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AB154270

Anti-OAS3 antibody

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(10 Publications)

Rabbit Polyclonal OAS3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Human OAS3 aa 50-400.

View Alternative Names

P/OKcl.4, OAS3, 2'-5'-oligoadenylate synthase 3, (2-5')oligo(A) synthase 3, 2-5A synthase 3, p100 OAS, p100OAS

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OAS3 antibody (AB154270)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OAS3 antibody (AB154270)

Immunohistochemical analysis of paraffin-embedded Human D54MG xenograft tissue, labeling OAS3 with ab154270 at 1/500 dilution.

Western blot - Anti-OAS3 antibody (AB154270)
  • WB

Unknown

Western blot - Anti-OAS3 antibody (AB154270)

7.5% SDS PAGE

All lanes:

Western blot - Anti-OAS3 antibody (ab154270) at 1/3000 dilution

All lanes:

A431 whole cell lysate at 30 µg

Predicted band size: 121 kDa

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Western blot - Anti-OAS3 antibody (AB154270)
  • WB

CiteAb

Western blot - Anti-OAS3 antibody (AB154270)

Western Blotting using Anti-OAS3 antibody, ab154270. Publication image from Odon, V. et al., 2019, Nucleic Acids Res, 31276592. Legend direct from paper.

Induction of ZAP, RNAseL and OAS3 on E7 infection. (A) Induction of ZAP (L and S isoforms), RNAseL and OAS3 on infection with E7 over 10 h post-infection with E7 and compositionally altered mutants. (B) Effects of IRF3 pathway inhibition by NPro and of ZAP k/o on RNAseL and OAS3 expression in cells infected with E7 WT.

false

Western blot - Anti-OAS3 antibody (AB154270)
  • WB

CiteAb

Western blot - Anti-OAS3 antibody (AB154270)

Western Blotting using Anti-OAS3 antibody, ab154270. Publication image from Odon, V. et al., 2019, Nucleic Acids Res, 31276592. Legend direct from paper.

Expression of ZAP, RNAseL and OAS3 in cell lines; association of ZAP expression with attenuation. Detection of ZAP-L and ZAP-S (A) and RNAseL, and OAS3 (B) by western blot analysis of lysates of different cell lines before and after stimulation with exogenous IFN-β. (C) Association of ZAP-L expression with the attenuation index of mutants of E7 with high CpG or UpA 3′UTR sequences. The black and grey dotted lines show lines of best fit and associated R values. Significance levels were based on one-tailed probabilities using the Spearman non-parametric test.

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human OAS3 aa 50-400. The exact immunogen used to generate this antibody is proprietary information.

Q9Y6K5

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 2'-5'-oligoadenylate synthetase 3 (OAS3) protein also known as OAS03 OAS-treate or simply OAS003 plays an important role in the innate immune response to viral infections. OAS3 is a member of the 2'-5'-oligoadenylate synthetase family and possesses a mass of approximately 125 kDa. It is ubiquitously expressed in various tissues but shows higher expression levels in liver lung and spleen. This protein is involved in the synthesis of 2'-5'-linked oligoadenylates from ATP an activity which is important for antiviral defense mechanisms in the cell.
Biological function summary

OAS3 contributes to the host's defense by activating RNase L a ribonuclease that degrades viral and cellular RNA to limit viral replication. OAS3 functions as part of the larger oligoadenylate synthetase family complex which enhances its ability to mediate an efficient antiviral response. The protein requires double-stranded RNA often produced during viral infections to achieve activation. This activation links the detection of viral components directly to antiviral actions effectively curtailing the spread of the virus within the host.

Pathways

OAS3 participates in the interferon alpha/beta signaling pathway and the 2'-5'-oligoadenylate synthetase-RNase L pathway. These pathways are essential for mediating antiviral responses in innate immunity. In these pathways OAS3 interacts with related proteins such as RNase L and other members of the OAS family like OAS1 and OAS2 which collectively enhance the immune response against viral threats.

