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AB122649

Anti-NUT antibody

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(3 Publications)

Anti-NUT antibody (ab122649) is a rabbit polyclonal antibody detecting NUT in IHC-P. Suitable for Human.

View Alternative Names

C15orf55, NUT, NUTM1, NUT family member 1, Nuclear protein in testis

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody (AB122649)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody (AB122649)

ab122649 (1/3000), staining NUT in paraffin embedded Human testis tissue by Immunohistochemistry.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody (AB122649)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody (AB122649)

ab122649 (1/3000), showing negative staining NUT in paraffin embedded Human skeletal muscle tissue by Immunohistochemistry.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody (AB122649)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody (AB122649)

ab122649 (1/3000), showing negative staining NUT in paraffin embedded Human liver tissue by Immunohistochemistry.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody (AB122649)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody (AB122649)

ab122649 (1/3000), showing negative staining NUT in paraffin embedded Human endometrium tissue by Immunohistochemistry.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human NUTM1 aa 900-1000. The exact immunogen used to generate this antibody is proprietary information.

Q86Y26

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Product details

What is this antibody validated in?
Anti-NUT antibody (ab122649) is a rabbit polyclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human samples.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NUT protein known also as Nuclear Protein in Testis is a highly specialized protein with a molecular weight of approximately 112 kDa. Its expression is mostly restricted to post-meiotic germ cells in the testes but aberrant expression occurs in some cancer types. NUT is mainly localized in the nucleus and exhibits involvement in transcriptional regulation due to its structure and function in gene expression processes. It engages in forming fusion proteins notably through translocations which has particular relevance in oncogenesis.
Biological function summary

NUT plays an important role in the regulation of chromatin structure and gene expression. It usually forms a part of the large complex involving histone acetyltransferase which contributes to chromatin remodeling. The protein's interaction with various transcriptional coactivators suggests its function in facilitating access to DNA for transcription machinery. In normal cellular function NUT is limited in expression and activity asserting its importance in specific developmental processes.

Pathways

NUT engages in significant modulation of chromatin remodeling and transcriptional pathways including the BRD4-NUT fusion pathway in cancer biology. This pathway involves the BRD4 protein which forms a fusion with NUT through chromosomal translocations altering normal epigenetic regulation and driving abnormally high transcription of growth-promoting genes. The BRD-NUT fusion therefore creates a powerful oncogenic driver within the transcriptional machinery and chromatin modification pathways.

Aberrations in NUT are closely linked to aggressive forms of cancer such as NUT midline carcinoma. This cancer type arises from the NUT fusion with other proteins like BRD4 or BRD3 resulting in altered gene expression leading to unchecked cell proliferation. The disorders related to NUT reexpression highlight its pathological importance and targeting the BRD-NUT fusion protein has become a focus for therapeutic intervention in treating related malignancies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in the regulation of proliferation. Regulates TERT expression by modulating SP1 binding to TERT promoter binding sites.
See full target information NUTM1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Translational cancer research 11:1362-1371 PubMed35706822

2022

Long non-coding RNA (LncRNA) CHROMR promotes the expression of the gene by adsorbing hsa-miR-1299 to obtain drug resistance in diffuse large B lymphoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mingyue Wang,Zhichao Miao,Hong Cen,Jiaxu He,Changyuan Wei

Archives of pathology & laboratory medicine 146:735-741 PubMed34525172

2021

CIC-NUTM1 Sarcomas Affecting the Spine.

Applications

Unspecified application

Species

Unspecified reactive species

Shaomin Yang,LiLi Liu,Yu Yan,Liang Jiang,Songbo Han,Danhua Shen,Bo Zhang

BioMed research international 2020:9791208 PubMed32149149

2020

Clinicopathological Analysis of Five Cases of NUT Midline Carcinoma, including One with the Gingiva.

Applications

Unspecified application

Species

Unspecified reactive species

Luting Zhou,Xiang Yong,Jun Zhou,Jiankun Xu,Chaofu Wang
View all publications
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