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AB129160

Anti-NXF1 antibody [EPR8009]

5

(2 Reviews)

|

(7 Publications)

Rabbit Recombinant Monoclonal NXF1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 7 publications.

View Alternative Names

TAP, NXF1, Nuclear RNA export factor 1, Tip-associated protein, Tip-associating protein, mRNA export factor TAP

7 Images
Flow Cytometry (Intracellular) - Anti-NXF1 antibody [EPR8009] (AB129160)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-NXF1 antibody [EPR8009] (AB129160)

Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling NXF1 with purified ab129160 at 1/20 dilution (5 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunocytochemistry/ Immunofluorescence - Anti-NXF1 antibody [EPR8009] (AB129160)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NXF1 antibody [EPR8009] (AB129160)

ab129160 at 1/250 dilution staining NXF1 in HepG2 cells by immunofluorescence.

Immunocytochemistry/ Immunofluorescence - Anti-NXF1 antibody [EPR8009] (AB129160)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NXF1 antibody [EPR8009] (AB129160)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling NXF1 with purified ab129160 at 1/200 dilution (0.62 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Immunoprecipitation - Anti-NXF1 antibody [EPR8009] (AB129160)
  • IP

Lab

Immunoprecipitation - Anti-NXF1 antibody [EPR8009] (AB129160)

ab129160 at 1/20 immunoprecipitating NXF1 in HeLa (human cervix adenocarcinoma) whole cell lysate observed at 70 KDa (Lanes 1 and 2).

Lane 1 (input) : HeLa whole cell lysate, 10μg
Lane 2 (+) : ab129160 + HeLa whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab129160 in HeLa whole cell lysate

For western blotting, ab129160 at 1/500 dilution and ab131366 VeriBlot for IP (HRP) at 1/1000 were used.

Blocking and Diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-NXF1 antibody [EPR8009] (ab129160)

Predicted band size: 70 kDa

false

Exposure time: 1s

Immunoprecipitation - Anti-NXF1 antibody [EPR8009] (AB129160)
  • IP

Lab

Immunoprecipitation - Anti-NXF1 antibody [EPR8009] (AB129160)

ab129160 at 1/20 immunoprecipitating NXF1 in HepG2 (human hepatocellular carcinoma) whole cell lysate observed at 70 KDa (Lanes 1 and 2).

Lane 1 (input) : HepG2 whole cell lysate, 10μg
Lane 2 (+) : ab129160 + HepG2 whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab129160 in HepG2 whole cell lysate

For western blotting, ab129160 at 1/500 dilution and ab131366 VeriBlot for IP (HRP) at 1/1000 were used.

Blocking and Diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-NXF1 antibody [EPR8009] (ab129160)

Predicted band size: 70 kDa

false

Exposure time: 1s

Western blot - Anti-NXF1 antibody [EPR8009] (AB129160)
  • WB

Lab

Western blot - Anti-NXF1 antibody [EPR8009] (AB129160)

All lanes:

Western blot - Anti-NXF1 antibody [EPR8009] (ab129160) at 1/2000 dilution

All lanes:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 70 kDa

Observed band size: 70 kDa

false

Western blot - Anti-NXF1 antibody [EPR8009] (AB129160)
  • WB

Unknown

Western blot - Anti-NXF1 antibody [EPR8009] (AB129160)

All lanes:

Western blot - Anti-NXF1 antibody [EPR8009] (ab129160) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 70 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8009

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IP, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NXF1 also known as TAP functions as an mRNA nuclear export factor. It plays a critical role in transporting mRNA from the nucleus to the cytoplasm. NXF1 protein mass is approximately 70 kDa. Researchers primarily observe its expression in various human tissues with higher expression in the liver and brain. The NXF1 protein interacts with nuclear pore complexes to facilitate mRNA transport making it important in gene expression regulation.
Biological function summary

This mRNA transport factor ensures correct mRNA localization and effective translation into proteins. NXF1 acts part of a multiprotein complex where it interacts with NXT1 to guide mature mRNA to the nuclear pore for export. This action is central for maintaining cellular protein homeostasis and enables proper cellular responses to different stimuli. Disruptions in NXF1 function can cause interruptions in the mRNA export process impacting protein synthesis.

