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AB15442

Anti-OATP1B1 + OATP1B3 antibody [MDQ]

2

(2 Reviews)

|

(3 Publications)

Mouse Monoclonal OATP1B1 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human SLCO1B1 aa 1-50.

View Alternative Names

LST1, OATP1B1, OATP2, OATPC, SLC21A6, SLCO1B1, Solute carrier organic anion transporter family member 1B1, Liver-specific organic anion transporter 1, OATP-C, Organic anion transporter SLC21A6, Sodium-independent organic anion-transporting polypeptide 2, Solute carrier family 21 member 6, LST-1, OATP-2, LST2, OATP1B3, OATP8, SLC21A8, SLCO1B3, Solute carrier organic anion transporter family member 1B3, Liver-specific organic anion transporter 2, Organic anion transporter 8, Organic anion-transporting polypeptide 8, Solute carrier family 21 member 8, LST-2, OATP-8

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-OATP1B1 + OATP1B3 antibody [MDQ] (AB15442)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-OATP1B1 + OATP1B3 antibody [MDQ] (AB15442)

Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labeling OATP1B1 + OATP1B3 (green) with ab15542 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MDQ

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Synthetic Peptide within Human SLCO1B1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9Y6L6

Specificity

ab15442 will also cross react with OATP8.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/20", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/20", "ICCIF-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/20", "ICCIF-species-notes": "<p></p>" }, "Dog": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/20", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
Preservative: 0.09% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OATP1B1 also known as SLCO1B1 and OATP1B3 also known as SLCO1B3 are members of the organic anion transporting polypeptide family. These proteins serve as key transporters involved in the uptake of a wide range of endogenous and exogenous compounds including drugs into hepatocytes. OATP1B1 has a molecular mass of approximately 85 kDa and is predominantly expressed in the human liver. OATP1B3 shares similar characteristics but possesses distinct substrate specificity and expression patterns being localized similarly in the liver but also found in other tissues such as the pancreas. These transporters facilitate the movement of substances across cellular membranes influencing drug disposition and metabolism.
Biological function summary

OATP1B1 and OATP1B3 participate in the sodium-independent transport of bile acids bilirubin and various drugs. They do not typically form part of larger protein complexes but interact closely with other hepatic transporters to regulate the enterohepatic circulation of bile acids and bilirubin. Through their activity these transporters significantly affect the pharmacokinetics of many therapeutic agents impacting both therapeutic efficacy and the potential for drug-drug interactions. Their function ensures the proper uptake and processing of substrates within the liver contributing to the body's metabolic balance.

Pathways

OATP1B1 and OATP1B3 are integral components of the hepatic drug uptake pathway directly influencing the hepatic clearance of drugs. They play significant roles in the bile acid recycling pathway which maintains bile acid homeostasis. Both transporters interact with cytochrome P450 enzymes such as CYP3A4 to facilitate the metabolic processing of drugs within hepatocytes. Their coordinated action with these enzymes assists in the elimination of bile acids and other anions thereby supporting normal liver function and detoxification processes.

Alterations in OATP1B1 and OATP1B3 function associate with drug-induced liver injury and hyperbilirubinemia. Genetic polymorphisms in SLCO1B1 and SLCO1B3 can lead to altered transporter activity impacting drug disposition and increasing the risk of adverse drug reactions. A notable example is the association between SLCO1B1 variants and increased systemic exposure to statins which can result in statin-induced myopathy. Additionally conditions such as Rotor syndrome link to mutations in SLCO1B1 and SLCO1B3 leading to conjugated hyperbilirubinemia due to impaired hepatic uptake of bilirubin. These relationships highlight the clinical importance of OATP1B1 and OATP1B3 in pharmacogenomics and disease risk assessment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mediates the Na(+)-independent uptake of organic anions (PubMed : 10358072, PubMed : 15159445, PubMed : 17412826). Shows broad substrate specificity, can transport both organic anions such as bile acid taurocholate (cholyltaurine) and conjugated steroids (dehydroepiandrosterone 3-sulfate, 17-beta-glucuronosyl estradiol, and estrone 3-sulfate), as well as eicosanoids (prostaglandin E2, thromboxane B2, leukotriene C4, and leukotriene E4), and thyroid hormones (T4/L-thyroxine, and T3/3,3',5'-triiodo-L-thyronine) (PubMed : 10358072, PubMed : 10601278, PubMed : 10873595, PubMed : 11159893, PubMed : 12196548, PubMed : 12568656, PubMed : 15159445, PubMed : 15970799, PubMed : 16627748, PubMed : 17412826, PubMed : 19129463, PubMed : 26979622). Can take up bilirubin glucuronides from plasma into the liver, contributing to the detoxification-enhancing liver-blood shuttling loop (PubMed : 22232210). Involved in the clearance of endogenous and exogenous substrates from the liver (PubMed : 10358072, PubMed : 10601278). Transports coproporphyrin I and III, by-products of heme synthesis, and may be involved in their hepatic disposition (PubMed : 26383540). May contribute to regulate the transport of organic compounds in testes across the blood-testis-barrier (Probable). Can transport HMG-CoA reductase inhibitors (also known as statins), such as pravastatin and pitavastatin, a clinically important class of hypolipidemic drugs (PubMed : 10601278, PubMed : 15159445, PubMed : 15970799). May play an important role in plasma and tissue distribution of the structurally diverse chemotherapeutic drug methotrexate (PubMed : 23243220). May also transport antihypertension agents, such as the angiotensin-converting enzyme (ACE) inhibitor prodrug enalapril, and the highly selective angiotensin II AT1-receptor antagonist valsartan, in the liver (PubMed : 16624871, PubMed : 16627748). Shows a pH-sensitive substrate specificity towards prostaglandin E2 and T4 which may be ascribed to the protonation state of the binding site and leads to a stimulation of substrate transport in an acidic microenvironment (PubMed : 19129463). Hydrogencarbonate/HCO3(-) acts as the probable counteranion that exchanges for organic anions (PubMed : 19129463).
See full target information SLCO1B1

Additional targets

SLCO1B3
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Surgical case reports 2:89 PubMed27589984

2016

Central bisectionectomy for hepatocellular carcinoma in a patient with indocyanine green excretory defect associated with reduced expression of the liver transporter.

Applications

Unspecified application

Species

Unspecified reactive species

Shinya Imada,Tsuyoshi Kobayashi,Azusa Kitao,Osamu Matsui,Masakazu Hashimoto,Kentaro Ide,Kohei Ishiyama,Koji Arihiro,Hirotaka Tashiro,Hideki Ohdan

Biomolecules & therapeutics 23:400-6 PubMed26336578

2015

Role of miR-511 in the Regulation of OATP1B1 Expression by Free Fatty Acid.

Applications

WB

Species

Unspecified reactive species

Jin Fu Peng,Li Liu,Cheng Xian Guo,Shi Kun Liu,Xiao Ping Chen,Li Hua Huang,Hong Xiang,Zhi Jun Huang,Hong Yuan,Guo Ping Yang

International journal of clinical and experimental pathology 8:5252-62 PubMed26191226

2015

Down-regulation of OATP1B proteins correlates with hyperbilirubinemia in advanced cholestasis.

Applications

Unspecified application

Species

Unspecified reactive species

Eva Sticova,Alena Lodererova,Evita van de Steeg,Sona Frankova,Marek Kollar,Vera Lanska,Radana Kotalova,Tomas Dedic,Alfred H Schinkel,Milan Jirsa
View all publications
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Product promise

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