Rabbit Recombinant Monoclonal Occludin antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat, Dog, Recombinant full length protein - Human samples. Cited in 2 publications.
Images
![Western blot - Anti-Occludin antibody [EPR20992] - BSA and Azide free (AB224526), expandable thumbnail](https://content.abcam.com/products/images/occludin-antibody-epr20992-bsa-and-azide-free-ab224526--western-blot-img139434.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Occludin antibody [EPR20992] - BSA and Azide free (AB224526)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (AB224526), expandable thumbnail](https://content.abcam.com/products/images/occludin-antibody-epr20992-bsa-and-azide-free-ab224526--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img68696.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (AB224526)")
![Western blot - Anti-Occludin antibody [EPR20992] - BSA and Azide free (AB224526), expandable thumbnail](https://content.abcam.com/products/images/occludin-antibody-epr20992-bsa-and-azide-free-ab224526--western-blot-img165487.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Occludin antibody [EPR20992] - BSA and Azide free (AB224526)")
![Immunocytochemistry/ Immunofluorescence - Anti-Occludin antibody [EPR20992] - BSA and Azide free (AB224526), expandable thumbnail](https://content.abcam.com/products/images/occludin-antibody-epr20992-bsa-and-azide-free-ab224526--immunocytochemistry-immunofluorescence-img71841.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Occludin antibody [EPR20992] - BSA and Azide free (AB224526)")
![Western blot - Anti-Occludin antibody [EPR20992] - BSA and Azide free (AB224526), expandable thumbnail](https://content.abcam.com/products/images/occludin-antibody-epr20992-bsa-and-azide-free-ab224526--western-blot-img170764.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Occludin antibody [EPR20992] - BSA and Azide free (AB224526)")
Publications
Filter by
- Frontiers in pharmacology 12:7590272022QiShenYiQi Inhibits Tissue Plasminogen Activator-Induced Brain Edema and Hemorrhage after Ischemic Stroke in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Ye,Quan Li,Chun-Shui Pan,Li Yan,Kai Sun,Xiao-Yi Wang,Shu-Qi Yao,Jing-Yu Fan,Jing-Yan HanPubMed 35095486
- eNeuro 8:2021Beneficial Effects of Transplanted Human Bone Marrow Endothelial Progenitors on Functional and Cellular Components of Blood-Spinal Cord Barrier in ALS Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSvitlana Garbuzova-Davis,Kayla J Boccio,Alexander Llauget,Robert Shell,Surafuale Hailu,Hilmi Mustafa,Jared Ehrhart,Paul R Sanberg,Stanley H Appel,Cesario V BorlonganPubMed 34479980
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Expected | Tested | Not recommended | Expected | Tested |
| Rat | Expected | Tested | Not recommended | Expected | Tested |
| Dog | Expected | Tested | Tested | Expected | Expected |
| Recombinant full length protein - Human | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Dog | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Please upload more of the lysate or use lower dilution of Anti-Occludin antibody [EPR20992] ab216327 when testing bEnd.3. Occludin contains multiple isoforms, and the molecular weight range of different isoforms varies greatly (8kDa~59kDa). In addition, there are various post-translational modifications such as ubiquitination and proteolytic cleavage, so the band sizes and number of bands detected in different samples may vary. (PMID: 18647175, PMID: 19821483, PMID: 19457074, PMID: 11782481, PMID: 22083955). |
Species Rat | Dilution info - | Notes Please upload more of the lysate or use lower dilution of Anti-Occludin antibody [EPR20992] ab216327 when testing bEnd.3. Occludin contains multiple isoforms, and the molecular weight range of different isoforms varies greatly (8kDa~59kDa). In addition, there are various post-translational modifications such as ubiquitination and proteolytic cleavage, so the band sizes and number of bands detected in different samples may vary. (PMID: 18647175, PMID: 19821483, PMID: 19457074, PMID: 11782481, PMID: 22083955). |
Species Human | Dilution info - | Notes Please upload more of the lysate or use lower dilution of Anti-Occludin antibody [EPR20992] ab216327 when testing bEnd.3. Occludin contains multiple isoforms, and the molecular weight range of different isoforms varies greatly (8kDa~59kDa). In addition, there are various post-translational modifications such as ubiquitination and proteolytic cleavage, so the band sizes and number of bands detected in different samples may vary. (PMID: 18647175, PMID: 19821483, PMID: 19457074, PMID: 11782481, PMID: 22083955). |
Species Dog | Dilution info - | Notes Please upload more of the lysate or use lower dilution of Anti-Occludin antibody [EPR20992] ab216327 when testing bEnd.3. Occludin contains multiple isoforms, and the molecular weight range of different isoforms varies greatly (8kDa~59kDa). In addition, there are various post-translational modifications such as ubiquitination and proteolytic cleavage, so the band sizes and number of bands detected in different samples may vary. (PMID: 18647175, PMID: 19821483, PMID: 19457074, PMID: 11782481, PMID: 22083955). |