Pathways

NXF1 takes part in essential cellular processes related to nucleocytoplasmic transport. It operates within the mRNA export pathway ensuring mRNAs navigate from the nucleus to the cytoplasm. NXF1 closely works with other export factors such as NUP98 in this pathway. Additionally it associates with the spliceosome helping to coordinate the export of correctly spliced mRNA which is important for efficient gene expression and cellular function.

NXF1 alterations have connections to certain types of cancer and neurological disorders. For instance malfunction in its mRNA export role can contribute to tumorigenesis as seen with breast cancer by misregulating gene expression. In neurological diseases like amyotrophic lateral sclerosis (ALS) defects in mRNA transport can impair neuronal function. Genetic disruptions impacting NXF1 dynamics often involve proteins like FUS which also participate in RNA metabolism and are linked to similar disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm (TAP/NFX1 pathway) (PubMed : 10924507). The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5 components of the TREX complex (PubMed : 18364396, PubMed : 19165146, PubMed : 9660949). ALYREF/THOC4-bound mRNA is thought to be transferred to the NXF1-NXT1 heterodimer for export (PubMed : 18364396, PubMed : 19165146, PubMed : 9660949). Also involved in nuclear export of m6A-containing mRNAs : interaction between SRSF3 and YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export (PubMed : 28984244).
See full target information NXF1
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 43:113610 PubMed38165804

2024

The FANCI/FANCD2 complex links DNA damage response to R-loop regulation through SRSF1-mediated mRNA export.

Applications

Unspecified application

Species

Unspecified reactive species

Anne Olazabal-Herrero,Boxue He,Youngho Kwon,Abhishek K Gupta,Arijit Dutta,Yuxin Huang,Prajwal Boddu,Zhuobin Liang,Fengshan Liang,Yaqun Teng,Li Lan,Xiaoyong Chen,Huadong Pei,Manoj M Pillai,Patrick Sung,Gary M Kupfer

Cell reports 40:111106 PubMed35858577

2022

Nuclear RNA binding regulates TDP-43 nuclear localization and passive nuclear export.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren Duan,Benjamin L Zaepfel,Vasilisa Aksenova,Mary Dasso,Jeffrey D Rothstein,Petr Kalab,Lindsey R Hayes

Frontiers in cell and developmental biology 9:809859 PubMed34970554

2021

Polyadenylated RNA and RNA-Binding Proteins Exhibit Unique Response to Hyperosmotic Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin L Zaepfel,Jeffrey D Rothstein

eLife 10: PubMed34110283

2021

T-cell receptor (TCR) signaling promotes the assembly of RanBP2/RanGAP1-SUMO1/Ubc9 nuclear pore subcomplex via PKC-θ-mediated phosphorylation of RanGAP1.

Applications

Unspecified application

Species

Unspecified reactive species

Yujiao He,Zhiguo Yang,Chen-Si Zhao,Zhihui Xiao,Yu Gong,Yun-Yi Li,Yiqi Chen,Yunting Du,Dianying Feng,Amnon Altman,Yingqiu Li

Cells 9: PubMed32403279

2020

Super-Resolution Localisation of Nuclear PI(4)P and Identification of Its Interacting Proteome.

Applications

Unspecified application

Species

Unspecified reactive species

Veronika Fáberová,Ilona Kalasová,Alžběta Krausová,Pavel Hozák

The Journal of cell biology 218:2962-2981 PubMed31375530

2019

Imaging within single NPCs reveals NXF1's role in mRNA export on the cytoplasmic side of the pore.

Applications

Unspecified application

Species

Unspecified reactive species

Rakefet Ben-Yishay,Amir Mor,Amit Shraga,Asaf Ashkenazy-Titelman,Noa Kinor,Avital Schwed-Gross,Avi Jacob,Noga Kozer,Pramod Kumar,Yuval Garini,Yaron Shav-Tal

eLife 6: PubMed28984244

2017

YTHDC1 mediates nuclear export of N-methyladenosine methylated mRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Ian A Roundtree,Guan-Zheng Luo,Zijie Zhang,Xiao Wang,Tao Zhou,Yiquang Cui,Jiahao Sha,Xingxu Huang,Laura Guerrero,Phil Xie,Emily He,Bin Shen,Chuan He
View all publications
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