AB308226

Anti-OAT antibody [HL2087] - BSA and Azide free

Recombinant
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Rabbit Recombinant Monoclonal ornithine aminotransferase antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human OAT.

View Alternative Names

Ornithine delta-aminotransferase, Ornithine--oxo-acid aminotransferase, OAT

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-OAT antibody [HL2087] - BSA and Azide free (AB308226)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-OAT antibody [HL2087] - BSA and Azide free (AB308226)

Immunofluorescent analysis of A431 cells labelling OAT with ab308226 at 1/500 dilution fixed in ice-cold MeOH for 5 min.

Blue : Fluoroshield with DAPI.

Scale bar= 10µm.

Supplier Data

Western blot - Anti-OAT antibody [HL2087] - BSA and Azide free (AB308226)

Samples were separated by 10% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-OAT antibody [HL2087] - BSA and Azide free (ab308226) at 1/1000 dilution

Lane 1:

K562 whole cell extracts at 30 µg

Lane 2:

THP-1 whole cell extracts at 30 µg

Lane 3:

HL-60 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 48 kDa

false

Supplier Data

Western blot - Anti-OAT antibody [HL2087] - BSA and Azide free (AB308226)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-OAT antibody [HL2087] - BSA and Azide free (ab308226) at 1/5000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

Transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 48 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2087

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human OAT. The exact immunogen used to generate this antibody is proprietary information.

P04181

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: 100% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ornithine aminotransferase also known as ornithine transaminase is an enzyme that facilitates the conversion of ornithine and α-ketoglutarate to glutamate semialdehyde and glutamate. This enzyme weighs around 49 kDa. It shows expression mainly in the liver kidney small intestine and retina. The enzyme works inside cellular mitochondria and assists by catalyzing amino group transfer reactions. It is one member of the transaminase family.

Biological function summary

This enzyme plays a role in the urea cycle and amino acid metabolism. It helps in the conversion of excess nitrogen into less toxic forms that the body can excrete. As a part of an important metabolic network it influences the synthesis and degradation of important biological molecules. It operates independently and does not form known protein complexes for its function.

Pathways

Ornithine aminotransferase is important in the ornithine and glutamate metabolism pathways. Within the urea cycle it cooperates with enzymes such as arginase and carbamoyl phosphate synthetase to regulate nitrogen balance in cells. Additionally it engages in the proline-glutamate pathway which involves proteins like proline dehydrogenase analzying the reversible conversion between proline and glutamate.

Ornithine aminotransferase associates with gyrate atrophy a rare genetic condition that affects the eye and leads to vision loss. Deficiencies in this enzyme can result in abnormal accumulations of ornithine in the blood. Some connections also exist with hyperammonemia where impaired nitrogen excretion causes elevated ammonia levels. In these conditions the enzyme's defective activity links to mutations or irregular interactions with proteins like glutamate dehydrogenase affecting metabolic balance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the reversible interconversion of L-ornithine and 2-oxoglutarate to L-glutamate semialdehyde and L-glutamate.

See full target information OAT

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-OAT antibody [HL2087] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-OAT antibody [HL2087] - BSA and Azide free (AB308226)

Western blot - Anti-OAT antibody [HL2087] - BSA and Azide free (AB308226)

Western blot - Anti-OAT antibody [HL2087] - BSA and Azide free (AB308226)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise