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Rabbit Polyclonal OBSL1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Obscurin-like protein 1.

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Images

Western blot - Anti-OBSL1 antibody (AB224588), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OBSL1 antibody (AB224588), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human Obscurin-like protein 1. Database link O75147

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/500.00000 - 1/5000.00000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/20.00000 - 1/200.00000

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Core component of the 3M complex, a complex required to regulate microtubule dynamics and genome integrity. It is unclear how the 3M complex regulates microtubules, it could act by controlling the level of a microtubule stabilizer (PubMed:24793695, PubMed:24793696). Acts as a regulator of the Cul7-RING(FBXW8) ubiquitin-protein ligase, playing a critical role in the ubiquitin ligase pathway that regulates Golgi morphogenesis and dendrite patterning in brain. Required to localize CUL7 to the Golgi apparatus in neurons.

Alternative names

Recommended products

Rabbit Polyclonal OBSL1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Obscurin-like protein 1.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Human Obscurin-like protein 1. Database link O75147
Purification technique

Affinity purification Protein G

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The target OBSL1 also known as Obscurin-like 1 is a protein mechanically involved in the cellular architecture. It possesses a mass of approximately 189 kDa. OBSL1 is known for its expression in various tissues notably in muscle and heart. It helps maintain structural integrity by interacting with cytoskeletal proteins. This protein features multiple domains that enable its structural functions and protein-protein interactions.

Biological function summary

The role of Obscurin-like 1 extends to maintaining cytoskeletal stability. It is involved in muscle and skeletal systems contributing to the organization of myofibrils. OBSL1 functions as part of a protein complex that helps connect the contractile apparatus to the sarcolemma. It interacts extensively with other cytoskeletal elements coordinating the proper alignment and spacing of muscle fibers which is important for muscle function.

Pathways

The interactions involving Obscurin-like 1 pertain to the signaling mechanisms that regulate muscle development and repair. The protein participates in pathways such as the integrin signaling pathway and the regulation of the cytoskeleton. It shows association with proteins like titin and ankyrin playing a critical role in the assembly and maintenance of muscle cells. These interactions facilitate cellular response to mechanical stress and help organize sarcomeres.

Associated diseases and disorders

Research links Obscurin-like 1 with muscular disorders and growth deficiencies. Mutations or dysregulation in OBSL1 have connections to conditions like 3M syndrome which affects growth hormone signaling pathways and results in growth retardation. The protein associates with CUL7 which is involved in ubiquitination processes. Alterations in OBSL1 can disrupt normal cellular function highlighting its importance in health and disease.

Product promise

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2 product images

  • Western blot - Anti-OBSL1 antibody (ab224588), expandable thumbnail

    Western blot - Anti-OBSL1 antibody (ab224588)

    All lanes: Western blot - Anti-OBSL1 antibody (ab224588) at 1/500 dilution

    Lane 1: HepG2 (human liver hepatocellular carcinoma cell line) cell lysate

    Lane 2: MCF7 (human breast adenocarcinoma cell line) cell lysate

    Lane 3: A549 (human lung carcinoma cell line) cell lysate

    Secondary

    All lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 207 kDa, 51 kDa

    Observed band size: 207 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OBSL1 antibody (ab224588), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OBSL1 antibody (ab224588)

    Paraffin-embedded human placenta tissue stained for OBSL1 using ab224588 at 1/100 dilution in immunohistochemical analysis.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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