Species Recombinant full length protein - Human | Dilution info - | Notes Please upload more of the lysate or use lower dilution of Anti-Occludin antibody [EPR20992] ab216327 when testing bEnd.3. Occludin contains multiple isoforms, and the molecular weight range of different isoforms varies greatly (8kDa~59kDa). In addition, there are various post-translational modifications such as ubiquitination and proteolytic cleavage, so the band sizes and number of bands detected in different samples may vary. (PMID: 18647175, PMID: 19821483, PMID: 19457074, PMID: 11782481, PMID: 22083955). |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Dog | Dilution info - | Notes This antibody is not suitable for ICC in mouse or rat species. |
Species Human | Dilution info - | Notes This antibody is not suitable for ICC in mouse or rat species. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes This antibody is not suitable for ICC in mouse or rat species. |
Species Rat | Dilution info - | Notes This antibody is not suitable for ICC in mouse or rat species. |
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Dog | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Dog | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. (Microbial infection) Acts as a coreceptor for hepatitis C virus (HCV) in hepatocytes.
Alternative names
Occludin, OCLN
Recommended products
Rabbit Recombinant Monoclonal Occludin antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat, Dog, Recombinant full length protein - Human samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20992
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody is not suitable for ICC in mouse or rat species or for any testing rat intestinal tissues.
FURTHER INFORMATION ON SPECIFICITY (Chinese Version) available under the support & downloads section.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab224526 is the carrier-free version of Anti-Occludin antibody [EPR20992] ab216327.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Occludin is a protein that plays an important mechanical role in forming tight junctions which are specialized connections between neighboring cell membranes. Alternative names for this target include occludin-1. The occludin protein has a molecular weight of approximately 65 kDa. It is expressed in a variety of tissues but levels tend to be high in epithelial and endothelial cells where tight junctions are essential for barrier functions. Occludin acts as integral membrane protein contributing to the complex structures that seal spaces between cells.
- Biological function summary
Occludin serves as an important component in maintaining cell-to-cell adhesion within tight junction complexes. It interacts with other tight junction proteins such as claudins and junctional adhesion molecules to regulate paracellular permeability. These interactions help maintain selective barrier properties of epithelial and endothelial layers preventing the passage of large molecules and pathogens while allowing passage of ions and small molecules. Occludin stabilizes the tight junctions by interacting with the cytoskeleton and signaling proteins that are important for tight junction assembly and maintenance.
- Pathways
Occludin functions prominently in the regulation of tight junction integrity and barrier function. It participates in pathways such as the tight junction signaling pathway and the paracellular transport pathway. Occludin interacts with ZO-1 a scaffolding protein bridging its interaction with the actin cytoskeleton which supports the structural organization and function of tight junctions. This connectivity allows for regulation of the permeability and transport across the epithelial barrier.
- Associated diseases and disorders
Disruptions in occludin function are connected to conditions such as inflammatory bowel disease (IBD) and some cancers. In IBD abnormal occludin expression can compromise intestinal barrier function leading to increased intestinal permeability and an inflammatory response. In certain cancers loss of occludin expression is linked to increased invasiveness and metastasis due to weakened cell adhesion. The dysfunction of proteins like ZO-1 which interact with occludin in these pathways also contributes to these pathological states highlighting the importance of occludin's regulatory roles in maintaining cellular barrier integrity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
18 product images
![Western blot - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526), expandable thumbnail](https://content.abcam.com/products/images/occludin-antibody-epr20992-bsa-and-azide-free-ab224526--western-blot-img139434.jpg "Western blot - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)")
Western blot - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)
Lanes 1 - 4: Merged signal (red and green). Green - Anti-Occludin antibody [EPR20992] ab216327 observed at 59 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.
Anti-Occludin antibody [EPR20992] ab216327 was shown to recognize Occludin in wild-type HAP1 cells as signal was lost at the expected MW in OCLN (Occludin) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and OCLN (Occludin) knockout samples were subjected to SDS-PAGE. Anti-Occludin antibody [EPR20992] ab216327 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Occludin expression in HeLa is expected to be negative.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Occludin antibody [EPR20992] ab216327).
