Rabbit Polyclonal OAS3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human OAS3 aa 50-400.
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Publications
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- Nature 614:358-3662023mRNA ageing shapes the Cap2 methylome in mammalian mRNA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVladimir Despic,Samie R JaffreyPubMed 36725932
- Journal of immunology research 2022:88735362022The Pharmacologically Active Alkaloid Cryptolepine Activates a Type 1 Interferon Response That Is Independent of MAVS and STING Pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeth A Domfeh,Patrick W Narkwa,Osbourne Quaye,Kwadwo A Kusi,Bright S Addy,Sian Lant,Rebecca P Sumner,Carlos Maluquer de Motes,Gordon A Awandare,Charles Ansah,Mohamed MutocheluhPubMed 35928633
- Molecular therapy oncolytics 17:496-5072020and Are Novel Mediators of Resistance to Oncolytic Vesicular Stomatitis Virus in Prostate Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRobert S Bayne et. al.PubMed 32529027
- Nucleic acids research 47:8061-80832019The role of ZAP and OAS3/RNAseL pathways in the attenuation of an RNA virus with elevated frequencies of CpG and UpA dinucleotides.Applications:Unspecified applicationReactive species:Unspecified reactive speciesValerie Odon,Jelke J Fros,Niluka Goonawardane,Isabelle Dietrich,Ahmad Ibrahim,Kinda Alshaikhahmed,Dung Nguyen,Peter SimmondsPubMed 31276592
- Biochemical and biophysical research communication 511:422-4262019A novel method for stabilizing microRNA mimics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTakuto Nogimori et. al.PubMed 30799083
- Nucleic acids research 47:432-4492018Dom34 mediates targeting of exogenous RNA in the antiviral OAS/RNase L pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTakuto Nogimori,Kyutatsu Nishiura,Sho Kawashima,Takahiro Nagai,Yuka Oishi,Nao Hosoda,Hiroaki Imataka,Yoshiaki Kitamura,Yukio Kitade,Shin-Ichi HoshinoPubMed 30395302
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human OAS3 aa 50-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y6K5
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Target data
Function
Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes preferentially dimers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L. Displays antiviral activity against Chikungunya virus (CHIKV), Dengue virus, Sindbis virus (SINV) and Semliki forest virus (SFV).
Alternative names
P/OKcl.4, OAS3, 2'-5'-oligoadenylate synthase 3, (2-5')oligo(A) synthase 3, 2-5A synthase 3, p100 OAS, p100OAS
Recommended products
Rabbit Polyclonal OAS3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human OAS3 aa 50-400.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human OAS3 aa 50-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y6K5
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The 2'-5'-oligoadenylate synthetase 3 (OAS3) protein also known as OAS03 OAS-treate or simply OAS003 plays an important role in the innate immune response to viral infections. OAS3 is a member of the 2'-5'-oligoadenylate synthetase family and possesses a mass of approximately 125 kDa. It is ubiquitously expressed in various tissues but shows higher expression levels in liver lung and spleen. This protein is involved in the synthesis of 2'-5'-linked oligoadenylates from ATP an activity which is important for antiviral defense mechanisms in the cell.
- Biological function summary
OAS3 contributes to the host's defense by activating RNase L a ribonuclease that degrades viral and cellular RNA to limit viral replication. OAS3 functions as part of the larger oligoadenylate synthetase family complex which enhances its ability to mediate an efficient antiviral response. The protein requires double-stranded RNA often produced during viral infections to achieve activation. This activation links the detection of viral components directly to antiviral actions effectively curtailing the spread of the virus within the host.
- Pathways
OAS3 participates in the interferon alpha/beta signaling pathway and the 2'-5'-oligoadenylate synthetase-RNase L pathway. These pathways are essential for mediating antiviral responses in innate immunity. In these pathways OAS3 interacts with related proteins such as RNase L and other members of the OAS family like OAS1 and OAS2 which collectively enhance the immune response against viral threats.
- Associated diseases and disorders
Alterations in OAS3 activity relate to diseases such as hepatitis and systemic lupus erythematosus (SLE). Abnormal OAS3 expression or function can affect host viral defense mechanisms contributing to chronic infection conditions like hepatitis. In SLE OAS3 alongside proteins like OAS1 and RNase L becomes relevant due to its role in inappropriately activating immune responses potentially leading to tissue damage. These insights into OAS3's role highlight its significance in both protective immunity and its potential involvement in autoimmunity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-OAS3 antibody (ab154270)
7.5% SDS PAGE
All lanes: Western blot - Anti-OAS3 antibody (ab154270) at 1/3000 dilution
All lanes: A431 whole cell lysate at 30 µg
Predicted band size: 121 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OAS3 antibody (ab154270)
Immunohistochemical analysis of paraffin-embedded Human D54MG xenograft tissue, labeling OAS3 with ab154270 at 1/500 dilution.
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