Rabbit Polyclonal NUSAP antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human NUSAP1.
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- Scientific reports 12:70032022Increased NUSAP1 expression is associated with lymph node metastasis and survival prognosis in bladder urothelial carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian Hou,Zhenquan Lu,Xianhua Liu,Bingfeng Luo,Genyi Qu,Yong Xu,Cheng TangPubMed 35487972
- Gene 747:1446572020Overexpression of NuSAP1 is predictive of an unfavourable prognosis and promotes proliferation and invasion of triple-negative breast cancer cells via the Wnt/β-catenin/EMT signalling axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Sun et. al.PubMed 32298762
- OncoTargets and therapy 13:813-8252020Nucleolar and Spindle Associated Protein 1 (NUSAP1) Promotes Bladder Cancer Progression Through the TGF-β Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShun Gao et. al.PubMed 32099387
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human NUSAP1. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BXS6
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
Associated Products
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Target data
Function
Microtubule-associated protein with the capacity to bundle and stabilize microtubules (By similarity). May associate with chromosomes and promote the organization of mitotic spindle microtubules around them.
Alternative names
ANKT, BM-037, PRO0310, NUSAP1, Nucleolar and spindle-associated protein 1, NuSAP
Recommended products
Rabbit Polyclonal NUSAP antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human NUSAP1.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human NUSAP1. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BXS6
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NUSAP1 also known as nucleolar spindle-associated protein 1 plays key role in cell division particularly in mitosis. It is located widely in the cell nucleus and gains its prominence during mitotic spindle assembly. With a molecular mass of about 55 kDa NUSAP1 is predominantly expressed in actively proliferating cells and has heightened expression in tissues with rapid cell division such as the thymus and bone marrow.
- Biological function summary
NUSAP1 is significant in stabilizing microtubules and interacts with proteins like TPX2 to facilitate spindle formation. It binds to microtubules and augments their cross-linking and bundling influencing spindle dynamics. NUSAP1 does not work in isolation; it is a part of a larger complex that ensures proper chromosome segregation which is essential for accurate cell division.
- Pathways
NUSAP1 is integral to the mitotic spindle assembly checkpoint an important part of the cell cycle. It associates with pathways such as the p53 signaling pathway which regulates cell division and growth. Within these pathways NUSAP1 interacts with proteins like AURKA and PLK1 marking its importance in maintaining DNA integrity and controlling mitotic events.
- Associated diseases and disorders
NUSAP1 has links to cancer especially in cases where cell proliferation is unregulated. Overexpression of NUSAP1 can lead to tumor progression making it a potential marker for cancer prognosis. Additionally disruptions in its function may tie to cell cycle disorders often connecting to proteins like MAD2L1 which also play roles in spindle checkpoint activities.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-NUSAP antibody (ab137230)
All lanes: Western blot - Anti-NUSAP antibody (ab137230) at 1/500 dilution
All lanes: HT29 cell lysate at 30 µg
Predicted band size: 49 kDa
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