Anti-OATP1B1 antibody [EPR27070-49]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OATP1B1 antibody [EPR27070-49] (AB312838)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular tissue labeling OATP1B1 with ab312838 at 1/2000 (0.255 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human hepatocellular carcinoma. The section was incubated with ab312838 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OATP1B1 antibody [EPR27070-49] (AB312838)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling OATP1B1 with ab312838 at 1/2000 (0.255 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human liver. The section was incubated with ab312838 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• WB

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Western blot - Anti-OATP1B1 antibody [EPR27070-49] (AB312838)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : Human kidney, Human skeletal muscle (PMID : 10644574). The molecular weight observed is consistent with what has been described in the literature (PMID : 30970235, 16697742). Lane 1 lysate was prepared via the 1% SDS HOT lysis method, lanes 2-4 were prepared via RIPA lysis. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 10 seconds

All lanes:

Western blot - Anti-OATP1B1 antibody [EPR27070-49] (ab312838) at 1/1000 dilution

Lane 1:

Human liver tissue lysate at 20 µg

Lane 2:

Human liver cancer tissue lysate at 20 µg

Lane 3:

Human kidney tissue lysate at 20 µg

Lane 4:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 72 kDa,95 kDa

false

Exposure time: 10s

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OATP1B1 antibody [EPR27070-49] (AB312838)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling OATP1B1 with ab312838 at 1/2000 (0.255 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on human kidney. The section was incubated with ab312838 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• Dot

Supplier Data

Dot Blot - Anti-OATP1B1 antibody [EPR27070-49] (AB312838)

Dot blot analysis of OATP1B1 using ab312838 at 1 : 1000 (0.51 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution. Lane 1 : 293T cells transfected with a human OATPB3 expression vector containing a His-tag whole cell lysate 1 μg Lane 2 : 293T cells transfected with a human OATPB7 expression vector containing a His-tag whole cell lysate 100 ng Lane 3 : 293T cells transfected with a human OATPB1 expression vector containing a His-tag whole cell lysate 50 ng Exposure time : 15 sceonds Blocking and diluting buffer and concentration : 5% NFDM/TBST