Anti-Occludin antibody [EPR20992] is a rabbit recombinant monoclonal antibody that is used to detect Occludin in Flow cytometry (Intra), ICC/IF, IHC-Fr, IHC-P, IP, Western blot. Suitable for Dog, Human, Mouse, Rat samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
- Specificity confirmed with OCLN knockout cell line validation
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- Antibody clone EPR20992 is cited in over 330 publications
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
IP | IHC-Fr | WB | ICC/IF | IHC-P | Flow Cyt (Intra) | |
---|---|---|---|---|---|---|
Human | Tested | Tested | Tested | Tested | Tested | Tested |
Mouse | Expected | Expected | Tested | Not recommended | Tested | Expected |
Rat | Expected | Expected | Tested | Not recommended | Tested | Expected |
Dog | Expected | Expected | Tested | Tested | Expected | Expected |
Recombinant full length protein - Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/40 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Dog | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/200 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Dog | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Human | Dilution info 1/1000 | Notes Please upload more of the lysate or use lower dilution of ab216327 when testing bEnd.3. Occludin contains multiple isoforms, and the molecular weight range of different isoforms varies greatly (8kDa~59kDa). In addition, there are various post-translational modifications such as ubiquitination and proteolytic cleavage, so the band sizes and number of bands detected in different samples may vary. (PMID: 18647175, PMID: 19821483, PMID: 19457074, PMID: 11782481, PMID: 22083955). |
Species Mouse | Dilution info 1/1000 | Notes Please upload more of the lysate or use lower dilution of ab216327 when testing bEnd.3. Occludin contains multiple isoforms, and the molecular weight range of different isoforms varies greatly (8kDa~59kDa). In addition, there are various post-translational modifications such as ubiquitination and proteolytic cleavage, so the band sizes and number of bands detected in different samples may vary. (PMID: 18647175, PMID: 19821483, PMID: 19457074, PMID: 11782481, PMID: 22083955). Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 | Notes Please upload more of the lysate or use lower dilution of ab216327 when testing bEnd.3. Occludin contains multiple isoforms, and the molecular weight range of different isoforms varies greatly (8kDa~59kDa). In addition, there are various post-translational modifications such as ubiquitination and proteolytic cleavage, so the band sizes and number of bands detected in different samples may vary. (PMID: 18647175, PMID: 19821483, PMID: 19457074, PMID: 11782481, PMID: 22083955). |
Species Human | Dilution info 1/1000 | Notes Please upload more of the lysate or use lower dilution of ab216327 when testing bEnd.3. Occludin contains multiple isoforms, and the molecular weight range of different isoforms varies greatly (8kDa~59kDa). In addition, there are various post-translational modifications such as ubiquitination and proteolytic cleavage, so the band sizes and number of bands detected in different samples may vary. (PMID: 18647175, PMID: 19821483, PMID: 19457074, PMID: 11782481, PMID: 22083955). |
Species Dog | Dilution info 1/1000 | Notes Please upload more of the lysate or use lower dilution of ab216327 when testing bEnd.3. Occludin contains multiple isoforms, and the molecular weight range of different isoforms varies greatly (8kDa~59kDa). In addition, there are various post-translational modifications such as ubiquitination and proteolytic cleavage, so the band sizes and number of bands detected in different samples may vary. (PMID: 18647175, PMID: 19821483, PMID: 19457074, PMID: 11782481, PMID: 22083955). |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 | Notes This antibody is not suitable for ICC in mouse or rat species. |
Species Dog | Dilution info 1/100 | Notes This antibody is not suitable for ICC in mouse or rat species. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes This antibody is not suitable for ICC in mouse or rat species. |
Species Rat | Dilution info - | Notes This antibody is not suitable for ICC in mouse or rat species. |
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/200 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/200 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/200 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Dog | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/60 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Dog | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
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May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. (Microbial infection) Acts as a coreceptor for hepatitis C virus (HCV) in hepatocytes.
Occludin, OCLN
Anti-Occludin antibody [EPR20992] is a rabbit recombinant monoclonal antibody that is used to detect Occludin in Flow cytometry (Intra), ICC/IF, IHC-Fr, IHC-P, IP, Western blot. Suitable for Dog, Human, Mouse, Rat samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
- Specificity confirmed with OCLN knockout cell line validation
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- Antibody clone EPR20992 is cited in over 330 publications
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
This antibody is not suitable for ICC in mouse or rat species or for any testing rat intestinal tissues.
FURTHER INFORMATION ON SPECIFICITY (Chinese Version) available under the support & downloads section.
Anti-Occludin antibody [EPR20992] (ab216327) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-Fr, IHC-P, IP and WB.
Anti-Occludin antibody [EPR20992] (ab216327) was first used in a scientific publication in 2018 and has been cited over 237 times in peer reviewed journals. It's performance in Western blot in human, mouse and rat samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-Occludin antibody [EPR20992] (ab216327) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-Occludin antibody [EPR20992] (ab216327) has been confirmed by Western Blot testing in Occludin knockout HAP1 cells.
Anti-Occludin antibody [EPR20992] (ab216327) has 8 independent reviews from customers.
Anti-Occludin antibody [EPR20992] (ab216327) specifically detects Occludin (UniProt ID: Q16625; Molecular weight: 60kDa) and is sold in a convenient trial size to enable initial testing (10 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Conjugation-ready, carrier free format available for antibody clone EPR20992 - Anti-Occludin antibody [EPR20992] - BSA and Azide free ab224526.
Occludin is a tight junction protein that plays a crucial role in maintaining the integrity of epithelial and endothelial barriers. In oncology, its downregulation is associated with increased tumorigenic and metastatic potential in cancers such as lung carcinoma, making it a potential prognostic biomarker and therapeutic target.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Occludin is a protein that plays an important mechanical role in forming tight junctions which are specialized connections between neighboring cell membranes. Alternative names for this target include occludin-1. The occludin protein has a molecular weight of approximately 65 kDa. It is expressed in a variety of tissues but levels tend to be high in epithelial and endothelial cells where tight junctions are essential for barrier functions. Occludin acts as integral membrane protein contributing to the complex structures that seal spaces between cells.
Occludin serves as an important component in maintaining cell-to-cell adhesion within tight junction complexes. It interacts with other tight junction proteins such as claudins and junctional adhesion molecules to regulate paracellular permeability. These interactions help maintain selective barrier properties of epithelial and endothelial layers preventing the passage of large molecules and pathogens while allowing passage of ions and small molecules. Occludin stabilizes the tight junctions by interacting with the cytoskeleton and signaling proteins that are important for tight junction assembly and maintenance.
Occludin functions prominently in the regulation of tight junction integrity and barrier function. It participates in pathways such as the tight junction signaling pathway and the paracellular transport pathway. Occludin interacts with ZO-1 a scaffolding protein bridging its interaction with the actin cytoskeleton which supports the structural organization and function of tight junctions. This connectivity allows for regulation of the permeability and transport across the epithelial barrier.
Disruptions in occludin function are connected to conditions such as inflammatory bowel disease (IBD) and some cancers. In IBD abnormal occludin expression can compromise intestinal barrier function leading to increased intestinal permeability and an inflammatory response. In certain cancers loss of occludin expression is linked to increased invasiveness and metastasis due to weakened cell adhesion. The dysfunction of proteins like ZO-1 which interact with occludin in these pathways also contributes to these pathological states highlighting the importance of occludin's regulatory roles in maintaining cellular barrier integrity.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Occludin with ab216327 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on human colon is observed (PMID: 24268521). Counter stained with Hematoxylin.
Secondary antibody only control: Rabbit specific IHC polymer detection kit HRP/DAB ab209101 (Rabbit specific IHC polymer detection kit HRP/DAB).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
All lanes: Western blot - Anti-Occludin antibody [EPR20992] (ab216327) at 1/1000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 40 µg
Lane 2: OCLN (Occludin) knockout HAP1 whole cell lysate at 40 µg
Lane 3: HeLa whole cell lysate (Low Occludin expression) at 20 µg
Lane 4: HepG2 whole cell lysate lysate (High Occludin expression) at 20 µg
Predicted band size: 59 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Occludin antibody [EPR20992] (ab216327) at 1/1000 dilution
Lane 1: Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: bEnd.3 (Mouse brain endothelioma) whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution
Predicted band size: 59 kDa
Observed band size: 65 kDa
Exposure time: 60s
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Caco-2 (human colorectal adenocarcinoma cell line) cells labeling Occludin with ab216327 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membrane staining on Caco-2 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Occludin antibody [EPR20992] (ab216327) at 1/1000 dilution
Lane 1: Mouse colon tissue lysate at 20 µg
Lane 2: Mouse brain tissue lysate at 20 µg
Lane 3: Mouse lung tissue lysate at 20 µg
Lane 4: Rat colon tissue lysate at 20 µg
Lane 5: Rat brain tissue lysate at 20 µg
Lane 6: Rat lung tissue lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution
Predicted band size: 59 kDa
Observed band size: 65 kDa
Exposure time: 100s
Occludin was immunoprecipitated from 0.35 mg of Caco-2 (human colorectal adenocarcinoma cell line) whole cell lysate with ab216327 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab216327 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution
Lane 1: Caco-2 whole cell lysate 10 μg (Input).
