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AB248319

Anti-NXF1 antibody [EPR8009] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal NXF1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

TAP, NXF1, Nuclear RNA export factor 1, Tip-associated protein, Tip-associating protein, mRNA export factor TAP

2 Images
Flow Cytometry (Intracellular) - Anti-NXF1 antibody [EPR8009] - BSA and Azide free (AB248319)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-NXF1 antibody [EPR8009] - BSA and Azide free (AB248319)

Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling NXF1 with purified ab129160 at 1/20 dilution (5 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129160)

Immunocytochemistry/ Immunofluorescence - Anti-NXF1 antibody [EPR8009] - BSA and Azide free (AB248319)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NXF1 antibody [EPR8009] - BSA and Azide free (AB248319)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling NXF1 with purified ab129160 at 1/200 dilution (0.62 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129160)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8009

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra), WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB202894

Anti-Aly/Ref antibody [EPR17942]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Aly/Ref antibody [EPR17942] (AB202894)

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Primary Antibodies

AB172634

Anti-SF3B1 antibody [EPR11986]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-SF3B1 antibody [EPR11986] (AB172634)

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Primary Antibodies

AB24609

Anti-Nuclear Pore Complex Proteins antibody [Mab414]

Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Pore Complex Proteins antibody [Mab414] (AB24609)

  • 4
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

ab248319 is the carrier-free version of ab129160.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NXF1 also known as TAP functions as an mRNA nuclear export factor. It plays a critical role in transporting mRNA from the nucleus to the cytoplasm. NXF1 protein mass is approximately 70 kDa. Researchers primarily observe its expression in various human tissues with higher expression in the liver and brain. The NXF1 protein interacts with nuclear pore complexes to facilitate mRNA transport making it important in gene expression regulation.
Biological function summary

This mRNA transport factor ensures correct mRNA localization and effective translation into proteins. NXF1 acts part of a multiprotein complex where it interacts with NXT1 to guide mature mRNA to the nuclear pore for export. This action is central for maintaining cellular protein homeostasis and enables proper cellular responses to different stimuli. Disruptions in NXF1 function can cause interruptions in the mRNA export process impacting protein synthesis.

Pathways

NXF1 takes part in essential cellular processes related to nucleocytoplasmic transport. It operates within the mRNA export pathway ensuring mRNAs navigate from the nucleus to the cytoplasm. NXF1 closely works with other export factors such as NUP98 in this pathway. Additionally it associates with the spliceosome helping to coordinate the export of correctly spliced mRNA which is important for efficient gene expression and cellular function.

NXF1 alterations have connections to certain types of cancer and neurological disorders. For instance malfunction in its mRNA export role can contribute to tumorigenesis as seen with breast cancer by misregulating gene expression. In neurological diseases like amyotrophic lateral sclerosis (ALS) defects in mRNA transport can impair neuronal function. Genetic disruptions impacting NXF1 dynamics often involve proteins like FUS which also participate in RNA metabolism and are linked to similar disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm (TAP/NFX1 pathway) (PubMed : 10924507). The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5 components of the TREX complex (PubMed : 18364396, PubMed : 19165146, PubMed : 9660949). ALYREF/THOC4-bound mRNA is thought to be transferred to the NXF1-NXT1 heterodimer for export (PubMed : 18364396, PubMed : 19165146, PubMed : 9660949). Also involved in nuclear export of m6A-containing mRNAs : interaction between SRSF3 and YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export (PubMed : 28984244).
See full target information NXF1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell proliferation 56:e13400 PubMed36642844

2023

Non-SMC condensin I complex subunit H participates in anti-programmed cell death-1 resistance of clear cell renal cell carcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Chen,Weiqiang Ruan,Chunhao Guo,Ke Chen,Le Li,Jihua Tian,Zhiquan Hu,Dan Peng,Xing Zeng
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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