AB229136

Anti-OASL antibody

Name: Anti-OASL antibody (ab229136) | Abcam
Brand: Abcam Limited
SKU: AB229136

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(7 Publications)

Rabbit Polyclonal OASL antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human OASL.

View Alternative Names

TRIP14, OASL, 2'-5'-oligoadenylate synthase-like protein, 2'-5'-OAS-related protein, 59 kDa 2'-5'-oligoadenylate synthase-like protein, Thyroid receptor-interacting protein 14, p59 OASL, 2'-5'-OAS-RP, TR-interacting protein 14, TRIP-14, p59OASL

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-OASL antibody (AB229136)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-OASL antibody (AB229136)

Paraformaldehyde-fixed Hela (human epithelial cell line from cervix adenocarcinoma) cells stained for OASL (green) using ab229136 at 1/500 dilution in ICC/IF.

Nuclear counterstain : Hoechst 33342 (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OASL antibody (AB229136)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OASL antibody (AB229136)

Paraffin-embedded human breast cancer tissue stained for OASL using ab229136 at 1/250 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-OASL antibody (AB229136)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-OASL antibody (ab229136) at 1/2000 dilution

All lanes:

HCT116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 59 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human OASL. The exact immunogen used to generate this antibody is proprietary information.

Q15646

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OASL also known as 2'-5'-oligoadenylate synthetase-like protein functions mechanically by enhancing antiviral defense mechanisms. This protein weighs approximately 58 kDa and is expressed in various cell types including immune cells such as monocytes and lymphocytes. OASL belongs to the family of interferon-stimulated genes and shares structural similarities with other 2'-5'-oligoadenylate synthetases.

Biological function summary

OASL plays a significant role in the immune system's response to viral infections. It does not form a complex but aids in the synthesis of 2'-5'-linked oligoadenylates which activate RNase L leading to the degradation of viral RNA. By doing so OASL helps to restrict viral replication and propagation within the host organism strengthening the antiviral response.

Pathways

OASL is actively involved in the interferon signaling pathway and the innate immune response pathway. It works closely with other proteins such as RIG-I-like receptors which are essential for detecting viral RNA and triggering downstream signaling processes. These interactions highlight OASL's integral function in coordinating antiviral defense strategies within these pathways.

OASL has connections to viral infections and autoimmune disorders. During infections by viruses like hepatitis C OASL contributes to the host's defense by limiting viral replication. Additionally its involvement in autoimmune conditions such as systemic lupus erythematosus demonstrates its role in immune system regulation. In these contexts OASL associates with proteins like MAVS which facilitate innate immune signaling and modulate disease mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Does not have 2'-5'-OAS activity, but can bind double-stranded RNA. Displays antiviral activity against encephalomyocarditis virus (EMCV) and hepatitis C virus (HCV) via an alternative antiviral pathway independent of RNase L.

See full target information OASL

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 133:e70056 PubMed40988101

2025

Combination of Isoliquiritigenin and Vancomycin Alleviates Staphylococcus aureus-Induced Bone Infection in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Mingbo Wang,Huicheng Lv,Long Han,Haisheng Jia,Lifeng Zhang,Lei Wang,Aimin He,Yu Du

Frontiers in immunology 15:1402951 PubMed39286258

2024

Multi-omics landscape of Interferon-stimulated gene OASL reveals a potential biomarker in pan-cancer: from prognosis to tumor microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Liu,Runyu Yang,Mengyao Zhang,Bingyu Yang,Yue Du,Hui Feng,Wenjuan Wang,Busheng Xue,Fan Niu,Pengcheng He

Molecular carcinogenesis 63:938-950 PubMed38353288

2024

Peptidase inhibitor 16 promotes proliferation of pancreatic ductal adenocarcinoma cells through OASL signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Qun Chen,Lu-Yang Jiang,Cheng Cao,Feng-Yuan Liu,Dan-Rui Li,Peng-Fei Wu,Kui-Rong Jiang

Viruses 15: PubMed37631988

2023

Host DNA Demethylation Induced by DNMT1 Inhibition Up-Regulates Antiviral OASL Protein during Influenza a Virus Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiyan Zhao,Jing Li,Ye Feng,Xiaoping Kang,Yuchang Li,Yuehong Chen,Wei Li,Wenguang Yang,Lu Zhao,Shenghai Huang,Sen Zhang,Tao Jiang

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e22824 PubMed36809539

2023

OASL knockdown inhibits the progression of stomach adenocarcinoma by regulating the mTORC1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Weizhu Zhao,Haiying Yang,Luguang Liu,Xianlin Qu,Jishuang Ding,Hang Yu,Botao Xu,Siwei Zhao,Guangmin Xi,Ligang Xing,Jie Chai

Arthritis research & therapy 24:50 PubMed35183246

2022

Overexpression of OASL upregulates TET1 to induce aberrant activation of CD4 T cells in systemic sclerosis via IRF1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuotong Zeng,Yaoyao Wang,Yangfan Xiao,Jie Zheng,Ruizhen Liu,Xinglan He,Jiangfan Yu,Bingsi Tang,Xiangning Qiu,Rui Tang,Yaqian Shi,Rong Xiao

Cell death & disease 12:585 PubMed34099637

2021

TRPM2 promotes pancreatic cancer by PKC/MAPK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Lin,Xunxia Bao,Hui Wang,Sibo Zhu,Zhongyan Liu,Quanning Chen,Kaixing Ai,Baomin Shi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-OASL antibody

Immunocytochemistry/ Immunofluorescence - Anti-OASL antibody (AB229136)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OASL antibody (AB229136)

Western blot - Anti-OASL antibody (AB229136)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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