Lane 2: ab216327 IP in Caco-2 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab216327 in Caco-2 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
The molecular weight observed is consistent with what has been described in the literature (PMID: 18647175, PMID: 19821483).
All lanes: Immunoprecipitation - Anti-Occludin antibody [EPR20992] (ab216327)
Predicted band size: 59 kDa
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Occludin with ab216327 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on distal tubules of mouse kidney is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Rabbit specific IHC polymer detection kit HRP/DAB ab209101 (Rabbit specific IHC polymer detection kit HRP/DAB).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Occludin with ab216327 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on distal tubules of rat kidney is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Rabbit specific IHC polymer detection kit HRP/DAB ab209101 (Rabbit specific IHC polymer detection kit HRP/DAB).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemical analysis of paraffin-embedded human breast tissue labeling Occludin with ab216327 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on human breast is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Rabbit specific IHC polymer detection kit HRP/DAB ab209101 (Rabbit specific IHC polymer detection kit HRP/DAB).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDCK (canine kidney cell line) cells labeling Occludin with ab216327 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membrane staining on MDCK (NBL-2) cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Caco-2 (human colorectal adenocarcinoma cell line) cell line labeling Occludin with ab216327 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
Tissue Microarrays stained for Anti-Occludin antibody [EPR20992] using ab216327 in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negaive (cross mark) staining per sample type tested. The sections were incubated with ab216327 at 4°C overnight used at 1:2000 dilution (1.05 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Counterstain was Hematoxylin.
Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Occludin contains multiple isoforms, and the molecular weight range of different isoforms varies greatly (8kDa~59kDa). In addition, there are various post-translational modifications such as ubiquitination and proteolytic cleavage, so the band sizes and number of bands detected in different samples may vary. (PMID: 18647175, PMID: 19821483, PMID: 19457074, PMID: 11782481, PMID: 22083955)
Blocking and Diluting buffer and concentration: 5% NFDM /TBST
Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a GAPDH loading control.
All lanes: Western blot - Anti-Occludin antibody [EPR20992] (ab216327) at 1/1000 dilution
Lane 1: Mouse colon tissue lysate at 20 µg
Lane 2: Mouse small intestine tissue lysate at 20 µg
Lane 3: Mouse ileum tissue lysate at 20 µg
Lane 4: Mouse liver tissue lysate at 20 µg
Lane 5: Mouse Kidney tissue lysate at 20 µg
Lane 6: Human colon tissue lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 16-60 kDa
Exposure time: 100s
For cells express relatively low level of Occludin, we suggest optimizing experimental protocols (increasing lysate amount, using lower dilution or higher sensitivity ECL substrate) to improve results.
Blocking and Diluting buffer and concentration: 5% NFDM /TBST
Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a GAPDH loading control.
All lanes: Western blot - Anti-Occludin antibody [EPR20992] (ab216327) at 1/1000 dilution
Lane 1: Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: DLD-1(human colorectal adenocarcinoma cell) whole cell lysate at 20 µg
Lane 3: A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 5: HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 16-60 kDa
Exposure time: 20s
Immunohistochemical analysis of paraffin-embedded Rat colon labeling Occludin with ab216327 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Positive staining on Rat colon. The section was incubated with ab216327 at 4°C overnight. The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Immunohistochemical analysis of paraffin-embedded Mouse colon labeling Occludin with ab216327 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Positive staining on Mouse colon. The section was incubated with ab216327 at 4°C overnight. The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Immunohistochemical analysis of paraffin-embedded Human colon labeling Occludin with ab216327 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Positive staining on human colon. The section was incubated with ab216327 at 4°C overnight. The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma labeling Occludin with ab216327 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880), ready to use. Counter stained with Hematoxylin. Antigen retrieval was heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Secondary antibody only control: Rabbit specific IHC polymer detection kit HRP/DAB ab209101 (Rabbit specific IHC polymer detection kit HRP/DAB).
Positive staining on human thyroid carcinoma. The section was incubated with ab216327 at 4°C overnight.
Immunohistochemistry analysis of PFA-fixed, 0.3% Triton X-100 permeabilized frozen Human choroid plexus organoid (fresh frozen) tissue labelling Occludin with ab216327 at 1/200 dilution, followed by Donkey anti Rabbit (Alexa Fluor® 488) secondary antibody at 1/500 dilution dilution (green). The section was incubated with ab216327 for 24 hours at 4°C.
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