AB54366

Anti-Nurr1 antibody [447C2a]

Name: Anti-Nurr1 antibody [447C2a] (ab54366) | Abcam
Brand: Abcam Limited
SKU: AB54366

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(2 Publications)

Mouse Monoclonal Nurr1 antibody. Suitable for WB and reacts with Recombinant fragment samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NR4A2.

View Alternative Names

NOT, NURR1, TINUR, NR4A2, Nuclear receptor subfamily 4 group A member 2, Immediate-early response protein NOT, Orphan nuclear receptor NURR1, Transcriptionally-inducible nuclear receptor

1 Images

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Western blot - Anti-Nurr1 antibody [447C2a] (AB54366)

All lanes:

Western blot - Anti-Nurr1 antibody [447C2a] (ab54366)

All lanes:

immunised recombinant protein

Predicted band size: 67 kDa

Observed band size: 37 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

447C2a

Isotype

IgG1

Carrier free

Applications

applications

Immunogen

Recombinant Fragment Protein within Human NR4A2. The exact immunogen used to generate this antibody is proprietary information.

P43354

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

ab54366 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.03% Tripotassium orthophosphate, 0.0225% Potassium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nuclear receptor-related 1 protein (Nurr1) is a transcription factor weighing approximately 66 kDa. Scientists also know Nurr1 as NR4A2 and it belongs to the NR4A nuclear receptor family. Nurr1 is mainly expressed in the central nervous system where it plays a significant role in neuronal development and maintenance. Despite lacking a defined ligand-binding activity Nurr1 regulates gene expression directly by binding to DNA.

Biological function summary

Nurr1 functions as a critical regulator of dopaminergic neurons in the brain. It is vital for the survival and differentiation of these neurons which are important for motor control and behavioral responses. Nurr1 partners with other transcription factors often functioning in complexes that modulate brain-derived neurotrophic factor (BDNF) signaling. It influences the transcription of genes involved in inflammatory responses and neuroprotection reflecting its importance in maintaining neural health.

Pathways

Nurr1 integrates into key biological processes involving dopaminergic signaling and neuroinflammation. Nurr1 directly participates in the Wnt signaling pathway which plays a role in neuronal development and synaptic function. It also interacts with proteins such as Pitx3 and LMX1B within these pathways contributing to the regulation of dopaminergic neuron function and survival ultimately affecting broader central nervous system activity.

Nurr1 has strong associations with neurological conditions such as Parkinson’s disease and schizophrenia. Its impaired function or altered expression can lead to dopaminergic neuron degeneration a hallmark of Parkinson's disease. Research indicates Nurr1's interaction with parkin a protein implicated in the disease's pathology further emphasizes its importance. In schizophrenia altered Nurr1 activity affects synaptic regulation and cognitive function potentially relating to disrupted dopaminergic and BDNF pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development (PubMed : 15716272, PubMed : 17184956). It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons (By similarity).

See full target information NR4A2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of clinical and translational hepatology 11:787-799 PubMed37408817

2023

IGF2-NR4A2 Signaling Regulates Macrophage Subtypes to Attenuate Liver Cirrhosis.

Applications

Unspecified application

Species

Unspecified reactive species

Lichao Yao,Xue Hu,Mengqin Yuan,Qiuling Zhang,Pingji Liu,Lian Yang,Kai Dai,Yingan Jiang

Molecular medicine reports 12:5487-93 PubMed26239742

2015

Decreased expression levels of Nurr1 are associated with chronic inflammation in patients with type 2 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Xu,Qi Huang,Wenfang Zhang,Yaping Wang,Qingling Zeng,Chunyan He,Junli Xue,Jin Chen,Xuemei Hu,Yancheng Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nurr1 antibody [447C2a]

Western blot - Anti-Nurr1 antibody [447C2a] (AB54366)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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