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AB284833

Anti-OCRL antibody [EP10256] - BSA and Azide free

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Rabbit Monoclonal OCRL antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

OCRL1, OCRL, Inositol polyphosphate 5-phosphatase OCRL, Inositol polyphosphate 5-phosphatase OCRL-1, Lowe oculocerebrorenal syndrome protein, OCRL-1

3 Images
Flow Cytometry (Intracellular) - Anti-OCRL antibody [EP10256] - BSA and Azide free (AB284833)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-OCRL antibody [EP10256] - BSA and Azide free (AB284833)

This data was developed using ab181039, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized SH-SY5Y cells using ab181039 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

Western blot - Anti-OCRL antibody [EP10256] - BSA and Azide free (AB284833)
  • WB

Supplier Data

Western blot - Anti-OCRL antibody [EP10256] - BSA and Azide free (AB284833)

This data was developed using ab181039, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-OCRL antibody [EP10256] (<a href='/en-us/products/primary-antibodies/ocrl-antibody-ep10256-ab181039'>ab181039</a>) at 1000 µg

Lane 1:

293T cell lysates at 10 µg

Lane 2:

SH-SY5Y cell lysates at 10 µg

Lane 3:

HeLa cell lysates at 10 µg

Lane 4:

JAR cell lysates at 10 µg

Predicted band size: 104 kDa

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Western blot - Anti-OCRL antibody [EP10256] - BSA and Azide free (AB284833)
  • WB

Lab

Western blot - Anti-OCRL antibody [EP10256] - BSA and Azide free (AB284833)

This data was developed using ab181039, the same antibody clone in a different buffer formulation.

Lane 1 : Wild-type HAP1 cell lysate (20 μg)

Lane 2 : INPP5F knockout HAP1 cell lysate (20 μg)

Lane 3 : SH-SY5Y cell lysate (20 μg)

Lane 4 : HeLa cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab181039 observed at 105 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab181039 was shown to recognize INPP5F when INPP5F knockout samples were used, along with additional cross-reactive bands. Wild-type and INPP5F knockout samples were subjected to SDS-PAGE. ab181039 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-OCRL antibody [EP10256] (<a href='/en-us/products/primary-antibodies/ocrl-antibody-ep10256-ab181039'>ab181039</a>)

Predicted band size: 104 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP10256

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab284833 is the carrier-free version of ab181039

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The OCRL protein also known as oculocerebrorenal syndrome of Lowe protein is a phosphatidylinositol 45-bisphosphate 5-phosphatase involved in cellular membrane trafficking. It has a molecular mass of approximately 105 kDa. Localization of OCRL occurs in several cellular compartments including the endosomes and the Golgi apparatus. This protein is widely expressed in tissues such as the brain kidney and eyes. Scientists often study OCRL to better understand its role in cellular functions and associated disorders.
Biological function summary

OCRL participates in the regulation of phosphoinositide metabolism by dephosphorylating phosphatidylinositol 45-bisphosphate an important signaling lipid. OCRL is a member of the inositol polyphosphate 5-phosphatase family and associates with clathrin-mediated endocytosis complexes. The protein ensures proper vesicular trafficking and maintenance of cell membrane dynamics impacting processes like endocytosis and ciliogenesis. Researchers focus on understanding OCRL's interactions and dynamics to comprehend its contribution to these processes.

Pathways

OCRL plays a significant role in the phosphoinositide signaling pathway and is integral to cellular trafficking pathways. In the phosphoinositide signaling pathway OCRL modulates the availability of phosphatidylinositol 45-bisphosphate interacting with proteins like APPL1 and Rab GTPases. In cellular trafficking pathways OCRL coordinates with proteins such as clathrin and dynamin to regulate endocytosis and endosomal function. Understanding OCRL's placement within these pathways provides insight into its impact on cellular homeostasis.

OCRL mutations are linked to Lowe syndrome and Dent disease both of which involve renal and ocular complications. Lowe syndrome characterized by congenital cataracts intellectual disabilities and renal Fanconi syndrome directly associates with OCRL dysfunction. The OCRL protein interacts with clathrin and Rab GTPases contributing to the pathogenesis of these disorders by disrupting normal vesicle trafficking processes. Research on OCRL-related mechanisms in these diseases seeks to unravel potential therapeutic targets and interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the hydrolysis of the 5-position phosphate of phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and phosphatidylinositol-3,4,5-bisphosphate (PtdIns(3,4,5)P3), with the greatest catalytic activity towards PtdIns(4,5)P2 (PubMed : 10764818, PubMed : 15474001, PubMed : 7761412, PubMed : 9430698). Able also to hydrolyze the 5-phosphate of inositol 1,4,5-trisphosphate and of inositol 1,3,4,5-tetrakisphosphate (PubMed : 25869668, PubMed : 7761412). Regulates traffic in the endosomal pathway by regulating the specific pool of phosphatidylinositol 4,5-bisphosphate that is associated with endosomes (PubMed : 21971085). Involved in primary cilia assembly (PubMed : 22228094, PubMed : 22543976). Acts as a regulator of phagocytosis, hydrolyzing PtdIns(4,5)P2 to promote phagosome closure, through attenuation of PI3K signaling (PubMed : 22072788).
See full target information OCRL
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Product promise

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