Rabbit Polyclonal OAS3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Human OAS3 aa 50-400.
View Alternative Names
P/OKcl.4, OAS3, 2'-5'-oligoadenylate synthase 3, (2-5')oligo(A) synthase 3, 2-5A synthase 3, p100 OAS, p100OAS
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OAS3 antibody (AB154270)
Immunohistochemical analysis of paraffin-embedded Human D54MG xenograft tissue, labeling OAS3 with ab154270 at 1/500 dilution.
- WB
Unknown
Western blot - Anti-OAS3 antibody (AB154270)
7.5% SDS PAGE
All lanes:
Western blot - Anti-OAS3 antibody (ab154270) at 1/3000 dilution
All lanes:
A431 whole cell lysate at 30 µg
Predicted band size: 121 kDa
false
- WB
CiteAb
Western blot - Anti-OAS3 antibody (AB154270)
Western Blotting using Anti-OAS3 antibody, ab154270. Publication image from Odon, V. et al., 2019, Nucleic Acids Res, 31276592. Legend direct from paper.
Induction of ZAP, RNAseL and OAS3 on E7 infection. (A) Induction of ZAP (L and S isoforms), RNAseL and OAS3 on infection with E7 over 10 h post-infection with E7 and compositionally altered mutants. (B) Effects of IRF3 pathway inhibition by NPro and of ZAP k/o on RNAseL and OAS3 expression in cells infected with E7 WT.
false
- WB
CiteAb
Western blot - Anti-OAS3 antibody (AB154270)
Western Blotting using Anti-OAS3 antibody, ab154270. Publication image from Odon, V. et al., 2019, Nucleic Acids Res, 31276592. Legend direct from paper.
Expression of ZAP, RNAseL and OAS3 in cell lines; association of ZAP expression with attenuation. Detection of ZAP-L and ZAP-S (A) and RNAseL, and OAS3 (B) by western blot analysis of lysates of different cell lines before and after stimulation with exogenous IFN-β. (C) Association of ZAP-L expression with the attenuation index of mutants of E7 with high CpG or UpA 3′UTR sequences. The black and grey dotted lines show lines of best fit and associated R values. Significance levels were based on one-tailed probabilities using the Spearman non-parametric test.
false
Reactivity data
Properties and storage information
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Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
OAS3 contributes to the host's defense by activating RNase L a ribonuclease that degrades viral and cellular RNA to limit viral replication. OAS3 functions as part of the larger oligoadenylate synthetase family complex which enhances its ability to mediate an efficient antiviral response. The protein requires double-stranded RNA often produced during viral infections to achieve activation. This activation links the detection of viral components directly to antiviral actions effectively curtailing the spread of the virus within the host.
Pathways
OAS3 participates in the interferon alpha/beta signaling pathway and the 2'-5'-oligoadenylate synthetase-RNase L pathway. These pathways are essential for mediating antiviral responses in innate immunity. In these pathways OAS3 interacts with related proteins such as RNase L and other members of the OAS family like OAS1 and OAS2 which collectively enhance the immune response against viral threats.
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Target data
Publications (10)
Recent publications for all applications. Explore the full list and refine your search
Frontiers in immunology 15:1343805 PubMed39403387
2024
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The Journal of biological chemistry 300:107783 PubMed39303913
2024
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Nucleic acids research 52:11128-11147 PubMed38917323
2024
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Molecular oncology 17:2584-2602 PubMed37746742
2023
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Nature 614:358-366 PubMed36725932
2023
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Journal of immunology research 2022:8873536 PubMed35928633
2022
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Molecular therapy oncolytics 17:496-507 PubMed32529027
2020
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Nucleic acids research 47:8061-8083 PubMed31276592
2019
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Biochemical and biophysical research communication 511:422-426 PubMed30799083
2019
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Nucleic acids research 47:432-449 PubMed30395302
2018
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Product promise
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