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AB125917

Anti-OCRL antibody

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(1 Publication)

Rabbit Polyclonal OCRL antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human OCRL aa 550-900.

View Alternative Names

OCRL1, OCRL, Inositol polyphosphate 5-phosphatase OCRL, Inositol polyphosphate 5-phosphatase OCRL-1, Lowe oculocerebrorenal syndrome protein, OCRL-1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-OCRL antibody (AB125917)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-OCRL antibody (AB125917)

ab125917, at 1/500, staining OCRL in Human A431 cells (methanol-fixed) by immunofluorescence (panel 1) and co-stained with Hoechst 33342 (panel 2).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OCRL antibody (AB125917)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OCRL antibody (AB125917)

ab125917, at 1/250, staining OCRL in Human Breast ca tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].

Western blot - Anti-OCRL antibody (AB125917)
  • WB

Unknown

Western blot - Anti-OCRL antibody (AB125917)

Gel concentration : 5%

All lanes:

Western blot - Anti-OCRL antibody (ab125917) at 1/500 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Predicted band size: 104 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human OCRL aa 550-900. The exact immunogen used to generate this antibody is proprietary information.

Q01968

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>citrate buffer-10mM</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The OCRL protein also known as oculocerebrorenal syndrome of Lowe protein is a phosphatidylinositol 45-bisphosphate 5-phosphatase involved in cellular membrane trafficking. It has a molecular mass of approximately 105 kDa. Localization of OCRL occurs in several cellular compartments including the endosomes and the Golgi apparatus. This protein is widely expressed in tissues such as the brain kidney and eyes. Scientists often study OCRL to better understand its role in cellular functions and associated disorders.
Biological function summary

OCRL participates in the regulation of phosphoinositide metabolism by dephosphorylating phosphatidylinositol 45-bisphosphate an important signaling lipid. OCRL is a member of the inositol polyphosphate 5-phosphatase family and associates with clathrin-mediated endocytosis complexes. The protein ensures proper vesicular trafficking and maintenance of cell membrane dynamics impacting processes like endocytosis and ciliogenesis. Researchers focus on understanding OCRL's interactions and dynamics to comprehend its contribution to these processes.

Pathways

OCRL plays a significant role in the phosphoinositide signaling pathway and is integral to cellular trafficking pathways. In the phosphoinositide signaling pathway OCRL modulates the availability of phosphatidylinositol 45-bisphosphate interacting with proteins like APPL1 and Rab GTPases. In cellular trafficking pathways OCRL coordinates with proteins such as clathrin and dynamin to regulate endocytosis and endosomal function. Understanding OCRL's placement within these pathways provides insight into its impact on cellular homeostasis.

OCRL mutations are linked to Lowe syndrome and Dent disease both of which involve renal and ocular complications. Lowe syndrome characterized by congenital cataracts intellectual disabilities and renal Fanconi syndrome directly associates with OCRL dysfunction. The OCRL protein interacts with clathrin and Rab GTPases contributing to the pathogenesis of these disorders by disrupting normal vesicle trafficking processes. Research on OCRL-related mechanisms in these diseases seeks to unravel potential therapeutic targets and interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the hydrolysis of the 5-position phosphate of phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and phosphatidylinositol-3,4,5-bisphosphate (PtdIns(3,4,5)P3), with the greatest catalytic activity towards PtdIns(4,5)P2 (PubMed : 10764818, PubMed : 15474001, PubMed : 7761412, PubMed : 9430698). Able also to hydrolyze the 5-phosphate of inositol 1,4,5-trisphosphate and of inositol 1,3,4,5-tetrakisphosphate (PubMed : 25869668, PubMed : 7761412). Regulates traffic in the endosomal pathway by regulating the specific pool of phosphatidylinositol 4,5-bisphosphate that is associated with endosomes (PubMed : 21971085). Involved in primary cilia assembly (PubMed : 22228094, PubMed : 22543976). Acts as a regulator of phagocytosis, hydrolyzing PtdIns(4,5)P2 to promote phagosome closure, through attenuation of PI3K signaling (PubMed : 22072788).
See full target information OCRL
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 12:3481 PubMed34108482

2021

TcpC inhibits neutrophil extracellular trap formation by enhancing ubiquitination mediated degradation of peptidylarginine deiminase 4.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Ou,Jia-Qi Fang,Zhe-Sheng Zhang,Zhe Chi,Jie Fang,Di-Yan Xu,Kai-Zhong Lu,Meng-Qing Qian,Da-Yong Zhang,Jun-Ping Guo,Wei Gao,Na-Ru Zhang,Jian-Ping Pan
View all publications
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Product promise

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