AB169083

Anti-NUSAP antibody

Name: Anti-NUSAP antibody (ab169083) | Abcam
Brand: Abcam Limited
SKU: AB169083

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(4 Publications)

Mouse Polyclonal NUSAP antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NUSAP1 aa 200 to C-terminus.

View Alternative Names

ANKT, BM-037, PRO0310, NUSAP1, Nucleolar and spindle-associated protein 1, NuSAP

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-NUSAP antibody (AB169083)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NUSAP antibody (AB169083)

Immunofluorescent analysis of HeLa cells labeling NuSAP with ab169083 at 10μg/ml.

Unknown

Western blot - Anti-NUSAP antibody (AB169083)

All lanes:

Western blot - Anti-NUSAP antibody (ab169083) at 1 µg/mL

Lane 1:

NUSAP1-transfected 293T cell lysate at 15 µL

Lane 2:

Non-transfected 293T cell lysate at 15 µL

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 49 kDa

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Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human NUSAP1 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9BXS6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NUSAP1 also known as nucleolar spindle-associated protein 1 plays key role in cell division particularly in mitosis. It is located widely in the cell nucleus and gains its prominence during mitotic spindle assembly. With a molecular mass of about 55 kDa NUSAP1 is predominantly expressed in actively proliferating cells and has heightened expression in tissues with rapid cell division such as the thymus and bone marrow.

Biological function summary

NUSAP1 is significant in stabilizing microtubules and interacts with proteins like TPX2 to facilitate spindle formation. It binds to microtubules and augments their cross-linking and bundling influencing spindle dynamics. NUSAP1 does not work in isolation; it is a part of a larger complex that ensures proper chromosome segregation which is essential for accurate cell division.

Pathways

NUSAP1 is integral to the mitotic spindle assembly checkpoint an important part of the cell cycle. It associates with pathways such as the p53 signaling pathway which regulates cell division and growth. Within these pathways NUSAP1 interacts with proteins like AURKA and PLK1 marking its importance in maintaining DNA integrity and controlling mitotic events.

NUSAP1 has links to cancer especially in cases where cell proliferation is unregulated. Overexpression of NUSAP1 can lead to tumor progression making it a potential marker for cancer prognosis. Additionally disruptions in its function may tie to cell cycle disorders often connecting to proteins like MAD2L1 which also play roles in spindle checkpoint activities.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Microtubule-associated protein with the capacity to bundle and stabilize microtubules (By similarity). May associate with chromosomes and promote the organization of mitotic spindle microtubules around them.

See full target information NUSAP1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Open medicine (Warsaw, Poland) 16:1083-1089 PubMed34322597

2021

Hypoxia stimulates the migration and invasion of osteosarcoma via up-regulating the NUSAP1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Zhang,Jingtao Song,Xu Xin,Donghong Sun,Huiting Huang,Yang Chen,Tao Zhang,Yiming Zhang

Experimental and therapeutic medicine 20:1145-1152 PubMed32765662

2020

Prazosin inhibits the proliferation, migration and invasion, but promotes the apoptosis of U251 and U87 cells via the PI3K/AKT/mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhang,Jiye Fan

Technology in cancer research & treatment 19:1533033820905823 PubMed32233835

2020

Knockdown of lncRNA OGFRP1 Inhibits Proliferation and Invasion of JEG-3 Cells Via AKT/mTOR Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Meng,Haiyan Xue

Aging 12:2156-2168 PubMed32018225

2020

Knockdown of P3H4 inhibits proliferation and invasion of bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Hao,Kun Pang,Hui Pang,Junjie Zhang,Zhiguo Zhang,Houguang He,Rongsheng Zhou,Zhenduo Shi,Conghui Han

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NUSAP antibody

Immunocytochemistry/ Immunofluorescence - Anti-NUSAP antibody (AB169083)

Western blot - Anti-NUSAP antibody (AB169083)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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