AB222691

Anti-Occludin antibody

Name: Anti-Occludin antibody (ab222691) | Abcam
Brand: Abcam Limited
SKU: AB222691
Price: 515 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(29 Publications)

Rabbit Polyclonal Occludin antibody. Suitable for Flow Cyt, WB and reacts with Human, Mouse samples. Cited in 29 publications. Immunogen corresponding to Synthetic Peptide within Mouse Ocln aa 450 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Occludin, OCLN

3 Images

Flow Cytometry - Anti-Occludin antibody (AB222691)

Flow Cyt

Unknown

Flow Cytometry - Anti-Occludin antibody (AB222691)

Flow Cytometry analysis of 293T cells labelling Occludin with ab222691 at 1/100 for 30 minutes compared to control cells (dark blue), secondary only (light blue) and isotype control (orange)

Supplier Data

Western blot - Anti-Occludin antibody (AB222691)

All lanes:

Western blot - Anti-Occludin antibody (ab222691) at 1/300 dilution

All lanes:

A549 cell lysate

Secondary

All lanes:

Goat Anti-Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution

Predicted band size: 59 kDa

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Western blot - Anti-Occludin antibody (AB222691)

All lanes:

Western blot - Anti-Occludin antibody (ab222691) at 1/300 dilution

All lanes:

Mouse kidney tissue lysate

Secondary

All lanes:

A conjugated secondary antibody at 1/5000 dilution

Predicted band size: 59 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, Flow Cyt

applications

Immunogen

Synthetic Peptide within Mouse Ocln aa 450 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q61146

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Occludin is a protein that plays an important mechanical role in forming tight junctions which are specialized connections between neighboring cell membranes. Alternative names for this target include occludin-1. The occludin protein has a molecular weight of approximately 65 kDa. It is expressed in a variety of tissues but levels tend to be high in epithelial and endothelial cells where tight junctions are essential for barrier functions. Occludin acts as integral membrane protein contributing to the complex structures that seal spaces between cells.

Biological function summary

Occludin serves as an important component in maintaining cell-to-cell adhesion within tight junction complexes. It interacts with other tight junction proteins such as claudins and junctional adhesion molecules to regulate paracellular permeability. These interactions help maintain selective barrier properties of epithelial and endothelial layers preventing the passage of large molecules and pathogens while allowing passage of ions and small molecules. Occludin stabilizes the tight junctions by interacting with the cytoskeleton and signaling proteins that are important for tight junction assembly and maintenance.

Pathways

Occludin functions prominently in the regulation of tight junction integrity and barrier function. It participates in pathways such as the tight junction signaling pathway and the paracellular transport pathway. Occludin interacts with ZO-1 a scaffolding protein bridging its interaction with the actin cytoskeleton which supports the structural organization and function of tight junctions. This connectivity allows for regulation of the permeability and transport across the epithelial barrier.

Disruptions in occludin function are connected to conditions such as inflammatory bowel disease (IBD) and some cancers. In IBD abnormal occludin expression can compromise intestinal barrier function leading to increased intestinal permeability and an inflammatory response. In certain cancers loss of occludin expression is linked to increased invasiveness and metastasis due to weakened cell adhesion. The dysfunction of proteins like ZO-1 which interact with occludin in these pathways also contributes to these pathological states highlighting the importance of occludin's regulatory roles in maintaining cellular barrier integrity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.. (Microbial infection) Acts as a coreceptor for hepatitis C virus (HCV) in hepatocytes.

See full target information OCLN

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Publications (29)

Recent publications for all applications. Explore the full list and refine your search

Biological procedures online 26:27 PubMed39187810

2024

Effects and Mechanisms of the Xianhecao-Huanglian Drug Pair on Autophagy-Mediated Intervention in Acute Inflammatory Bowel Disease via the JAK2/STAT3 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yaping He,Xinling Shen,Haiyan Peng

PloS one 19:e0303423 PubMed38885258

2024

Probiotic LB101 alleviates dry eye in mice by suppressing matrix metalloproteinase-9 expression through the regulation of gut microbiota-involved NF-κB signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyang Ma,Yoon-Jung Shin,Soo-Won Yun,Seok Won Jang,Seung-Won Han,Dong-Hyun Kim

Acta pharmaceutica Sinica. B 14:1827-1844 PubMed38572103

2024

A mix & act liposomes of phospholipase A2-phosphatidylserine for acute brain detoxification by blood‒brain barrier selective-opening.

Applications

Unspecified application

Species

Unspecified reactive species

Zinan Zhang,Wenbin Cao,Huanchun Xing,Shuai Guo,Lijuan Huang,Lin Wang,Xin Sui,Kui Lu,Yuan Luo,Yongan Wang,Jun Yang

Animals : an open access journal from MDPI 14: PubMed38473174

2024

The Metabolic Pathway of Bile Secretion Is Vulnerable to Exposure in Porcine Intestinal Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jiajia Cai,Xiaolei Chen,Chao Xu,Xiaoyang Zhu,Haifei Wang,Shenglong Wu,Demin Cai,Hairui Fan

Pharmaceutical biology 62:53-61 PubMed38108311

2023

Berberine decreases S100B generation to regulate gut vascular barrier permeability in mice with burn injury.

Applications

Unspecified application

Species

Unspecified reactive species

Aiwen Feng,Shaosheng Su,Cheng Li,Yutian Kang,Jiasheng Qiu,Jun Zhou

International journal of biological sciences 19:4360-4375 PubMed37781034

2023

Activation of P2Y1R impedes intestinal mucosa repair during colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuchan Huan,Jiao Peng,Yaoyao Chang,Qiansheng Zhang,Tianhang Xing,Danling Jiang,Wenke Chen,Xiangchun Shen,Zhaoxiang Bian,Haitao Xiao

Heliyon 9:e18244 PubMed37519691

2023

Fuzi polysaccharides improve immunity in immunosuppressed mouse models by regulating gut microbiota composition.

Applications

Unspecified application

Species

Unspecified reactive species

Ran Tu,Cheng Zhou,Wenfeng Huang,Zhengping Feng,Qiufang Zhao,Xiaofei Shi,Langjun Cui,Keke Chen

Chemistry & biodiversity 20:e202300572 PubMed37218365

2023

Picroside III, an Active Ingredient of Picrorhiza scrophulariiflora, Ameliorates Experimental Colitis by Protecting Intestinal Barrier Integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuchan Huan,Zhengxian Gao,Peigen Wu,Yingdi Zhang,Haitao Xiao,Jiao Peng

Frontiers in pharmacology 14:1163694 PubMed37089915

2023

Vitamin C and vitamin D alleviate metabolic-associated fatty liver disease by regulating the gut microbiota and bile acid metabolism the gut-liver axis.

Applications

Unspecified application

Species

Unspecified reactive species

Qingling Chen,Lili Zhao,Ling Mei,Xiaotong Zhao,Ping Han,Jie Liu,Chao Meng,Ruifang Li,Rui Zhong,Kai Wang,Jia Li

Nutrients 14: PubMed36364738

2022

Co-Cultures of and Enhance Mucosal Barrier by Modulating Gut Microbiota-Derived Short-Chain Fatty Acids.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengjun Xie,Meng Li,Mengqi Qian,Zhiren Yang,Xinyan Han

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Occludin antibody

Flow Cytometry - Anti-Occludin antibody (AB222691)

Western blot - Anti-Occludin antibody (AB222691)

Western blot - Anti-Occludin antibody (AB222691)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (29)

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