AB137230

Anti-NUSAP antibody

Name: Anti-NUSAP antibody (ab137230) | Abcam
Brand: Abcam Limited
SKU: AB137230
Price: 535 USD
Availability: InStock
Rating: 3 (2 reviews)

(2 Reviews)

(6 Publications)

Rabbit Polyclonal NUSAP antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human NUSAP1.

View Alternative Names

ANKT, BM-037, PRO0310, NUSAP1, Nucleolar and spindle-associated protein 1, NuSAP

1 Images

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Western blot - Anti-NUSAP antibody (AB137230)

All lanes:

Western blot - Anti-NUSAP antibody (ab137230) at 1/500 dilution

All lanes:

HT29 cell lysate at 30 µg

Predicted band size: 49 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human NUSAP1. The exact immunogen used to generate this antibody is proprietary information.

Q9BXS6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NUSAP1 also known as nucleolar spindle-associated protein 1 plays key role in cell division particularly in mitosis. It is located widely in the cell nucleus and gains its prominence during mitotic spindle assembly. With a molecular mass of about 55 kDa NUSAP1 is predominantly expressed in actively proliferating cells and has heightened expression in tissues with rapid cell division such as the thymus and bone marrow.

Biological function summary

NUSAP1 is significant in stabilizing microtubules and interacts with proteins like TPX2 to facilitate spindle formation. It binds to microtubules and augments their cross-linking and bundling influencing spindle dynamics. NUSAP1 does not work in isolation; it is a part of a larger complex that ensures proper chromosome segregation which is essential for accurate cell division.

Pathways

NUSAP1 is integral to the mitotic spindle assembly checkpoint an important part of the cell cycle. It associates with pathways such as the p53 signaling pathway which regulates cell division and growth. Within these pathways NUSAP1 interacts with proteins like AURKA and PLK1 marking its importance in maintaining DNA integrity and controlling mitotic events.

NUSAP1 has links to cancer especially in cases where cell proliferation is unregulated. Overexpression of NUSAP1 can lead to tumor progression making it a potential marker for cancer prognosis. Additionally disruptions in its function may tie to cell cycle disorders often connecting to proteins like MAD2L1 which also play roles in spindle checkpoint activities.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Microtubule-associated protein with the capacity to bundle and stabilize microtubules (By similarity). May associate with chromosomes and promote the organization of mitotic spindle microtubules around them.

See full target information NUSAP1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Life science alliance 6: PubMed36316035

2022

CRISPRi screening reveals regulators of tau pathology shared between exosomal and vesicle-free tau.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Carlos Polanco,Yevhen Akimov,Avinash Fernandes,Adam Briner,Gabriel Rhys Hand,Marloes van Roijen,Giuseppe Balistreri,Jürgen Götz

Molecular medicine reports 25: PubMed35485274

2022

Transcription factor HOXB2 upregulates NUSAP1 to promote the proliferation, invasion and migration of nephroblastoma cells via the PI3K/Akt signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Luo,Shasha Feng,Tianliang Li,Jun Wang,Zhaoyang Qi,Yi Zhao,Bo Hu

Scientific reports 12:7003 PubMed35487972

2022

Increased NUSAP1 expression is associated with lymph node metastasis and survival prognosis in bladder urothelial carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Hou,Zhenquan Lu,Xianhua Liu,Bingfeng Luo,Genyi Qu,Yong Xu,Cheng Tang

Gene 747:144657 PubMed32298762

2020

Overexpression of NuSAP1 is predictive of an unfavourable prognosis and promotes proliferation and invasion of triple-negative breast cancer cells via the Wnt/β-catenin/EMT signalling axis.

Applications

Unspecified application

Species

Unspecified reactive species

Li Sun,Changlong Shi,Shaozhuang Liu,Enchong Zhang,Long Yan,Ce Ji,Yi Zhao

OncoTargets and therapy 13:813-825 PubMed32099387

2020

Nucleolar and Spindle Associated Protein 1 (NUSAP1) Promotes Bladder Cancer Progression Through the TGF-β Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shun Gao,Hubin Yin,Hang Tong,Kai Zhan,Guang Yang,Mohammad Arman Hossain,Tinghao Li,Xin Gou,Weiyang He

The Journal of investigative dermatology 137:1682-1691 PubMed28545780

2017

Requirement of Zinc Transporter SLC39A7/ZIP7 for Dermal Development to Fine-Tune Endoplasmic Reticulum Function by Regulating Protein Disulfide Isomerase.

Applications

Unspecified application

Species

Unspecified reactive species

Bum-Ho Bin,Jinhyuk Bhin,Juyeon Seo,Se-Young Kim,Eunyoung Lee,Kyuhee Park,Dong-Hwa Choi,Teruhisa Takagishi,Takafumi Hara,Daehee Hwang,Haruhiko Koseki,Yoshinobu Asada,Shinji Shimoda,Kenji Mishima,Toshiyuki Fukada

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NUSAP antibody

Western blot - Anti-NUSAP antibody (AB137230)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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