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AB111962

Anti-NUR77 (phospho S351) antibody

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(3 Publications)

Rabbit Polyclonal NUR77 phospho S351 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human NR4A1 phospho S351.

View Alternative Names

GFRP1, HMR, NAK1, NR4A1, Nuclear receptor subfamily 4immunitygroup A member 1, Early response protein NAK1, Nuclear hormone receptor NUR/77, Orphan nuclear receptor HMR, Orphan nuclear receptor TR3, ST-59, Testicular receptor 3, Nur77

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUR77 (phospho S351) antibody (AB111962)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUR77 (phospho S351) antibody (AB111962)

ab111962 at 1/50 dilution staining NUR77 in paraffin-embedded Human Colon carcinoma tissue by Immunohistochemistry. The image on the right is treated with the synthesized phosphopeptide.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human NR4A1 phospho S351. The exact immunogen used to generate this antibody is proprietary information.

P22736

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab111962 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NUR77 also known as NR4A1 is an orphan nuclear receptor and transcription factor with a molecular mass of around 65 kDa. This protein plays an important role in mediating gene expression by binding DNA at specific response elements. It is expressed in various tissues including the thymus spleen and central nervous system. NUR77 achieves its mechanical function by regulating gene transcription involved in cell proliferation apoptosis and metabolism.
Biological function summary

The functions of NUR77 impact multiple cellular processes that are essential for maintaining cellular homeostasis. As part of its biological roles NUR77 can form complexes with other nuclear receptors and influence gene expression. It is particularly significant in T-cell receptor signaling where it influences the balance between cell survival and cell death. This modulation helps in the regulation of immune functions and cellular development.

Pathways

NUR77 integrates into the MAPK signaling and apoptosis pathways. It interacts with proteins such as Bcl-2 and p53 facilitating cellular responses to stress and developmental cues. In the MAPK pathway NUR77 can modulate transcriptional activities affecting cell proliferation and differentiation while in apoptotic pathways it contributes to the regulation of programmed cell death making it a critical modulator in these contexts.

NUR77 has been associated with cancer and atherosclerosis. Its role in apoptosis makes it a point of interest in cancer research where altered NUR77 expression can influence tumor growth and resistance to therapy. In the context of atherosclerosis aberrant activity of NUR77 affects lipid metabolism and inflammation. Through its link to pathways involving Bcl-2 and p53 NUR77 remains a significant target in both therapeutic research and disease management.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Orphan nuclear receptor. Binds the NGFI-B response element (NBRE) 5'-AAAGGTCA-3' (PubMed : 18690216, PubMed : 8121493, PubMed : 9315652). Binds 9-cis-retinoic acid outside of its ligand-binding (NR LBD) domain (PubMed : 18690216). Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation (PubMed : 22983157). Regulates the inflammatory response in macrophages by regulating metabolic adaptations during inflammation, including repressing the transcription of genes involved in the citric acid cycle (TCA) (By similarity). Inhibits NF-kappa-B signaling by binding to low-affinity NF-kappa-B binding sites, such as at the IL2 promoter (PubMed : 15466594). May act concomitantly with NR4A2 in regulating the expression of delayed-early genes during liver regeneration (By similarity). Plays a role in the vascular response to injury (By similarity).. In the cytosol, upon its detection of both bacterial lipopolysaccharide (LPS) and NBRE-containing mitochondrial DNA released by GSDMD pores during pyroptosis, it promotes non-canonical NLRP3 inflammasome activation by stimulating association of NLRP3 and NEK7.
See full target information NR4A1 phospho S351
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:19 PubMed39809736

2025

PKCα regulates the secretion of PDL1-carrying small extracellular vesicles in a p53-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Ren Zhang,Weilin Liao,Xi Chen,Junyi Wang,Jiaqi Li,Geer Chen,Weiyu Wu,Xiaoxuan Wang,Yao Zhang,Ziyu Chen,Xiaoyu Zhu,Zicong Lin,Yizhun Zhu,Lijuan Ma,Haijie Yu

Molecular cell 81:4041-4058.e15 PubMed34624217

2021

NR4A1 regulates expression of immediate early genes, suppressing replication stress in cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hongshan Guo,Gabriel Golczer,Ben S Wittner,Adam Langenbucher,Marcus Zachariah,Taronish D Dubash,Xin Hong,Valentine Comaills,Risa Burr,Richard Y Ebright,Elad Horwitz,Joanna A Vuille,Soroush Hajizadeh,Devon F Wiley,Brittany A Reeves,Jia-Min Zhang,Kira L Niederhoffer,Chenyue Lu,Benjamin Wesley,Uyen Ho,Linda T Nieman,Mehmet Toner,Shobha Vasudevan,Lee Zou,Raul Mostoslavsky,Shyamala Maheswaran,Michael S Lawrence,Daniel A Haber

Cell reports 33:108284 PubMed33086070

2020

A Regulation Loop between YAP and NR4A1 Balances Cell Proliferation and Apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Lingli He,Liang Yuan,Wentao Yu,Yang Sun,Dan Jiang,Xiaodong Wang,Xue Feng,Zuoyun Wang,Jinjin Xu,Ruizeng Yang,Wenxiang Zhang,Hua Feng,Hang-Zi Chen,Yi Arial Zeng,Lijian Hui,Qiao Wu,Yonglong Zhang,Lei Zhang
View all publications
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Product promise

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