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Rabbit Polyclonal OAZ1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-OAZ1 antibody (AB224080), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OAZ1 antibody (AB224080), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information. Database link P54368

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IF
Human
Tested
Tested
Cow
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/20.00000 - 1/50.00000

Notes

-

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

0.25000-2.00000 µg/mL

Notes

Fixation/Permeabilization: PFA/Triton X-100.

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Ornithine decarboxylase (ODC) antizyme protein that negatively regulates ODC activity and intracellular polyamine biosynthesis and uptake in response to increased intracellular polyamine levels. Binds to ODC monomers, inhibiting the assembly of the functional ODC homodimer, and targets the monomers for ubiquitin-independent proteolytic destruction by the 26S proteasome (PubMed:17900240, PubMed:26305948, PubMed:26443277). Triggers ODC degradation by inducing the exposure of a cryptic proteasome-interacting surface of ODC (PubMed:26305948). Stabilizes AZIN2 by interfering with its ubiquitination (PubMed:17900240). Also inhibits cellular uptake of polyamines by inactivating the polyamine uptake transporter. SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. Involved in the translocation of AZIN2 from ER-Golgi intermediate compartment (ERGIC) to the cytosol (PubMed:12097147).

Alternative names

Recommended products

Rabbit Polyclonal OAZ1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information. Database link P54368
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

OAZ1 also known as Ornithine Decarboxylase Antizyme 1 is a regulatory protein with a mass of approximately 27 kDa. It functions to inhibit ornithine decarboxylase (ODC) an enzyme that plays a role in the biosynthesis of polyamines. These polyamines are important for cell growth and differentiation. OAZ1 is widely expressed in various tissues including liver kidney and brain indicating its broad regulatory role in cellular function.

Biological function summary

OAZ1 acts as an essential part of the polyamine homeostasis by regulating the degradation of ornithine decarboxylase. This regulation occurs through binding to ODC targeting it for degradation by the 26S proteasome and preventing its activity. OAZ1 does not form a larger protein complex but it plays an important role in controlling polyamine levels which are critical for many cellular functions such as DNA stabilization and transcription.

Pathways

OAZ1 integrates into the polyamine biosynthesis and catabolism pathways. Its inhibitory function on ODC plays a significant role in maintaining polyamine balance. This activity places OAZ1 in relationship with other proteins like antizyme inhibitor 1 (AZIN1) which modulates OAZ1 activity by binding and reducing its inhibitory effect on ODC. OAZ1's function connects with the broader network of protein catabolism pathways that regulate cellular proliferation.

Associated diseases and disorders

OAZ1 holds significance in conditions related to polyamine dysregulation including cancer and neurological disorders. In cancer the overexpression of ODC can lead to excessive cell growth while OAZ1 helps in suppressing this by promoting ODC degradation. In neurodegenerative diseases altered polyamine metabolism involving OAZ1 may influence pathogenesis. Its interaction with ODC and AZIN1 factors into these disease processes highlighting OAZ1's role in maintaining cellular health in both normal and diseased states.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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2 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-OAZ1 antibody (ab224080), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-OAZ1 antibody (ab224080)

    PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for OAZ1 (green) using ab224080 (4 μg/ml) in ICC/IF.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OAZ1 antibody (ab224080), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OAZ1 antibody (ab224080)

    Paraffin embedded human skeletal muscle tissue stained for OAZ1 with ab224080 (1/20 dilution) in immunohistochemical analysis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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