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AB224588

Anti-OBSL1 antibody

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(1 Publication)

Rabbit Polyclonal OBSL1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human OBSL1.

View Alternative Names

KIAA0657, OBSL1, Obscurin-like protein 1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OBSL1 antibody (AB224588)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OBSL1 antibody (AB224588)

Paraffin-embedded human placenta tissue stained for OBSL1 using ab224588 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-OBSL1 antibody (AB224588)
  • WB

Supplier Data

Western blot - Anti-OBSL1 antibody (AB224588)

All lanes:

Western blot - Anti-OBSL1 antibody (ab224588) at 1/500 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) cell lysate

Lane 2:

MCF7 (human breast adenocarcinoma cell line) cell lysate

Lane 3:

A549 (human lung carcinoma cell line) cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 207 kDa,51 kDa

Observed band size: 207 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human OBSL1.

O75147

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target OBSL1 also known as Obscurin-like 1 is a protein mechanically involved in the cellular architecture. It possesses a mass of approximately 189 kDa. OBSL1 is known for its expression in various tissues notably in muscle and heart. It helps maintain structural integrity by interacting with cytoskeletal proteins. This protein features multiple domains that enable its structural functions and protein-protein interactions.
Biological function summary

The role of Obscurin-like 1 extends to maintaining cytoskeletal stability. It is involved in muscle and skeletal systems contributing to the organization of myofibrils. OBSL1 functions as part of a protein complex that helps connect the contractile apparatus to the sarcolemma. It interacts extensively with other cytoskeletal elements coordinating the proper alignment and spacing of muscle fibers which is important for muscle function.

Pathways

The interactions involving Obscurin-like 1 pertain to the signaling mechanisms that regulate muscle development and repair. The protein participates in pathways such as the integrin signaling pathway and the regulation of the cytoskeleton. It shows association with proteins like titin and ankyrin playing a critical role in the assembly and maintenance of muscle cells. These interactions facilitate cellular response to mechanical stress and help organize sarcomeres.

Research links Obscurin-like 1 with muscular disorders and growth deficiencies. Mutations or dysregulation in OBSL1 have connections to conditions like 3M syndrome which affects growth hormone signaling pathways and results in growth retardation. The protein associates with CUL7 which is involved in ubiquitination processes. Alterations in OBSL1 can disrupt normal cellular function highlighting its importance in health and disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Core component of the 3M complex, a complex required to regulate microtubule dynamics and genome integrity. It is unclear how the 3M complex regulates microtubules, it could act by controlling the level of a microtubule stabilizer (PubMed : 24793695, PubMed : 24793696). Acts as a regulator of the Cul7-RING(FBXW8) ubiquitin-protein ligase, playing a critical role in the ubiquitin ligase pathway that regulates Golgi morphogenesis and dendrite patterning in brain. Required to localize CUL7 to the Golgi apparatus in neurons.
See full target information OBSL1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 41:111571 PubMed36323262

2022

Nuclear stabilization of p53 requires a functional nucleolar surveillance pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine M Hannan,Priscilla Soo,Mei S Wong,Justine K Lee,Nadine Hein,Perlita Poh,Kira D Wysoke,Tobias D Williams,Christian Montellese,Lorey K Smith,Sheren J Al-Obaidi,Lorena Núñez-Villacís,Megan Pavy,Jin-Shu He,Kate M Parsons,Karagh E Loring,Tess Morrison,Jeannine Diesch,Gaetan Burgio,Rita Ferreira,Zhi-Ping Feng,Cathryn M Gould,Piyush B Madhamshettiwar,Johan Flygare,Thomas J Gonda,Kaylene J Simpson,Ulrike Kutay,Richard B Pearson,Christoph Engel,Nicholas J Watkins,Ross D Hannan,Amee J George
View all publications
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Product promise

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