AB251554

Anti-Obestatin antibody [EPR19972] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal GHRL antibody. Carrier free. Suitable for WB, IHC-Fr, IHC-P and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

MTLRP, UNQ524/PRO1066, GHRL, Appetite-regulating hormone, Growth hormone secretagogue, Growth hormone-releasing peptide, Motilin-related peptide, Protein M46

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Obestatin antibody [EPR19972] - BSA and Azide free (AB251554)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Obestatin antibody [EPR19972] - BSA and Azide free (AB251554)

This data was developed using ab216575, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Obestatin with ab216575 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at a ready to use dilution.

Negative control : no staining on human colon [PMID : 18474938].

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Obestatin antibody [EPR19972] - BSA and Azide free (AB251554)

This data was developed using ab216575, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Obestatin with ab216575 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at a ready to use dilution.

Cytoplasmic staining on endocrine cells of human stomach is observed [PMID : 18474938].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at a ready to use dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Obestatin antibody [EPR19972] - BSA and Azide free (AB251554)

This data was developed using ab216575, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human duodenum tissue labeling Obestatin with ab216575 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at a ready to use dilution.

Cytoplasmic staining on endocrine cells of human duodenum is observed [PMID : 18474938].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Frozen sections) - Anti-Obestatin antibody [EPR19972] - BSA and Azide free (AB251554)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Obestatin antibody [EPR19972] - BSA and Azide free (AB251554)

This data was developed using ab216575, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse stomach tissue labeling Obestatin with ab216575 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Cytoplasmic staining on endocrine cells of mouse stomach is observed PMID : 18474938].

The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Obestatin antibody [EPR19972] - BSA and Azide free (AB251554)

This data was developed using ab216575, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Obestatin with ab216575 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at a ready to use dilution.

Cytoplasmic staining on endocrine cells of mouse stomach is observed [PMID : 18474938].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at a ready to use dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Obestatin antibody [EPR19972] - BSA and Azide free (AB251554)

This data was developed using ab216575, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Obestatin and Ghrelin are processed cleaved forms deriving from Appetite-regulating hormone. This product is specific to Obestatin.

Detecting Obestatin in endogenous tissues may require optimization.

All lanes:

Western blot - Anti-Obestatin antibody [EPR19972] (<a href='/en-us/products/primary-antibodies/obestatin-antibody-epr19972-ab216575'>ab216575</a>) at 1/1000 dilution

Lane 1:

GST and His-tagged human Appetite-regulating hormone, full length recombinant protein (aa1-117) at 0.005 µg

Lane 2:

His-tagged human Obestatin peptide (aa76-98)*5 at 0.005 µg

Lane 3:

His-tagged human Ghrelin peptide (aa24-51)*4 at 0.05 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 13 kDa

Observed band size: 13 kDa,38 kDa

false

Exposure time: 1s

Unconjugated

Anti-Obestatin antibody [EPR19972]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19972

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

IHC-P, WB, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20).", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab251554 is the carrier-free version of ab216575.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Obestatin also known as the obestatin hormone is a 23-amino acid peptide with a mass of approximately 2.5 kDa. Researchers discovered it as a cleavage product from the Ghrelin preprohormone. Obestatin is mainly expressed in the stomach and small intestine but one can also find it in other tissues such as the pancreas and adipose tissue. This peptide acts mechanically by binding to its receptor GPR39 although the binding affinity and downstream effects remain subjects of investigation.

Biological function summary

This peptide hormone interacts with several bodily processes mostly related to gastrointestinal functions and energy homeostasis. It does not form part of a larger protein complex but acts independently. Studies suggest it could play roles in reducing food intake and slowing down gastric emptying potentially contrasting the action of Ghrelin which stimulates hunger. Understanding its precise biological role remains complex with ongoing research needed to clarify its function fully.

Pathways

Several metabolic circuits include obestatin within their framework. It engages in pathways associated with energy balance and gut motility. In relation to the ghrelin axis specialists evaluate its potential modulatory effects given its origin from the same precursor. So far research indicates possible interplay with pathways influencing insulin release although the mechanistic details are not yet fully understood.

Preliminary research indicates obestatin might have connections to obesity and metabolic syndrome. Some scholars propose a potential protective effect against weight gain but findings often vary. Obestatin's relationship with the aforementioned diseases might involve its interaction with ghrelin highlighting a complex regulatory network that warrants further investigation. These insights could lead to novel therapeutic avenues for tackling metabolic disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ghrelin-27. Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR) (PubMed : 10604470). Induces the release of growth hormone from the pituitary (PubMed : 10604470). Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.. Ghrelin-28. Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR) (PubMed : 10604470). Induces the release of growth hormone from the pituitary (PubMed : 10604470). Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.. Obestatin. May be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility (By similarity).

See full target information GHRL

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell 39:1479-1496.e18 PubMed34653364

2021

Signatures of plasticity, metastasis, and immunosuppression in an atlas of human small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph M Chan,Álvaro Quintanal-Villalonga,Vianne Ran Gao,Yubin Xie,Viola Allaj,Ojasvi Chaudhary,Ignas Masilionis,Jacklynn Egger,Andrew Chow,Thomas Walle,Marissa Mattar,Dig V K Yarlagadda,James L Wang,Fathema Uddin,Michael Offin,Metamia Ciampricotti,Besnik Qeriqi,Amber Bahr,Elisa de Stanchina,Umesh K Bhanot,W Victoria Lai,Matthew J Bott,David R Jones,Arvin Ruiz,Marina K Baine,Yanyun Li,Natasha Rekhtman,John T Poirier,Tal Nawy,Triparna Sen,Linas Mazutis,Travis J Hollmann,Dana Pe'er,Charles M Rudin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Obestatin antibody [EPR19972] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Obestatin antibody [EPR19972] - BSA and Azide free (AB251554)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Obestatin antibody [EPR19972] - BSA and Azide free (AB251554)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Obestatin antibody [EPR19972] - BSA and Azide free (AB251554)

Immunohistochemistry (Frozen sections) - Anti-Obestatin antibody [EPR19972] - BSA and Azide free (AB251554)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Obestatin antibody [EPR19972] - BSA and Azide free (AB251554)

Western blot - Anti-Obestatin antibody [EPR19972] - BSA and Azide free (AB251554)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise