AB227260

Anti-Nurr1 antibody

Name: Anti-Nurr1 antibody (ab227260) | Abcam
Brand: Abcam Limited
SKU: AB227260
Price: 525 USD
Availability: InStock

(1 Review)

(1 Publication)

Rabbit Polyclonal Nurr1 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NR4A2.

View Alternative Names

NOT, NURR1, TINUR, NR4A2, Nuclear receptor subfamily 4 group A member 2, Immediate-early response protein NOT, Orphan nuclear receptor NURR1, Transcriptionally-inducible nuclear receptor

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Nurr1 antibody (AB227260)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Nurr1 antibody (AB227260)

4% paraformaldehyde-fixed SK-N-SH (human neuroblastoma cell line) cells stained for Nurr1 (green) using ab227260 at 1/500 dilution in ICC/IF.

Red : Phalloidin, a cytoskeleton marker, at 1/50 dilution.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Nurr1 antibody (AB227260)

Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Nurr1 (green) using ab227260 at 1/500 dilution in ICC/IF.

Alpha-tubulin filaments were labeled with an alhpa-tubulin antibody at 1/2000 dilution (Red).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human NR4A2. The exact immunogen used to generate this antibody is proprietary information.

P43354

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nuclear receptor-related 1 protein (Nurr1) is a transcription factor weighing approximately 66 kDa. Scientists also know Nurr1 as NR4A2 and it belongs to the NR4A nuclear receptor family. Nurr1 is mainly expressed in the central nervous system where it plays a significant role in neuronal development and maintenance. Despite lacking a defined ligand-binding activity Nurr1 regulates gene expression directly by binding to DNA.

Biological function summary

Nurr1 functions as a critical regulator of dopaminergic neurons in the brain. It is vital for the survival and differentiation of these neurons which are important for motor control and behavioral responses. Nurr1 partners with other transcription factors often functioning in complexes that modulate brain-derived neurotrophic factor (BDNF) signaling. It influences the transcription of genes involved in inflammatory responses and neuroprotection reflecting its importance in maintaining neural health.

Pathways

Nurr1 integrates into key biological processes involving dopaminergic signaling and neuroinflammation. Nurr1 directly participates in the Wnt signaling pathway which plays a role in neuronal development and synaptic function. It also interacts with proteins such as Pitx3 and LMX1B within these pathways contributing to the regulation of dopaminergic neuron function and survival ultimately affecting broader central nervous system activity.

Nurr1 has strong associations with neurological conditions such as Parkinson’s disease and schizophrenia. Its impaired function or altered expression can lead to dopaminergic neuron degeneration a hallmark of Parkinson's disease. Research indicates Nurr1's interaction with parkin a protein implicated in the disease's pathology further emphasizes its importance. In schizophrenia altered Nurr1 activity affects synaptic regulation and cognitive function potentially relating to disrupted dopaminergic and BDNF pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development (PubMed : 15716272, PubMed : 17184956). It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons (By similarity).

See full target information NR4A2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancers 16: PubMed38339348

2024

Triple-Negative Breast Cancer Intrinsic FTSJ1 Favors Tumor Progression and Attenuates CD8+ T Cell Infiltration.

Applications

Unspecified application

Species

Unspecified reactive species

Yangqing Sun,Qingqing Liu,Shangwei Zhong,Rui Wei,Jun-Li Luo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nurr1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Nurr1 antibody (AB227260)

Immunocytochemistry/ Immunofluorescence - Anti-Nurr1 antibody (AB227260)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise