuclear receptor HMR, Orphan nuclear receptor TR3, ST-59, Testicular receptor 3, Nur77
Recommended products
Anti-NUR77 antibody (ab153914) is a rabbit polyclonal antibody that is used to detect NUR77 in Western Blot. Suitable for Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human NR4A1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P22736
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-NUR77 antibody (ab153914) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse samples.
What is the molecular weight of NUR77?
Anti-NUR77 (ab153914) specifically detects a band for NUR77 (UniProt: P22736) at a molecular weight of 64kDa.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NUR77 also known as NR4A1 is an orphan nuclear receptor and transcription factor with a molecular mass of around 65 kDa. This protein plays an important role in mediating gene expression by binding DNA at specific response elements. It is expressed in various tissues including the thymus spleen and central nervous system. NUR77 achieves its mechanical function by regulating gene transcription involved in cell proliferation apoptosis and metabolism.
- Biological function summary
The functions of NUR77 impact multiple cellular processes that are essential for maintaining cellular homeostasis. As part of its biological roles NUR77 can form complexes with other nuclear receptors and influence gene expression. It is particularly significant in T-cell receptor signaling where it influences the balance between cell survival and cell death. This modulation helps in the regulation of immune functions and cellular development.
- Pathways
NUR77 integrates into the MAPK signaling and apoptosis pathways. It interacts with proteins such as Bcl-2 and p53 facilitating cellular responses to stress and developmental cues. In the MAPK pathway NUR77 can modulate transcriptional activities affecting cell proliferation and differentiation while in apoptotic pathways it contributes to the regulation of programmed cell death making it a critical modulator in these contexts.
- Associated diseases and disorders
NUR77 has been associated with cancer and atherosclerosis. Its role in apoptosis makes it a point of interest in cancer research where altered NUR77 expression can influence tumor growth and resistance to therapy. In the context of atherosclerosis aberrant activity of NUR77 affects lipid metabolism and inflammation. Through its link to pathways involving Bcl-2 and p53 NUR77 remains a significant target in both therapeutic research and disease management.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-NUR77 antibody (ab153914)
12% SDS PAGE
All lanes: Western blot - Anti-NUR77 antibody (ab153914) at 1/500 dilution
Lane 1: Non-transfected 293T cell lysate
Lane 2: NR4A1-transfected 293T cell lysate
Predicted band size: 64 kDa
Western blot - Anti-NUR77 antibody (ab153914)
7.5% SDS PAGE
All lanes: Western blot - Anti-NUR77 antibody (ab153914) at 1/1000 dilution
All lanes: mouse liver whole cell lysate at 50 µg
Predicted band size: 64 kDa
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