AB191412

Anti-OATP5A1 antibody [EPR14395(2)]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal OATP5A1 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

OATP5A1, SLC21A15, SLCO5A1, Solute carrier organic anion transporter family member 5A1, Organic anion transporter polypeptide-related protein 4, Solute carrier family 21 member 15, OATP-RP4, OATPRP4

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OATP5A1 antibody [EPR14395(2)] (AB191412)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OATP5A1 antibody [EPR14395(2)] (AB191412)

Immunohistochemical analysis of paraffin-embedded Human fetal kidney tissue labeling OATP5A1 using ab191412 at 1/100 dilution (10μg/mL). A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain : Hematoxylin. Inset image : negative control obtained using PBS instead of ab191412.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OATP5A1 antibody [EPR14395(2)] (AB191412)

Immunohistochemical analysis of paraffin-embedded Mouse fetal kidney tissue labeling OATP5A1 using ab191412 at 1/100 dilution (10 μg/mL). A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain : Hematoxylin. Inset image : negative control obtained using PBS instead of ab191412.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-OATP5A1 antibody [EPR14395(2)] (AB191412)

All lanes:

Western blot - Anti-OATP5A1 antibody [EPR14395(2)] (ab191412) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 20 µg

Lane 2:

T47D cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 92 kDa

false

Supplier Data

Western blot - Anti-OATP5A1 antibody [EPR14395(2)] (AB191412)

All lanes:

Western blot - Anti-OATP5A1 antibody [EPR14395(2)] (ab191412) at 1/10000 dilution

Lane 1:

Human fetal brain tissue lysate at 20 µg

Lane 2:

Human fetal heart tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 92 kDa

false

421 Alexa Fluor® 405

Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed
603 Alexa Fluor® 568

Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14395(2)

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OATP5A1 also known as Organic Anion Transporting Polypeptide 5A1 or SLCO5A1 is a member of the solute carrier organic anion transporter family. This protein helps transport a wide variety of molecules including drugs hormones and waste across cell membranes. It weighs approximately 87 kDa. Researchers have observed its expression in several tissues which include the brain heart and placenta. Its role as a transporter makes it important for maintaining cellular homeostasis by facilitating substance exchange within the body.

Biological function summary

OATP5A1 participates in the cellular uptake of various endogenous and exogenous substrates. Although not a part of a larger protein complex OATP5A1 works in concert with other transporters and cellular components to contribute to effective intracellular transport mechanisms. It regulates the concentration of substances within cells supporting key physiological processes such as hormone regulation and drug metabolism impacting how these substances are processed and utilized.

Pathways

OATP5A1 plays significant roles in two important biological pathways: drug metabolism and hormone regulation. The protein's activity influences drug disposition and pharmacokinetics interacting with proteins like cytochrome P450 enzymes along the way. Furthermore OATP5A1 helps drive hormone regulatory pathways alongside proteins such as transporters from the same SLCO family facilitating the appropriate biological responses necessary for maintaining health.

OATP5A1 has links to cancer and multi-drug resistance. Abnormal expression or dysfunction of this transporter can alter drug accumulation in cells affecting chemotherapy's effectiveness and potentially leading to treatment-resistant cancer types. Similarly in the context of drug resistance studies indicate its association with other transporter proteins like P-glycoprotein emphasizing its function in mediating how cells react to pharmacological treatments. Understanding OATP5A1's behavior better could therefore illuminate target pathways for overcoming treatment resistance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information SLCO5A1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular neuroscience 14:688050 PubMed34630032

2021

Tandem Mass Tag-Based Quantitative Proteomic Analysis Reveals Pathways Involved in Brain Injury Induced by Chest Exposure to Shock Waves.

Applications

Unspecified application

Species

Unspecified reactive species

Changci Tong,Peifang Cong,Ying Liu,Xiuyun Shi,Lin Shi,Shun Mao,Yan Zhao,Mingxiao Hou,Yunen Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-OATP5A1 antibody [EPR14395(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OATP5A1 antibody [EPR14395(2)] (AB191412)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OATP5A1 antibody [EPR14395(2)] (AB191412)

Western blot - Anti-OATP5A1 antibody [EPR14395(2)] (AB191412)

Western blot - Anti-OATP5A1 antibody [EPR14395(2)] (AB191412)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise