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AB317132

Anti-OAT antibody [HL2088]

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Rabbit Monoclonal ornithine aminotransferase antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Mouse, Rat, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human OAT.

View Alternative Names

Ornithine delta-aminotransferase, Ornithine--oxo-acid aminotransferase, OAT

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-OAT antibody [HL2088] (AB317132)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-OAT antibody [HL2088] (AB317132)

ab317132 detects OAT protein at mitochondria and cytoplasm by immunofluorescent analysis.
Sample : A431 cells were fixed in ice-cold MeOH for 5 min.
Green : OAT stained by ab317132 diluted at 1 : 500.
Blue : Fluoroshield with DAPI.
Scale bar = 10um.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OAT antibody [HL2088] (AB317132)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OAT antibody [HL2088] (AB317132)

ab317132 detects OAT protein at cytoplasm by immunohistochemical analysis.
Sample : Paraffin-embedded mouse lung.
OAT stained by ab317132 diluted at 1 : 100.
Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OAT antibody [HL2088] (AB317132)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OAT antibody [HL2088] (AB317132)

ab317132 detects OAT protein at cytoplasm by immunohistochemical analysis.
Sample : Paraffin-embedded rat duodenum.
OAT stained by ab317132 diluted at 1 : 100.
Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Western blot - Anti-OAT antibody [HL2088] (AB317132)
  • WB

Supplier Data

Western blot - Anti-OAT antibody [HL2088] (AB317132)

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE and the membrane was blotted with ab317132 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-OAT antibody [HL2088] (ab317132) at 1/1000 dilution

Lane 1:

K562 whole cell extracts at 30 µg

Lane 2:

THP-1 whole cell extracts at 30 µg

Lane 3:

HL-60 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2088

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human OAT.

P04181

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ornithine aminotransferase also known as ornithine transaminase is an enzyme that facilitates the conversion of ornithine and α-ketoglutarate to glutamate semialdehyde and glutamate. This enzyme weighs around 49 kDa. It shows expression mainly in the liver kidney small intestine and retina. The enzyme works inside cellular mitochondria and assists by catalyzing amino group transfer reactions. It is one member of the transaminase family.
Biological function summary

This enzyme plays a role in the urea cycle and amino acid metabolism. It helps in the conversion of excess nitrogen into less toxic forms that the body can excrete. As a part of an important metabolic network it influences the synthesis and degradation of important biological molecules. It operates independently and does not form known protein complexes for its function.

Pathways

Ornithine aminotransferase is important in the ornithine and glutamate metabolism pathways. Within the urea cycle it cooperates with enzymes such as arginase and carbamoyl phosphate synthetase to regulate nitrogen balance in cells. Additionally it engages in the proline-glutamate pathway which involves proteins like proline dehydrogenase analzying the reversible conversion between proline and glutamate.

Ornithine aminotransferase associates with gyrate atrophy a rare genetic condition that affects the eye and leads to vision loss. Deficiencies in this enzyme can result in abnormal accumulations of ornithine in the blood. Some connections also exist with hyperammonemia where impaired nitrogen excretion causes elevated ammonia levels. In these conditions the enzyme's defective activity links to mutations or irregular interactions with proteins like glutamate dehydrogenase affecting metabolic balance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the reversible interconversion of L-ornithine and 2-oxoglutarate to L-glutamate semialdehyde and L-glutamate.
See full target information OAT
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Product promise

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