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Rabbit Recombinant Monoclonal NXN/NRX antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples.

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Images

Western blot - Anti-NXN/NRX antibody [EPR15177] (AB191429), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-NXN/NRX antibody [EPR15177] (AB191429), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NXN/NRX antibody [EPR15177] (AB191429), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NXN/NRX antibody [EPR15177] (AB191429), expandable thumbnail
  • Western blot - Anti-NXN/NRX antibody [EPR15177] (AB191429), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Expected
Tested
Mouse
Tested
Expected
Tested
Tested
Rat
Tested
Expected
Expected
Expected

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/50 - 1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/50 - 1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Functions as a redox-dependent negative regulator of the Wnt signaling pathway, possibly by preventing ubiquitination of DVL3 by the BCR(KLHL12) complex. May also function as a transcriptional regulator act as a regulator of protein phosphatase 2A (PP2A) (By similarity).

Alternative names

NRX, NXN, Nucleoredoxin

Recommended products

Rabbit Recombinant Monoclonal NXN/NRX antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR15177
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

NXN also known as nucleoredoxin or NRX is a redox-regulating protein that plays a role in cellular signaling. This protein weighs approximately 49 kDa and is expressed in a wide range of tissues including heart liver and lungs. It’s known for its function in controlling redox states within cells. NXN acts within the cytoplasm where it interacts with other proteins to influence various signaling pathways. Given its importance scientists often study NXN to understand how cells manage oxidative stress and maintain equilibrium.

Biological function summary

NXN acts as a thioredoxin-like protein that regulates the Wnt signaling pathway through its interaction with disheveled proteins. This protein is often involved in cellular processes by managing the balance between oxidative and reductive states critical for normal cellular function. NXN exists in cells as part of larger protein assemblies which allows it to control multiple signaling hubs within the cell. This ability makes NXN integral to the modulation of cellular responses and adaptation to changes in environmental conditions.

Pathways

NXN is influential in the Wnt and JNK signaling pathways. In the Wnt signaling pathway NXN influences cellular fate decisions by regulating the availability of disheveled proteins. It also interacts with components of the JNK signaling pathway which is involved in controlling apoptosis and stress responses. Proteins such as β-catenin and JNK3 are affected by NXN's function highlighting its extensive reach in cellular mechanism and signaling control.

Associated diseases and disorders

NXN has links to cancer and neurodegenerative diseases. Dysregulation in NXN can lead to abnormal cell proliferation linking it to the development of tumors. Its involvement in oxidative stress also places it in connection with neurodegenerative disorders like Alzheimer's disease. In cancer its interaction with proteins like p53 highlights its role in maintaining genomic stability. In the context of neurodegeneration NXN's relationship with proteins such as β-amyloid illustrates its potential involvement in disease progression and pathology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-NXN/NRX antibody [EPR15177] (ab191429), expandable thumbnail

    Western blot - Anti-NXN/NRX antibody [EPR15177] (ab191429)

    All lanes: Western blot - Anti-NXN/NRX antibody [EPR15177] (ab191429) at 1/10000 dilution

    Lane 1: 293 lysate at 20 µg

    Lane 2: HepG2 lysate at 20 µg

    Lane 3: HeLa lysate at 20 µg

    Lane 4: SH-SY5Y lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa

    Observed band size: 48 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-NXN/NRX antibody [EPR15177] (ab191429), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-NXN/NRX antibody [EPR15177] (ab191429)

    Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling NXN/NRX with ab191429 at 1/250, Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 and DAPI staining (blue). The two negative controls: 1. Primary ab concentration (anti-NXN/NRX) is 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/400 dilution; 2. Primary ab concentration (anti-NXN/NRX) is 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/400 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NXN/NRX antibody [EPR15177] (ab191429), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NXN/NRX antibody [EPR15177] (ab191429)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue labeling NXN/NRX with ab191429 at 1/100 with prediluted HRP Polymer for Rabbit IgG as secondary antibody. Negative control: Using PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NXN/NRX antibody [EPR15177] (ab191429), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NXN/NRX antibody [EPR15177] (ab191429)

    Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder tissue labeling NXN/NRX with ab191429 at 1/100 with prediluted HRP Polymer for Rabbit IgG as secondary antibody. Negative control: Using PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-NXN/NRX antibody [EPR15177] (ab191429), expandable thumbnail

    Western blot - Anti-NXN/NRX antibody [EPR15177] (ab191429)

    All lanes: Western blot - Anti-NXN/NRX antibody [EPR15177] (ab191429) at 1/10000 dilution

    Lane 1: Mouse testis lysate at 10 µg

    Lane 2: Rat testis lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa

    Observed band size: 48 kDa

  • Flow Cytometry (Intracellular) - Anti-NXN/NRX antibody [EPR15177] (ab191429), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-NXN/NRX antibody [EPR15177] (ab191429)

    Intracellular Flow Cytometry analysis of NIH/3T3 (mouse embryo) labelling NXN/NRXwith purified ab191429 at 1/1000 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Alexa Fluor® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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