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AB223481

Anti-OAZ1 antibody

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(4 Publications)

Goat Polyclonal OAZ1 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications.

View Alternative Names

OAZ, OAZ1, Ornithine decarboxylase antizyme 1, AZ1, ODC-Az

1 Images
Western blot - Anti-OAZ1 antibody (AB223481)
  • WB

Supplier Data

Western blot - Anti-OAZ1 antibody (AB223481)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-OAZ1 antibody (ab223481) at 0.3 µg/mL

All lanes:

Human kidney lysate (in RIPA buffer) at 35 µg

Predicted band size: 25 kDa

Observed band size: 25 kDa

true

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab223481 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OAZ1 also known as Ornithine Decarboxylase Antizyme 1 is a regulatory protein with a mass of approximately 27 kDa. It functions to inhibit ornithine decarboxylase (ODC) an enzyme that plays a role in the biosynthesis of polyamines. These polyamines are important for cell growth and differentiation. OAZ1 is widely expressed in various tissues including liver kidney and brain indicating its broad regulatory role in cellular function.
Biological function summary

OAZ1 acts as an essential part of the polyamine homeostasis by regulating the degradation of ornithine decarboxylase. This regulation occurs through binding to ODC targeting it for degradation by the 26S proteasome and preventing its activity. OAZ1 does not form a larger protein complex but it plays an important role in controlling polyamine levels which are critical for many cellular functions such as DNA stabilization and transcription.

Pathways

OAZ1 integrates into the polyamine biosynthesis and catabolism pathways. Its inhibitory function on ODC plays a significant role in maintaining polyamine balance. This activity places OAZ1 in relationship with other proteins like antizyme inhibitor 1 (AZIN1) which modulates OAZ1 activity by binding and reducing its inhibitory effect on ODC. OAZ1's function connects with the broader network of protein catabolism pathways that regulate cellular proliferation.

OAZ1 holds significance in conditions related to polyamine dysregulation including cancer and neurological disorders. In cancer the overexpression of ODC can lead to excessive cell growth while OAZ1 helps in suppressing this by promoting ODC degradation. In neurodegenerative diseases altered polyamine metabolism involving OAZ1 may influence pathogenesis. Its interaction with ODC and AZIN1 factors into these disease processes highlighting OAZ1's role in maintaining cellular health in both normal and diseased states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ornithine decarboxylase (ODC) antizyme protein that negatively regulates ODC activity and intracellular polyamine biosynthesis and uptake in response to increased intracellular polyamine levels. Binds to ODC monomers, inhibiting the assembly of the functional ODC homodimer, and targets the monomers for ubiquitin-independent proteolytic destruction by the 26S proteasome (PubMed : 17900240, PubMed : 26305948, PubMed : 26443277). Triggers ODC degradation by inducing the exposure of a cryptic proteasome-interacting surface of ODC (PubMed : 26305948). Stabilizes AZIN2 by interfering with its ubiquitination (PubMed : 17900240). Also inhibits cellular uptake of polyamines by inactivating the polyamine uptake transporter. SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. Involved in the translocation of AZIN2 from ER-Golgi intermediate compartment (ERGIC) to the cytosol (PubMed : 12097147).
See full target information OAZ1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 15:1613458 PubMed40823069

2025

Polyamine metabolism related gene index prediction of prognosis and immunotherapy response in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ruoya Wang,Shouliang Cai,Qing Gao,Yidong Chen,Xue Han,Fangjian Shang,Chunyan Liang,Guolian Zhu,Bo Chen

Journal of experimental & clinical cancer research : CR 44:56 PubMed39962590

2025

AZIN1 level is increased in medulloblastoma and correlates with c-Myc activity and tumor phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Julie Sesen,Tyra Martinez,Sara Busatto,Larysa Poluben,Hassan Nassour,Caroline Stone,Karthik Ashok,Marsha A Moses,Edward R Smith,Aram Ghalali

Breast cancer research : BCR 23:65 PubMed34118960

2021

The small G-protein RalA promotes progression and metastasis of triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Katie A Thies,Matthew W Cole,Rachel E Schafer,Jonathan M Spehar,Dillon S Richardson,Sarah A Steck,Manjusri Das,Arthur W Lian,Alo Ray,Reena Shakya,Sue E Knoblaugh,Cynthia D Timmers,Michael C Ostrowski,Arnab Chakravarti,Gina M Sizemore,Steven T Sizemore

Biomolecules 11: PubMed34067619

2021

Characterising the Response of Human Breast Cancer Cells to Polyamine Modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Oluwaseun Akinyele,Heather M Wallace
View all publications
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