All lanes: Western blot - Anti-Occludin antibody [EPR20992] (Anti-Occludin antibody [EPR20992] ab216327) at 1/1000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 40 µg
Lane 2: OCLN (Occludin) knockout HAP1 whole cell lysate at 40 µg
Lane 3: HeLa whole cell lysate (Low Occludin expression) at 20 µg
Lane 4: HepG2 whole cell lysate lysate (High Occludin expression) at 20 µg
Predicted band size: 59 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526), expandable thumbnail](https://content.abcam.com/products/images/occludin-antibody-epr20992-bsa-and-azide-free-ab224526--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img68696.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Occludin with Anti-Occludin antibody [EPR20992] ab216327 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on human colon is observed (PMID: 24268521). Counter stained with Hematoxylin.
Secondary antibody only control: Rabbit specific IHC polymer detection kit HRP/DAB ab209101 (Rabbit specific IHC polymer detection kit HRP/DAB).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Occludin antibody [EPR20992] ab216327).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Western blot - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526), expandable thumbnail](https://content.abcam.com/products/images/occludin-antibody-epr20992-bsa-and-azide-free-ab224526--western-blot-img165487.jpg "Western blot - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)")
Western blot - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)
This data was developed using Anti-Occludin antibody [EPR20992] ab216327, the same antibody clone in a different buffer formulation.
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Occludin antibody [EPR20992] (Anti-Occludin antibody [EPR20992] ab216327) at 1/1000 dilution
Lane 1: Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: bEnd.3 (Mouse brain endothelioma) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution
Predicted band size: 59 kDa
Observed band size: 65 kDa
Exposure time: 60s
![Immunocytochemistry/ Immunofluorescence - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526), expandable thumbnail](https://content.abcam.com/products/images/occludin-antibody-epr20992-bsa-and-azide-free-ab224526--immunocytochemistry-immunofluorescence-img71841.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)")
Immunocytochemistry/ Immunofluorescence - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Caco-2 (human colorectal adenocarcinoma cell line) cells labeling Occludin with Anti-Occludin antibody [EPR20992] ab216327 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membrane staining on Caco-2 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Occludin antibody [EPR20992] ab216327).
![Western blot - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526), expandable thumbnail](https://content.abcam.com/products/images/occludin-antibody-epr20992-bsa-and-azide-free-ab224526--western-blot-img170764.jpg "Western blot - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)")
Western blot - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)
This data was developed using Anti-Occludin antibody [EPR20992] ab216327, the same antibody clone in a different buffer formulation.
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Occludin antibody [EPR20992] (Anti-Occludin antibody [EPR20992] ab216327) at 1/1000 dilution
Lane 1: Mouse colon tissue lysate at 20 µg
Lane 2: Mouse brain tissue lysate at 20 µg
Lane 3: Mouse lung tissue lysate at 20 µg
Lane 4: Rat colon tissue lysate at 20 µg
Lane 5: Rat brain tissue lysate at 20 µg
Lane 6: Rat lung tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution
Predicted band size: 59 kDa
Observed band size: 65 kDa
Exposure time: 100s
![Immunoprecipitation - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526), expandable thumbnail](https://content.abcam.com/products/images/occludin-antibody-epr20992-bsa-and-azide-free-ab224526--immunoprecipitation-img5037.jpg "Immunoprecipitation - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)")
Immunoprecipitation - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)
Occludin was immunoprecipitated from 0.35 mg of Caco-2 (human colorectal adenocarcinoma cell line) whole cell lysate with Anti-Occludin antibody [EPR20992] ab216327 at 1/40 dilution. Western blot was performed from the immunoprecipitate using Anti-Occludin antibody [EPR20992] ab216327 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: Caco-2 whole cell lysate 10 μg (Input).
Lane 2: Anti-Occludin antibody [EPR20992] ab216327 IP in Caco-2 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Occludin antibody [EPR20992] ab216327 in Caco-2 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
The molecular weight observed is consistent with what has been described in the literature (PMID: 18647175, PMID: 19821483).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Occludin antibody [EPR20992] ab216327).
All lanes: Immunoprecipitation - Anti-Occludin antibody [EPR20992] (Anti-Occludin antibody [EPR20992] ab216327)
Predicted band size: 59 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526), expandable thumbnail](https://content.abcam.com/products/images/occludin-antibody-epr20992-bsa-and-azide-free-ab224526--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img67007.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Occludin with Anti-Occludin antibody [EPR20992] ab216327 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on distal tubules of mouse kidney is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Rabbit specific IHC polymer detection kit HRP/DAB ab209101 (Rabbit specific IHC polymer detection kit HRP/DAB).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Occludin antibody [EPR20992] ab216327).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526), expandable thumbnail](https://content.abcam.com/products/images/occludin-antibody-epr20992-bsa-and-azide-free-ab224526--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131030.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Occludin with Anti-Occludin antibody [EPR20992] ab216327 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on distal tubules of rat kidney is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Rabbit specific IHC polymer detection kit HRP/DAB ab209101 (Rabbit specific IHC polymer detection kit HRP/DAB).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Occludin antibody [EPR20992] ab216327).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526), expandable thumbnail](https://content.abcam.com/products/images/occludin-antibody-epr20992-bsa-and-azide-free-ab224526--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132763.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Occludin antibody [EPR20992] ab216327).
