Rabbit Recombinant Monoclonal NXN/NRX antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
---|---|---|---|---|
Human | Tested | Tested | Expected | Tested |
Mouse | Tested | Expected | Tested | Tested |
Rat | Tested | Expected | Expected | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Functions as a redox-dependent negative regulator of the Wnt signaling pathway, possibly by preventing ubiquitination of DVL3 by the BCR(KLHL12) complex. May also function as a transcriptional regulator act as a regulator of protein phosphatase 2A (PP2A) (By similarity).
NRX, NXN, Nucleoredoxin
Rabbit Recombinant Monoclonal NXN/NRX antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
NXN also known as nucleoredoxin or NRX is a redox-regulating protein that plays a role in cellular signaling. This protein weighs approximately 49 kDa and is expressed in a wide range of tissues including heart liver and lungs. It’s known for its function in controlling redox states within cells. NXN acts within the cytoplasm where it interacts with other proteins to influence various signaling pathways. Given its importance scientists often study NXN to understand how cells manage oxidative stress and maintain equilibrium.
NXN acts as a thioredoxin-like protein that regulates the Wnt signaling pathway through its interaction with disheveled proteins. This protein is often involved in cellular processes by managing the balance between oxidative and reductive states critical for normal cellular function. NXN exists in cells as part of larger protein assemblies which allows it to control multiple signaling hubs within the cell. This ability makes NXN integral to the modulation of cellular responses and adaptation to changes in environmental conditions.
NXN is influential in the Wnt and JNK signaling pathways. In the Wnt signaling pathway NXN influences cellular fate decisions by regulating the availability of disheveled proteins. It also interacts with components of the JNK signaling pathway which is involved in controlling apoptosis and stress responses. Proteins such as β-catenin and JNK3 are affected by NXN's function highlighting its extensive reach in cellular mechanism and signaling control.
NXN has links to cancer and neurodegenerative diseases. Dysregulation in NXN can lead to abnormal cell proliferation linking it to the development of tumors. Its involvement in oxidative stress also places it in connection with neurodegenerative disorders like Alzheimer's disease. In cancer its interaction with proteins like p53 highlights its role in maintaining genomic stability. In the context of neurodegeneration NXN's relationship with proteins such as β-amyloid illustrates its potential involvement in disease progression and pathology.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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All lanes: Western blot - Anti-NXN/NRX antibody [EPR15177] (ab191429) at 1/10000 dilution
Lane 1: 293 lysate at 20 µg
Lane 2: HepG2 lysate at 20 µg
Lane 3: HeLa lysate at 20 µg
Lane 4: SH-SY5Y lysate at 20 µg
All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling NXN/NRX with ab191429 at 1/250, Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 and DAPI staining (blue). The two negative controls: 1. Primary ab concentration (anti-NXN/NRX) is 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/400 dilution; 2. Primary ab concentration (anti-NXN/NRX) is 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/400 dilution.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue labeling NXN/NRX with ab191429 at 1/100 with prediluted HRP Polymer for Rabbit IgG as secondary antibody. Negative control: Using PBS instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder tissue labeling NXN/NRX with ab191429 at 1/100 with prediluted HRP Polymer for Rabbit IgG as secondary antibody. Negative control: Using PBS instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
All lanes: Western blot - Anti-NXN/NRX antibody [EPR15177] (ab191429) at 1/10000 dilution
Lane 1: Mouse testis lysate at 10 µg
Lane 2: Rat testis lysate at 10 µg
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
Intracellular Flow Cytometry analysis of NIH/3T3 (mouse embryo) labelling NXN/NRXwith purified ab191429 at 1/1000 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Alexa Fluor® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
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