Rabbit Recombinant Monoclonal NUT antibody. Carrier free. Suitable for IHC-P and reacts with Human, Transfected cell line - Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody [EPR28221-46] - BSA and Azide free (AB315461), expandable thumbnail](https://content.abcam.com/products/images/nut-antibody-epr28221-46-bsa-and-azide-free-ab315461--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425198.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody [EPR28221-46] - BSA and Azide free (AB315461)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody [EPR28221-46] - BSA and Azide free (AB315461), expandable thumbnail](https://content.abcam.com/products/images/nut-antibody-epr28221-46-bsa-and-azide-free-ab315461--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425197.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody [EPR28221-46] - BSA and Azide free (AB315461)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody [EPR28221-46] - BSA and Azide free (AB315461), expandable thumbnail](https://content.abcam.com/products/images/nut-antibody-epr28221-46-bsa-and-azide-free-ab315461--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425196.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody [EPR28221-46] - BSA and Azide free (AB315461)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody [EPR28221-46] - BSA and Azide free (AB315461), expandable thumbnail](https://content.abcam.com/products/images/nut-antibody-epr28221-46-bsa-and-azide-free-ab315461--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425195.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody [EPR28221-46] - BSA and Azide free (AB315461)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody [EPR28221-46] - BSA and Azide free (AB315461), expandable thumbnail](https://content.abcam.com/products/images/nut-antibody-epr28221-46-bsa-and-azide-free-ab315461--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425194.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody [EPR28221-46] - BSA and Azide free (AB315461)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | IP | Flow Cyt (Intra) | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Transfected cell line - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Transfected cell line - Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell line - Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell line - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell line - Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell line - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Plays a role in the regulation of proliferation. Regulates TERT expression by modulating SP1 binding to TERT promoter binding sites.
Alternative names
C15orf55, NUT, NUTM1, NUT family member 1, Nuclear protein in testis
Recommended products
Rabbit Recombinant Monoclonal NUT antibody. Carrier free. Suitable for IHC-P and reacts with Human, Transfected cell line - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR28221-46
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab315461 is the carrier-free version of Anti-NUT antibody [EPR28221-46] ab315460.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The NUT protein known also as Nuclear Protein in Testis is a highly specialized protein with a molecular weight of approximately 112 kDa. Its expression is mostly restricted to post-meiotic germ cells in the testes but aberrant expression occurs in some cancer types. NUT is mainly localized in the nucleus and exhibits involvement in transcriptional regulation due to its structure and function in gene expression processes. It engages in forming fusion proteins notably through translocations which has particular relevance in oncogenesis.
- Biological function summary
NUT plays an important role in the regulation of chromatin structure and gene expression. It usually forms a part of the large complex involving histone acetyltransferase which contributes to chromatin remodeling. The protein's interaction with various transcriptional coactivators suggests its function in facilitating access to DNA for transcription machinery. In normal cellular function NUT is limited in expression and activity asserting its importance in specific developmental processes.
- Pathways
NUT engages in significant modulation of chromatin remodeling and transcriptional pathways including the BRD4-NUT fusion pathway in cancer biology. This pathway involves the BRD4 protein which forms a fusion with NUT through chromosomal translocations altering normal epigenetic regulation and driving abnormally high transcription of growth-promoting genes. The BRD-NUT fusion therefore creates a powerful oncogenic driver within the transcriptional machinery and chromatin modification pathways.
- Associated diseases and disorders
Aberrations in NUT are closely linked to aggressive forms of cancer such as NUT midline carcinoma. This cancer type arises from the NUT fusion with other proteins like BRD4 or BRD3 resulting in altered gene expression leading to unchecked cell proliferation. The disorders related to NUT reexpression highlight its pathological importance and targeting the BRD-NUT fusion protein has become a focus for therapeutic intervention in treating related malignancies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody [EPR28221-46] - BSA and Azide free (ab315461), expandable thumbnail](https://content.abcam.com/products/images/nut-antibody-epr28221-46-bsa-and-azide-free-ab315461--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425198.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody [EPR28221-46] - BSA and Azide free (ab315461)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody [EPR28221-46] - BSA and Azide free (ab315461)
This data was developed using Anti-NUT antibody [EPR28221-46] ab315460, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Tissue Microarray tissue labeling NUT with Anti-NUT antibody [EPR28221-46] ab315460, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Table of the human normal TMA tissue depending on the staining. The section was incubated with Anti-NUT antibody [EPR28221-46] ab315460 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody [EPR28221-46] - BSA and Azide free (ab315461), expandable thumbnail](https://content.abcam.com/products/images/nut-antibody-epr28221-46-bsa-and-azide-free-ab315461--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425197.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody [EPR28221-46] - BSA and Azide free (ab315461)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody [EPR28221-46] - BSA and Azide free (ab315461)
This data was developed using Anti-NUT antibody [EPR28221-46] ab315460, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling NUT with Anti-NUT antibody [EPR28221-46] ab315460 at 1/5000 (0.103 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Negative control: no staining on human cerebrum. The section was incubated with Anti-NUT antibody [EPR28221-46] ab315460 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody [EPR28221-46] - BSA and Azide free (ab315461), expandable thumbnail](https://content.abcam.com/products/images/nut-antibody-epr28221-46-bsa-and-azide-free-ab315461--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425196.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody [EPR28221-46] - BSA and Azide free (ab315461)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody [EPR28221-46] - BSA and Azide free (ab315461)
This data was developed using Anti-NUT antibody [EPR28221-46] ab315460, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling NUT with Anti-NUT antibody [EPR28221-46] ab315460 at 1/5000 (0.103 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Negative control: no staining on human liver. The section was incubated with Anti-NUT antibody [EPR28221-46] ab315460 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody [EPR28221-46] - BSA and Azide free (ab315461), expandable thumbnail](https://content.abcam.com/products/images/nut-antibody-epr28221-46-bsa-and-azide-free-ab315461--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425195.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody [EPR28221-46] - BSA and Azide free (ab315461)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody [EPR28221-46] - BSA and Azide free (ab315461)
This data was developed using Anti-NUT antibody [EPR28221-46] ab315460, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Panel A HEK-293T cells transfected with a human BRD4-NUT expression vector containing a his tag. Panel B HEK-293T cells transfected with empty vector containing a his tag tissue labeling NUT with Anti-NUT antibody [EPR28221-46] ab315460 at 1/5000 (0.103 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on (A) HEK-293T transfected with a human BRD4-NUT expression vector, no staining on (B) HEK-293T transfected with empty vector. The section was incubated with Anti-NUT antibody [EPR28221-46] ab315460 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody [EPR28221-46] - BSA and Azide free (ab315461), expandable thumbnail](https://content.abcam.com/products/images/nut-antibody-epr28221-46-bsa-and-azide-free-ab315461--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425194.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody [EPR28221-46] - BSA and Azide free (ab315461)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUT antibody [EPR28221-46] - BSA and Azide free (ab315461)
This data was developed using Anti-NUT antibody [EPR28221-46] ab315460, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling NUT with Anti-NUT antibody [EPR28221-46] ab315460 at 1/5000 (0.103 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on human testis. The section was incubated with Anti-NUT antibody [EPR28221-46] ab315460 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com