Immunohistochemical analysis of paraffin-embedded human breast tissue labeling Occludin with Anti-Occludin antibody [EPR20992] ab216327 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on human breast is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Rabbit specific IHC polymer detection kit HRP/DAB ab209101 (Rabbit specific IHC polymer detection kit HRP/DAB).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526), expandable thumbnail](https://content.abcam.com/products/images/occludin-antibody-epr20992-bsa-and-azide-free-ab224526--immunocytochemistry-immunofluorescence-img22922.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)")
Immunocytochemistry/ Immunofluorescence - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDCK (canine kidney cell line) cells labeling Occludin with Anti-Occludin antibody [EPR20992] ab216327 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membrane staining on MDCK (NBL-2) cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Occludin antibody [EPR20992] ab216327).
![Flow Cytometry (Intracellular) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526), expandable thumbnail](https://content.abcam.com/products/images/occludin-antibody-epr20992-bsa-and-azide-free-ab224526--flow-cytometry-intracellular-img26076.jpg "Flow Cytometry (Intracellular) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)")
Flow Cytometry (Intracellular) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Caco-2 (human colorectal adenocarcinoma cell line) cell line labeling Occludin with Anti-Occludin antibody [EPR20992] ab216327 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Occludin antibody [EPR20992] ab216327).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526), expandable thumbnail](https://content.abcam.com/products/images/occludin-antibody-epr20992-bsa-and-azide-free-ab224526--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img438036.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)
Immunohistochemical analysis of paraffin-embedded Rat colon labeling Occludin with Anti-Occludin antibody [EPR20992] ab216327 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Positive staining on Rat colon. The section was incubated with Anti-Occludin antibody [EPR20992] ab216327 at 4°C overnight. The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This data was developed using Anti-Occludin antibody [EPR20992] ab216327, the same antibody clone in a different buffer formulation.
![Western blot - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526), expandable thumbnail](https://content.abcam.com/products/images/occludin-antibody-epr20992-bsa-and-azide-free-ab224526--western-blot-img438232.jpg "Western blot - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)")
Western blot - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Occludin antibody [EPR20992] ab216327).
For cells express relatively low level of Occludin, we suggest optimizing experimental protocols (increasing lysate amount, using lower dilution or higher sensitivity ECL substrate) to improve results.
Blocking and Diluting buffer and concentration: 5% NFDM /TBST
Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a GAPDH loading control.
All lanes: Western blot - Anti-Occludin antibody [EPR20992] (Anti-Occludin antibody [EPR20992] ab216327) at 1/1000 dilution
Lane 1: Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: DLD-1(human colorectal adenocarcinoma cell) whole cell lysate at 20 µg
Lane 3: A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 5: HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 16-60 kDa
Exposure time: 20s
![Western blot - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526), expandable thumbnail](https://content.abcam.com/products/images/occludin-antibody-epr20992-bsa-and-azide-free-ab224526--western-blot-img438233.jpg "Western blot - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)")
Western blot - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Occludin antibody [EPR20992] ab216327).
Occludin contains multiple isoforms, and the molecular weight range of different isoforms varies greatly (8kDa~59kDa). In addition, there are various post-translational modifications such as ubiquitination and proteolytic cleavage, so the band sizes and number of bands detected in different samples may vary. (PMID: 18647175, PMID: 19821483, PMID: 19457074, PMID: 11782481, PMID: 22083955)
Blocking and Diluting buffer and concentration: 5% NFDM /TBST
Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a GAPDH loading control.
All lanes: Western blot - Anti-Occludin antibody [EPR20992] (Anti-Occludin antibody [EPR20992] ab216327) at 1/1000 dilution
Lane 1: Mouse colon tissue lysate at 20 µg
Lane 2: Mouse small intestine tissue lysate at 20 µg
Lane 3: Mouse ileum tissue lysate at 20 µg
Lane 4: Mouse liver tissue lysate at 20 µg
Lane 5: Mouse Kidney tissue lysate at 20 µg
Lane 6: Human colon tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 16-60 kDa
Exposure time: 100s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526), expandable thumbnail](https://content.abcam.com/products/images/occludin-antibody-epr20992-bsa-and-azide-free-ab224526--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img417483.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Occludin antibody [EPR20992] ab216327).
Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma labeling Occludin with Anti-Occludin antibody [EPR20992] ab216327 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880), ready to use. Counter stained with Hematoxylin. Antigen retrieval was heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Secondary antibody only control: Rabbit specific IHC polymer detection kit HRP/DAB ab209101 (Rabbit specific IHC polymer detection kit HRP/DAB).
Positive staining on human thyroid carcinoma. The section was incubated with Anti-Occludin antibody [EPR20992] ab216327 at 4°C overnight.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526), expandable thumbnail](https://content.abcam.com/products/images/occludin-antibody-epr20992-bsa-and-azide-free-ab224526--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img438038.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)
Immunohistochemical analysis of paraffin-embedded Human colon labeling Occludin with Anti-Occludin antibody [EPR20992] ab216327 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Positive staining on human colon. The section was incubated with Anti-Occludin antibody [EPR20992] ab216327 at 4°C overnight. The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This data was developed using Anti-Occludin antibody [EPR20992] ab216327, the same antibody clone in a different buffer formulation.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526), expandable thumbnail](https://content.abcam.com/products/images/occludin-antibody-epr20992-bsa-and-azide-free-ab224526--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img417797.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Occludin antibody [EPR20992] ab216327).
Tissue Microarrays stained for Anti-Occludin antibody [EPR20992] using Anti-Occludin antibody [EPR20992] ab216327 in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negaive (cross mark) staining per sample type tested. The sections were incubated with Anti-Occludin antibody [EPR20992] ab216327 at 4°C overnight used at 1:2000 dilution (1.05 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Counterstain was Hematoxylin.
Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526), expandable thumbnail](https://content.abcam.com/products/images/occludin-antibody-epr20992-bsa-and-azide-free-ab224526--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img438037.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)
Immunohistochemical analysis of paraffin-embedded Mouse colon labeling Occludin with Anti-Occludin antibody [EPR20992] ab216327 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Positive staining on Mouse colon. The section was incubated with Anti-Occludin antibody [EPR20992] ab216327 at 4°C overnight. The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This data was developed using Anti-Occludin antibody [EPR20992] ab216327, the same antibody clone in a different buffer formulation.
![Immunoprecipitation - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526), expandable thumbnail](https://content.abcam.com/products/images/occludin-antibody-epr20992-bsa-and-azide-free-ab224526--immunoprecipitation-img5037.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526), expandable thumbnail](https://content.abcam.com/products/images/occludin-antibody-epr20992-bsa-and-azide-free-ab224526--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img67007.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526), expandable thumbnail](https://content.abcam.com/products/images/occludin-antibody-epr20992-bsa-and-azide-free-ab224526--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131030.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526), expandable thumbnail](https://content.abcam.com/products/images/occludin-antibody-epr20992-bsa-and-azide-free-ab224526--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132763.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)")
![Immunocytochemistry/ Immunofluorescence - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526), expandable thumbnail](https://content.abcam.com/products/images/occludin-antibody-epr20992-bsa-and-azide-free-ab224526--immunocytochemistry-immunofluorescence-img22922.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)")
![Flow Cytometry (Intracellular) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526), expandable thumbnail](https://content.abcam.com/products/images/occludin-antibody-epr20992-bsa-and-azide-free-ab224526--flow-cytometry-intracellular-img26076.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526), expandable thumbnail](https://content.abcam.com/products/images/occludin-antibody-epr20992-bsa-and-azide-free-ab224526--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img438036.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)")
![Western blot - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526), expandable thumbnail](https://content.abcam.com/products/images/occludin-antibody-epr20992-bsa-and-azide-free-ab224526--western-blot-img438232.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)")
![Western blot - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526), expandable thumbnail](https://content.abcam.com/products/images/occludin-antibody-epr20992-bsa-and-azide-free-ab224526--western-blot-img438233.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526), expandable thumbnail](https://content.abcam.com/products/images/occludin-antibody-epr20992-bsa-and-azide-free-ab224526--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img417483.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526), expandable thumbnail](https://content.abcam.com/products/images/occludin-antibody-epr20992-bsa-and-azide-free-ab224526--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img438038.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526), expandable thumbnail](https://content.abcam.com/products/images/occludin-antibody-epr20992-bsa-and-azide-free-ab224526--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img417797.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526), expandable thumbnail](https://content.abcam.com/products/images/occludin-antibody-epr20992-bsa-and-azide-free-ab224526--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img438037.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)")
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