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Rabbit Recombinant Monoclonal OAS2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

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Images

Sandwich ELISA - Anti-OAS2 antibody [EPR26734-22] - BSA and Azide free (Detector) (AB322970), expandable thumbnail
  • Sandwich ELISA - Anti-OAS2 antibody [EPR26734-22] - BSA and Azide free (Detector) (AB322970), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Human
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Target data

Function

Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response (PubMed:10464285, PubMed:9880569). Activated by detection of double stranded RNA (dsRNA): polymerizes higher oligomers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNASEL) leading to its dimerization and subsequent activation (PubMed:10464285, PubMed:11682059, PubMed:9880569). Activation of RNASEL leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication (PubMed:10464285, PubMed:9880569). Can mediate the antiviral effect via the classical RNASEL-dependent pathway or an alternative antiviral pathway independent of RNASEL (PubMed:21142819). In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation (PubMed:21142819). May act as a negative regulator of lactation, stopping lactation in virally infected mammary gland lobules, thereby preventing transmission of viruses to neonates (By similarity). Non-infected lobules would not be affected, allowing efficient pup feeding during infection (By similarity).

Alternative names

2'-5'-oligoadenylate synthase 2, (2-5')oligo(A) synthase 2, 2-5A synthase 2, p69 OAS / p71 OAS, p69OAS / p71OAS, OAS2

Rabbit Recombinant Monoclonal OAS2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR26734-22
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab322970 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human OAS2. The recommended pair for sandwich ELISA is:

Capture: Anti-OAS2 antibody [EPR26734-27] - BSA and Azide free (Capture) ab322969, Human OAS2 Capture Antibody (unconjugated)

Detector: ab322970, Human OAS2 Detector Antibody (unconjugated)

The reference range value is 0.31-20 ng/mL.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Sandwich ELISA - Anti-OAS2 antibody [EPR26734-22] - BSA and Azide free (Detector) (ab322970), expandable thumbnail

    Sandwich ELISA - Anti-OAS2 antibody [EPR26734-22] - BSA and Azide free (Detector) (ab322970)

    Sandwich ELISA of Human OAS2 Antibody Pair - BSA and Azide free ab322968 with the capture antibody Anti-OAS2 antibody [EPR26734-27] - BSA and Azide free (Capture) ab322969 dilution at 2 ug/mL and detector antibody ab322970 dilution at 0.5 ug/mL.

  • Sandwich ELISA - Anti-OAS2 antibody [EPR26734-22] - BSA and Azide free (Detector) (ab322970), expandable thumbnail

    Sandwich ELISA - Anti-OAS2 antibody [EPR26734-22] - BSA and Azide free (Detector) (ab322970)

    Interpolated concentration of native OAS2 was measured at different sample concentrations. Undiluted samples are as follows: stimulated HeLa cell extract (treated with 10 ng/mL IFN-alpha for 16 hours) (62.5 µg/mL) and mock unstimulated HeLa cell extract (62.5 µg/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in antibody diluent CP2 (Antibody Diluent CP2 ab319981). Interpolated concentrations of OAS2 in mock unstimulated HeLa cell extract were below the detectable range of the assay. Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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