AB247043

Anti-NUSAP antibody

Name: Anti-NUSAP antibody (ab247043) | Abcam
Brand: Abcam Limited
SKU: AB247043
Price: 655 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal NUSAP antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NUSAP1 aa 1-200.

View Alternative Names

ANKT, BM-037, PRO0310, NUSAP1, Nucleolar and spindle-associated protein 1, NuSAP

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUSAP antibody (AB247043)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUSAP antibody (AB247043)

Paraffin-embedded human testis tissue stained for NUSAP using ab247043 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUSAP antibody (AB247043)

Paraffin-embedded human cerebral cortex tissue stained for NUSAP using ab247043 at 1/500 dilution in immunohistochemical analysis. No positivity in neuronal cells as expected.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUSAP antibody (AB247043)

Paraffin-embedded human bone marrow tissue stained for NUSAP using ab247043 at 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-NUSAP antibody (AB247043)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NUSAP antibody (AB247043)

PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling NUSAP using ab247043 at 4 μg/ml (green) in ICC/IF.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUSAP antibody (AB247043)

Paraffin-embedded human lymph node tissue stained for NUSAP using ab247043 at 1/500 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human NUSAP1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q9BXS6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NUSAP1 also known as nucleolar spindle-associated protein 1 plays key role in cell division particularly in mitosis. It is located widely in the cell nucleus and gains its prominence during mitotic spindle assembly. With a molecular mass of about 55 kDa NUSAP1 is predominantly expressed in actively proliferating cells and has heightened expression in tissues with rapid cell division such as the thymus and bone marrow.

Biological function summary

NUSAP1 is significant in stabilizing microtubules and interacts with proteins like TPX2 to facilitate spindle formation. It binds to microtubules and augments their cross-linking and bundling influencing spindle dynamics. NUSAP1 does not work in isolation; it is a part of a larger complex that ensures proper chromosome segregation which is essential for accurate cell division.

Pathways

NUSAP1 is integral to the mitotic spindle assembly checkpoint an important part of the cell cycle. It associates with pathways such as the p53 signaling pathway which regulates cell division and growth. Within these pathways NUSAP1 interacts with proteins like AURKA and PLK1 marking its importance in maintaining DNA integrity and controlling mitotic events.

NUSAP1 has links to cancer especially in cases where cell proliferation is unregulated. Overexpression of NUSAP1 can lead to tumor progression making it a potential marker for cancer prognosis. Additionally disruptions in its function may tie to cell cycle disorders often connecting to proteins like MAD2L1 which also play roles in spindle checkpoint activities.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Microtubule-associated protein with the capacity to bundle and stabilize microtubules (By similarity). May associate with chromosomes and promote the organization of mitotic spindle microtubules around them.

See full target information NUSAP1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 22:142 PubMed38383392

2024

TFEB activation triggers pexophagy for functional adaptation during oxidative stress under calcium deficient-conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Laxman Manandhar,Raghbendra Kumar Dutta,Pradeep Devkota,Arun Chhetri,Xiaofan Wei,Channy Park,Hyug Moo Kwon,Raekil Park

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NUSAP antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUSAP antibody (AB247043)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUSAP antibody (AB247043)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUSAP antibody (AB247043)

Immunocytochemistry/ Immunofluorescence - Anti-NUSAP antibody (AB247043)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUSAP antibody (AB247043)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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