Rabbit Polyclonal NUR77 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NR4A1 aa 350 to C-terminus.
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Publications
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- British journal of pharmacology 180:2577-25982023Emodin promotes hepatic stellate cell senescence and alleviates liver fibrosis via a nuclear receptor (Nur77)-mediated epigenetic regulation of glutaminase 1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Chen,Baoyu Liang,Siwei Xia,Feixia Wang,Zhanghao Li,Jiangjuan Shao,Zili Zhang,Anping Chen,Shizhong Zheng,Feng ZhangPubMed 37263753
- Cell death discovery 9:1062023Caspase 6/NR4A1/SOX9 signaling axis regulates hepatic inflammation and pyroptosis in ischemia-stressed fatty liver.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingwei Sheng,Yiqi Weng,Yingli Cao,Chen Zhang,Yuanbang Lin,Wenli YuPubMed 36977670
- Oxidative medicine and cellular longevity 2022:20178152022Nur77 Deficiency Exacerbates Macrophage NLRP3 Inflammasome-Mediated Inflammation and Accelerates Atherosclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuosen Yuan,Weifeng Zhang,Peng Nie,Keke Lan,Xiaoxiao Yang,Anwen Yin,Qingqing Xiao,Yejiao Shen,Ke Xu,Xia Wang,Xin Pan,Linghong Shen,Ben HePubMed 35464766
- Molecular psychiatry 28:497-5142022Differential vulnerability of adult neurogenic niches to dosage of the neurodevelopmental-disorder linked gene Foxg1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIris Schäffner,Marie-Theres Wittmann,Tanja Vogel,D Chichung LiePubMed 35318461
- Experimental and molecular pathology 111:1043032019NR4A1 silencing protects against renal ischemia-reperfusion injury through activation of the β-catenin signaling pathway in old mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjian Shi et. al.PubMed 31465766
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human NR4A1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P22736
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Not recommended |
| Mouse | Tested | Not recommended |
| Rat | Predicted | Not recommended |
| Cow | Predicted | Not recommended |
| Dog | Predicted | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Dog | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Cow, Dog | Dilution info - | Notes - |
Associated Products
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3 products for Alternative Product
Target data
Function
Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3' (By similarity). May inhibit NF-kappa-B transactivation of IL2. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation. Plays a role in the vascular response to injury (By similarity).
Alternative names
Nuclear receptor subfamily 4 group A member 1, Early response protein NAK1, Nuclear hormone receptor NUR/77, Orphan nuclear receptor HMR, Orphan nuclear receptor TR3, ST-59, Testicular receptor 3, Nur77, HMR, GFRP1, NAK1, NR4A1
Recommended products
Rabbit Polyclonal NUR77 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NR4A1 aa 350 to C-terminus.
Alternative names
Nuclear receptor subfamily 4 group A member 1, Early response protein NAK1, Nuclear hormone receptor NUR/77, Orphan nuclear receptor HMR, Orphan nuclear receptor TR3, ST-59, Testicular receptor 3, Nur77, HMR, GFRP1, NAK1, NR4A1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human NR4A1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P22736
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NUR77 also known as NR4A1 is an orphan nuclear receptor and transcription factor with a molecular mass of around 65 kDa. This protein plays an important role in mediating gene expression by binding DNA at specific response elements. It is expressed in various tissues including the thymus spleen and central nervous system. NUR77 achieves its mechanical function by regulating gene transcription involved in cell proliferation apoptosis and metabolism.
- Biological function summary
The functions of NUR77 impact multiple cellular processes that are essential for maintaining cellular homeostasis. As part of its biological roles NUR77 can form complexes with other nuclear receptors and influence gene expression. It is particularly significant in T-cell receptor signaling where it influences the balance between cell survival and cell death. This modulation helps in the regulation of immune functions and cellular development.
- Pathways
NUR77 integrates into the MAPK signaling and apoptosis pathways. It interacts with proteins such as Bcl-2 and p53 facilitating cellular responses to stress and developmental cues. In the MAPK pathway NUR77 can modulate transcriptional activities affecting cell proliferation and differentiation while in apoptotic pathways it contributes to the regulation of programmed cell death making it a critical modulator in these contexts.
- Associated diseases and disorders
NUR77 has been associated with cancer and atherosclerosis. Its role in apoptosis makes it a point of interest in cancer research where altered NUR77 expression can influence tumor growth and resistance to therapy. In the context of atherosclerosis aberrant activity of NUR77 affects lipid metabolism and inflammation. Through its link to pathways involving Bcl-2 and p53 NUR77 remains a significant target in both therapeutic research and disease management.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-NUR77 antibody (ab153914)
12% SDS PAGE
All lanes: Western blot - Anti-NUR77 antibody (ab153914) at 1/500 dilution
Lane 1: Non-transfected 293T cell lysate
Lane 2: NR4A1-transfected 293T cell lysate
Predicted band size: 64 kDa
Western blot - Anti-NUR77 antibody (ab153914)
7.5% SDS PAGE
All lanes: Western blot - Anti-NUR77 antibody (ab153914) at 1/1000 dilution
All lanes: mouse liver whole cell lysate at 50 µg
Predicted band size: 64 kDa
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