Alterations in OAS3 activity relate to diseases such as hepatitis and systemic lupus erythematosus (SLE). Abnormal OAS3 expression or function can affect host viral defense mechanisms contributing to chronic infection conditions like hepatitis. In SLE OAS3 alongside proteins like OAS1 and RNase L becomes relevant due to its role in inappropriately activating immune responses potentially leading to tissue damage. These insights into OAS3's role highlight its significance in both protective immunity and its potential involvement in autoimmunity.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes preferentially dimers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L. Displays antiviral activity against Chikungunya virus (CHIKV), Dengue virus, Sindbis virus (SINV) and Semliki forest virus (SFV).
See full target information OAS3

Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 15:1343805 PubMed39403387

2024

Unlocking the secrets of NPSLE: the role of dendritic cell-secreted CCL2 in blood-brain barrier disruption.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Wang,Guimin Zheng,Peiwen Wang,Xiuchuan Jia

The Journal of biological chemistry 300:107783 PubMed39303913

2024

Downregulation of the mA reader YTHDC2 upregulates exosome content in lung adenocarcinoma via inhibiting IFIT and OAS family members.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixin Yin,Lifang Ma,Xiaoting Tian,Qi Sun,Congcong Zhang,Yikun Wang,Yayou Miao,Xiangfei Xue,Yongjie Wang,Jiayi Wang,Xiao Zhang,Xumin Hou

Nucleic acids research 52:11128-11147 PubMed38917323

2024

Zika virus non-coding RNAs antagonize antiviral responses by PKR-mediated translational arrest.

Applications

Unspecified application

Species

Unspecified reactive species

Horacio M Pallarés,María Mora González López Ledesma,Santiago Oviedo-Rouco,Luciana A Castellano,Guadalupe S Costa Navarro,Ana J Fernández-Alvarez,María Josefina D'Andreiz,Victor Daniel Aldas-Bulos,Diego E Alvarez,Ariel A Bazzini,Andrea V Gamarnik

Molecular oncology 17:2584-2602 PubMed37746742

2023

Downregulation of RCN1 promotes pyroptosis in acute myeloid leukemia cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sisi Deng,Yuming Pan,Na An,Fengyi Chen,Huan Chen,Heng Wang,Xiaojing Xu,Rui Liu,Linlin Yang,Xiaomei Wang,Xin Du,Qiaoxia Zhang

Nature 614:358-366 PubMed36725932

2023

mRNA ageing shapes the Cap2 methylome in mammalian mRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Vladimir Despic,Samie R Jaffrey

Journal of immunology research 2022:8873536 PubMed35928633

2022

The Pharmacologically Active Alkaloid Cryptolepine Activates a Type 1 Interferon Response That Is Independent of MAVS and STING Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Seth A Domfeh,Patrick W Narkwa,Osbourne Quaye,Kwadwo A Kusi,Bright S Addy,Sian Lant,Rebecca P Sumner,Carlos Maluquer de Motes,Gordon A Awandare,Charles Ansah,Mohamed Mutocheluh

Molecular therapy oncolytics 17:496-507 PubMed32529027

2020

and Are Novel Mediators of Resistance to Oncolytic Vesicular Stomatitis Virus in Prostate Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Robert S Bayne,Shelby Puckett,Lindsey Ulkus Rodrigues,Scott D Cramer,Jingyun Lee,Cristina M Furdui,Jeff W Chou,Lance D Miller,David A Ornelles,Douglas S Lyles

Nucleic acids research 47:8061-8083 PubMed31276592

2019

The role of ZAP and OAS3/RNAseL pathways in the attenuation of an RNA virus with elevated frequencies of CpG and UpA dinucleotides.

Applications

Unspecified application

Species

Unspecified reactive species

Valerie Odon,Jelke J Fros,Niluka Goonawardane,Isabelle Dietrich,Ahmad Ibrahim,Kinda Alshaikhahmed,Dung Nguyen,Peter Simmonds

Biochemical and biophysical research communication 511:422-426 PubMed30799083

2019

A novel method for stabilizing microRNA mimics.

Applications

Unspecified application

Species

Unspecified reactive species

Takuto Nogimori,Kazuya Furutachi,Koichi Ogami,Nao Hosoda,Shin-Ichi Hoshino

Nucleic acids research 47:432-449 PubMed30395302

2018

Dom34 mediates targeting of exogenous RNA in the antiviral OAS/RNase L pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Takuto Nogimori,Kyutatsu Nishiura,Sho Kawashima,Takahiro Nagai,Yuka Oishi,Nao Hosoda,Hiroaki Imataka,Yoshiaki Kitamura,Yukio Kitade,Shin-Ichi Hoshino
View all publications

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