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AB235986

Anti-Occludin antibody

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(5 Publications)

Rabbit Polyclonal Occludin antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human OCLN aa 250-450.

View Alternative Names

Occludin, OCLN

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody (AB235986)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody (AB235986)

Paraffin-embedded human colon cancer tissue stained for Occludin using ab235986 at 1/400 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

Immunocytochemistry/ Immunofluorescence - Anti-Occludin antibody (AB235986)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Occludin antibody (AB235986)

4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells stained for Occludin (green) using ab235986 at 1/133 dilution in ICC/IF, followed by an Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.

Western blot - Anti-Occludin antibody (AB235986)
  • WB

Supplier Data

Western blot - Anti-Occludin antibody (AB235986)

All lanes:

Western blot - Anti-Occludin antibody (ab235986) at 1/500 dilution

All lanes:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 59 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human OCLN aa 250-450. The exact immunogen used to generate this antibody is proprietary information.

Q16625

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Occludin is a protein that plays an important mechanical role in forming tight junctions which are specialized connections between neighboring cell membranes. Alternative names for this target include occludin-1. The occludin protein has a molecular weight of approximately 65 kDa. It is expressed in a variety of tissues but levels tend to be high in epithelial and endothelial cells where tight junctions are essential for barrier functions. Occludin acts as integral membrane protein contributing to the complex structures that seal spaces between cells.
Biological function summary

Occludin serves as an important component in maintaining cell-to-cell adhesion within tight junction complexes. It interacts with other tight junction proteins such as claudins and junctional adhesion molecules to regulate paracellular permeability. These interactions help maintain selective barrier properties of epithelial and endothelial layers preventing the passage of large molecules and pathogens while allowing passage of ions and small molecules. Occludin stabilizes the tight junctions by interacting with the cytoskeleton and signaling proteins that are important for tight junction assembly and maintenance.

Pathways

Occludin functions prominently in the regulation of tight junction integrity and barrier function. It participates in pathways such as the tight junction signaling pathway and the paracellular transport pathway. Occludin interacts with ZO-1 a scaffolding protein bridging its interaction with the actin cytoskeleton which supports the structural organization and function of tight junctions. This connectivity allows for regulation of the permeability and transport across the epithelial barrier.

Disruptions in occludin function are connected to conditions such as inflammatory bowel disease (IBD) and some cancers. In IBD abnormal occludin expression can compromise intestinal barrier function leading to increased intestinal permeability and an inflammatory response. In certain cancers loss of occludin expression is linked to increased invasiveness and metastasis due to weakened cell adhesion. The dysfunction of proteins like ZO-1 which interact with occludin in these pathways also contributes to these pathological states highlighting the importance of occludin's regulatory roles in maintaining cellular barrier integrity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.. (Microbial infection) Acts as a coreceptor for hepatitis C virus (HCV) in hepatocytes.
See full target information OCLN
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurobiology 61:1936-1952 PubMed37819429

2023

Teriflunomide Promotes Blood-Brain Barrier Integrity by Upregulating Claudin-1 via the Wnt/β-catenin Signaling Pathway in Multiple Sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yipeng Zhao,Chen Chen,Xiuqing Xiao,Ling Fang,Xi Cheng,Yanyu Chang,Fuhua Peng,Jingqi Wang,Shishi Shen,Shilin Wu,Yiying Huang,Wei Cai,Linli Zhou,Wei Qiu

Cancer cell international 22:187 PubMed35568859

2022

Integrative analysis identifies CXCL11 as an immune-related prognostic biomarker correlated with cell proliferation and immune infiltration in multiple myeloma microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Huizhong Wang,Ruonan Shao,Wenjian Liu,Shumei Peng,Shenrui Bai,Bibo Fu,Congling Zhao,Yue Lu

Frontiers in physiology 12:637790 PubMed33868003

2021

The Degradation of Airway Epithelial Tight Junctions in Asthma Under High Airway Pressure Is Probably Mediated by Piezo-1.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Zhou,Xiang-Dong Zhou,Rui Xu,Xian-Zhi Du,Qi Li,Bin Li,Guo-Yue Zhang,Ling-Xiu Chen,Juliy M Perelman,Victor P Kolosov

Frontiers in immunology 11:2101 PubMed32983166

2020

Intestinal Barrier Breakdown and Mucosal Microbiota Disturbance in Neuromyelitis Optical Spectrum Disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Chunping Cui,Sha Tan,Li Tao,Junli Gong,Yanyu Chang,Yuge Wang,Ping Fan,Dan He,Yiwen Ruan,Wei Qiu

Cell biology international 44:1681-1690 PubMed32281710

2020

Knockdown of ghrelin-O-acyltransferase attenuates colitis through the modulation of inflammatory factors and tight junction proteins in the intestinal epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Peiying Tian,Xiaolan Lu,Nuyun Jin,Jianping Shi
View all publications
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