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Rabbit Monoclonal OCRL antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

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Images

Western blot - Anti-OCRL antibody [EP10256] - BSA and Azide free (AB284833), expandable thumbnail
  • Western blot - Anti-OCRL antibody [EP10256] - BSA and Azide free (AB284833), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-OCRL antibody [EP10256] - BSA and Azide free (AB284833), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IFFlow Cyt (Intra)
Human
Not recommended
Tested
Not recommended
Not recommended
Tested

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target data

Function

Catalyzes the hydrolysis of the 5-position phosphate of phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and phosphatidylinositol-3,4,5-bisphosphate (PtdIns(3,4,5)P3), with the greatest catalytic activity towards PtdIns(4,5)P2 (PubMed:10764818, PubMed:15474001, PubMed:7761412, PubMed:9430698). Able also to hydrolyze the 5-phosphate of inositol 1,4,5-trisphosphate and of inositol 1,3,4,5-tetrakisphosphate (PubMed:25869668, PubMed:7761412). Regulates traffic in the endosomal pathway by regulating the specific pool of phosphatidylinositol 4,5-bisphosphate that is associated with endosomes (PubMed:21971085). Involved in primary cilia assembly (PubMed:22228094, PubMed:22543976). Acts as a regulator of phagocytosis, hydrolyzing PtdIns(4,5)P2 to promote phagosome closure, through attenuation of PI3K signaling (PubMed:22072788).

Alternative names

OCRL1, OCRL, Inositol polyphosphate 5-phosphatase OCRL, Inositol polyphosphate 5-phosphatase OCRL-1, Lowe oculocerebrorenal syndrome protein, OCRL-1

Recommended products

Rabbit Monoclonal OCRL antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EP10256
Purity
Tissue culture supernatant
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab284833 is the carrier-free version of Anti-OCRL antibody [EP10256] ab181039

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The OCRL protein also known as oculocerebrorenal syndrome of Lowe protein is a phosphatidylinositol 45-bisphosphate 5-phosphatase involved in cellular membrane trafficking. It has a molecular mass of approximately 105 kDa. Localization of OCRL occurs in several cellular compartments including the endosomes and the Golgi apparatus. This protein is widely expressed in tissues such as the brain kidney and eyes. Scientists often study OCRL to better understand its role in cellular functions and associated disorders.

Biological function summary

OCRL participates in the regulation of phosphoinositide metabolism by dephosphorylating phosphatidylinositol 45-bisphosphate an important signaling lipid. OCRL is a member of the inositol polyphosphate 5-phosphatase family and associates with clathrin-mediated endocytosis complexes. The protein ensures proper vesicular trafficking and maintenance of cell membrane dynamics impacting processes like endocytosis and ciliogenesis. Researchers focus on understanding OCRL's interactions and dynamics to comprehend its contribution to these processes.

Pathways

OCRL plays a significant role in the phosphoinositide signaling pathway and is integral to cellular trafficking pathways. In the phosphoinositide signaling pathway OCRL modulates the availability of phosphatidylinositol 45-bisphosphate interacting with proteins like APPL1 and Rab GTPases. In cellular trafficking pathways OCRL coordinates with proteins such as clathrin and dynamin to regulate endocytosis and endosomal function. Understanding OCRL's placement within these pathways provides insight into its impact on cellular homeostasis.

Associated diseases and disorders

OCRL mutations are linked to Lowe syndrome and Dent disease both of which involve renal and ocular complications. Lowe syndrome characterized by congenital cataracts intellectual disabilities and renal Fanconi syndrome directly associates with OCRL dysfunction. The OCRL protein interacts with clathrin and Rab GTPases contributing to the pathogenesis of these disorders by disrupting normal vesicle trafficking processes. Research on OCRL-related mechanisms in these diseases seeks to unravel potential therapeutic targets and interventions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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