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AB204075

Anti-OBSL1 antibody

4

(1 Review)

|

(1 Publication)

Rabbit Polyclonal OBSL1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human OBSL1 aa 1250-1400.

View Alternative Names

KIAA0657, OBSL1, Obscurin-like protein 1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OBSL1 antibody (AB204075)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OBSL1 antibody (AB204075)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue, labeling OBSL1 with ab204075 at 1/50 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-OBSL1 antibody (AB204075)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-OBSL1 antibody (AB204075)

Immunofluorescence analysis of U-251 MG cells (PFA-fixed/Triton X-100 permeabilized) labeling OBSL1 with ab204075 at 4 μg/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human OBSL1 aa 1250-1400. The exact immunogen used to generate this antibody is proprietary information.

O75147

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target OBSL1 also known as Obscurin-like 1 is a protein mechanically involved in the cellular architecture. It possesses a mass of approximately 189 kDa. OBSL1 is known for its expression in various tissues notably in muscle and heart. It helps maintain structural integrity by interacting with cytoskeletal proteins. This protein features multiple domains that enable its structural functions and protein-protein interactions.
Biological function summary

The role of Obscurin-like 1 extends to maintaining cytoskeletal stability. It is involved in muscle and skeletal systems contributing to the organization of myofibrils. OBSL1 functions as part of a protein complex that helps connect the contractile apparatus to the sarcolemma. It interacts extensively with other cytoskeletal elements coordinating the proper alignment and spacing of muscle fibers which is important for muscle function.

Pathways

The interactions involving Obscurin-like 1 pertain to the signaling mechanisms that regulate muscle development and repair. The protein participates in pathways such as the integrin signaling pathway and the regulation of the cytoskeleton. It shows association with proteins like titin and ankyrin playing a critical role in the assembly and maintenance of muscle cells. These interactions facilitate cellular response to mechanical stress and help organize sarcomeres.

Research links Obscurin-like 1 with muscular disorders and growth deficiencies. Mutations or dysregulation in OBSL1 have connections to conditions like 3M syndrome which affects growth hormone signaling pathways and results in growth retardation. The protein associates with CUL7 which is involved in ubiquitination processes. Alterations in OBSL1 can disrupt normal cellular function highlighting its importance in health and disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Core component of the 3M complex, a complex required to regulate microtubule dynamics and genome integrity. It is unclear how the 3M complex regulates microtubules, it could act by controlling the level of a microtubule stabilizer (PubMed : 24793695, PubMed : 24793696). Acts as a regulator of the Cul7-RING(FBXW8) ubiquitin-protein ligase, playing a critical role in the ubiquitin ligase pathway that regulates Golgi morphogenesis and dendrite patterning in brain. Required to localize CUL7 to the Golgi apparatus in neurons.
See full target information OBSL1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Functional & integrative genomics 24:38 PubMed38376551

2024

DKC1 aggravates gastric cancer cell migration and invasion through up-regulating the expression of TNFAIP6.

Applications

Unspecified application

Species

Unspecified reactive species

Huihua Chen,Yibo Wu,Yancheng Jiang,Zixuan Chen,Tingjin Zheng
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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