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AB317217

Anti-OASL antibody [HL2399]

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Rabbit Monoclonal OASL antibody. Suitable for WB and reacts with Transfected cell lysate, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human OASL.

View Alternative Names

TRIP14, OASL, 2'-5'-oligoadenylate synthase-like protein, 2'-5'-OAS-related protein, 59 kDa 2'-5'-oligoadenylate synthase-like protein, Thyroid receptor-interacting protein 14, p59 OASL, 2'-5'-OAS-RP, TR-interacting protein 14, TRIP-14, p59OASL

2 Images
Western blot - Anti-OASL antibody [HL2399] (AB317217)
  • WB

Supplier Data

Western blot - Anti-OASL antibody [HL2399] (AB317217)

Untreated (–) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE and the membrane was blotted with ab317217 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-OASL antibody [HL2399] (ab317217) at 1/1000 dilution

Lane 1:

Untreated (–) HeLa whole cell extracts at 30 µg

Lane 2:

100 ug/mL Poly (I:C) treated (+) HeLa whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-OASL antibody [HL2399] (AB317217)
  • WB

Supplier Data

Western blot - Anti-OASL antibody [HL2399] (AB317217)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE and the membrane was blotted with ab317217 diluted at 1 : 10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-OASL antibody [HL2399] (ab317217) at 1/10000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tagged OASL transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2399

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human OASL.

Q15646

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OASL also known as 2'-5'-oligoadenylate synthetase-like protein functions mechanically by enhancing antiviral defense mechanisms. This protein weighs approximately 58 kDa and is expressed in various cell types including immune cells such as monocytes and lymphocytes. OASL belongs to the family of interferon-stimulated genes and shares structural similarities with other 2'-5'-oligoadenylate synthetases.
Biological function summary

OASL plays a significant role in the immune system's response to viral infections. It does not form a complex but aids in the synthesis of 2'-5'-linked oligoadenylates which activate RNase L leading to the degradation of viral RNA. By doing so OASL helps to restrict viral replication and propagation within the host organism strengthening the antiviral response.

Pathways

OASL is actively involved in the interferon signaling pathway and the innate immune response pathway. It works closely with other proteins such as RIG-I-like receptors which are essential for detecting viral RNA and triggering downstream signaling processes. These interactions highlight OASL's integral function in coordinating antiviral defense strategies within these pathways.

OASL has connections to viral infections and autoimmune disorders. During infections by viruses like hepatitis C OASL contributes to the host's defense by limiting viral replication. Additionally its involvement in autoimmune conditions such as systemic lupus erythematosus demonstrates its role in immune system regulation. In these contexts OASL associates with proteins like MAVS which facilitate innate immune signaling and modulate disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Does not have 2'-5'-OAS activity, but can bind double-stranded RNA. Displays antiviral activity against encephalomyocarditis virus (EMCV) and hepatitis C virus (HCV) via an alternative antiviral pathway independent of RNase L.
See full target information OASL
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Product promise

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