JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB317395

Anti-NUR77 antibody [S02-1C7]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Monoclonal NUR77 antibody. Suitable for WB and reacts with Rat samples. Immunogen corresponding to Synthetic Peptide within Human NR4A1 aa 1-100.

View Alternative Names

Hmr, Ngfib, Nuclear receptor subfamily 4 group A member 1, NUR77, Nerve growth factor-induced protein I-B, Orphan nuclear receptor HMR, NGFI-B

1 Images
Western blot - Anti-NUR77 Antibody [S02-1C7] (AB317395)
  • WB

Supplier Data

Western blot - Anti-NUR77 Antibody [S02-1C7] (AB317395)

All lanes:

Western blot - Anti-NUR77 Antibody [S02-1C7] (ab317395) at 1/500 dilution

All lanes:

rat brain lysate

Predicted band size: 64 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

S02-1C7

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

WB

applications

Immunogen

Synthetic Peptide within Human NR4A1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P22736

style
left-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
Primary Antibodies

AB41917

Anti-Nurr1 antibody [N1404]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nurr1 antibody [N1404] (AB41917)

  • 4
  • 9
Proteins & Peptides

AB114315

Recombinant Human NUR77 protein

SDS-PAGE - Recombinant Human NUR77 protein (AB114315)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.01% Sodium azide Constituents: 55.08% Water, 40% Glycerol (glycerin, glycerine), 0.98% Tris glycine, 0.88% Sodium chloride, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NUR77 also known as NR4A1 is an orphan nuclear receptor and transcription factor with a molecular mass of around 65 kDa. This protein plays an important role in mediating gene expression by binding DNA at specific response elements. It is expressed in various tissues including the thymus spleen and central nervous system. NUR77 achieves its mechanical function by regulating gene transcription involved in cell proliferation apoptosis and metabolism.
Biological function summary

The functions of NUR77 impact multiple cellular processes that are essential for maintaining cellular homeostasis. As part of its biological roles NUR77 can form complexes with other nuclear receptors and influence gene expression. It is particularly significant in T-cell receptor signaling where it influences the balance between cell survival and cell death. This modulation helps in the regulation of immune functions and cellular development.

Pathways

NUR77 integrates into the MAPK signaling and apoptosis pathways. It interacts with proteins such as Bcl-2 and p53 facilitating cellular responses to stress and developmental cues. In the MAPK pathway NUR77 can modulate transcriptional activities affecting cell proliferation and differentiation while in apoptotic pathways it contributes to the regulation of programmed cell death making it a critical modulator in these contexts.

NUR77 has been associated with cancer and atherosclerosis. Its role in apoptosis makes it a point of interest in cancer research where altered NUR77 expression can influence tumor growth and resistance to therapy. In the context of atherosclerosis aberrant activity of NUR77 affects lipid metabolism and inflammation. Through its link to pathways involving Bcl-2 and p53 NUR77 remains a significant target in both therapeutic research and disease management.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Orphan nuclear receptor. Binds the NGFI-B response element (NBRE) 5'-AAAGGTCA-3' (PubMed : 10331876). Binds 9-cis-retinoic acid outside of its ligand-binding (NR LBD) domain (By similarity). Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation (By similarity). Regulates the inflammatory response in macrophages by regulating metabolic adaptations during inflammation, including repressing the transcription of genes involved in the citric acid cycle (TCA) (By similarity). Inhibits NF-kappa-B signaling by binding to low-affinity NF-kappa-B binding sites, such as at the IL2 promoter (By similarity). May act concomitantly with NR4A2 in regulating the expression of delayed-early genes during liver regeneration (PubMed : 8473329). Plays a role in the vascular response to injury (By similarity).. In the cytosol, upon its detection of both bacterial lipopolysaccharide (LPS) and NBRE-containing mitochondrial DNA released by GSDMD pores during pyroptosis, it promotes non-canonical NLRP3 inflammasome activation by stimulating association of NLRP3 and NEK7.
See full target information Nr4a1
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com