Anti-OCRL antibody [EP10256]

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-OCRL antibody [EP10256] (AB181039)

Intracellular flow cytometric analysis of permeabilized SH-SY5Y cells using ab181039 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

• WB

Lab

Western blot - Anti-OCRL antibody [EP10256] (AB181039)

Western blot : Anti-OCRL antibody [EP10256] ab181039 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 104 kDa in Wild-type U-87 MG cell lysates with no signal observed at this size in OCRL knockout U-87 MG cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-OCRL antibody [EP10256] (ab181039) at 1/1000 dilution

Lane 1:

Wild-type U-87 MG cell lysates at 20 µg

Lane 2:

OCRL knockout U-87 MG cell lysates at 20 µg

Lane 3:

PC-3 cell lysates at 20 µg

Lane 4:

THP-1 cell lysates at 20 µg

Lane 5:

Raji cell lysates at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 104 kDa

Observed band size: 104 kDa

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• WB

Supplier Data

Western blot - Anti-OCRL antibody [EP10256] (AB181039)

All lanes:

Western blot - Anti-OCRL antibody [EP10256] (ab181039) at 1000 µg

Lane 1:

293T cell lysates at 10 µg

Lane 2:

SH-SY5Y cell lysates at 10 µg

Lane 3:

HeLa cell lysates at 10 µg

Lane 4:

JAR cell lysates at 10 µg

Predicted band size: 104 kDa

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