AB272867

Anti-Oct-1 antibody

Name: Anti-Oct-1 antibody (ab272867) | Abcam
Brand: Abcam Limited
SKU: AB272867

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(1 Publication)

Rabbit Polyclonal Oct-1 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human POU2F1.

View Alternative Names

OCT1, OTF1, POU2F1, NF-A1, Octamer-binding protein 1, Octamer-binding transcription factor 1, Oct-1, OTF-1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-1 antibody (AB272867)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-1 antibody (AB272867)

Paraffin-embedded HeLa xenograft tissue stained for Oct-1 using ab272867 at 1/750 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.

Immunocytochemistry/ Immunofluorescence - Anti-Oct-1 antibody (AB272867)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Oct-1 antibody (AB272867)

4% PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Oct-1 using ab272867 at 1/500 dilution in ICC/IF.

Red : Phalloidin, a cytoskeleton marker.

Blue : Hoechst 33342 staining.

Scale bar = 10 μm.

Unknown

Immunoprecipitation - Anti-Oct-1 antibody (AB272867)

Samples : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate :

A : Control with 2.5 μg of pre-immune rabbit IgG.

B : Immunoprecipitation of Oct-1 by 2.5 μg of ab272867.

7.5% SDS-PAGE

The immunoprecipitated Oct-1 protein was detected by ab272867 at 1 : 1000 dilution, followed by anti-rabbit IgG (HRP).

All lanes:

Immunoprecipitation - Anti-Oct-1 antibody (ab272867)

Predicted band size: 76 kDa

false

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-1 antibody (AB272867)

Paraffin-embedded rat brain tissue stained for Oct-1 protein using ab272867 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

ICC/IF, WB, IHC-P, IP

applications

Immunogen

Recombinant Fragment Protein within Human POU2F1. The exact immunogen used to generate this antibody is proprietary information.

P14859

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Oct-1 also known as POU domain class 2 transcription factor 1 (POU2F1) is a transcription factor characterized by its POU-specific domain. The molecular weight of Oct-1 is approximately 76 kDa. It is expressed widely across different tissues with high levels found in lymphoid organs and epithelial cells. It binds to octamer motifs in the promoters of target genes regulating their transcription. Oct-1 plays a role in the cell cycle development and immune response.

Biological function summary

Oct-1 participates in the regulation of gene expression by influencing RNA polymerase II. It is not part of a larger protein complex for its primary function but it does interact with other cofactors. Oct-1 is essential for maintaining the expression of housekeeping genes as well as for the induction of specific genes during external stimuli responses. By modulating cellular responses Oct-1 helps in the regulation of cells' growth differentiation and adaptation to stress.

Pathways

Oct-1 is involved in pathways related to stress responses and immune system function. It significantly influences the transcriptional activation of genes associated with the stress response pathway. Oct-1 associates with proteins like c-Myc and others within these pathways which contribute to the regulation of cell proliferation and apoptosis. Its role in these pathways highlights its involvement in cellular homeostasis and survival.

Oct-1's dysregulation connects to the pathogenesis of various conditions including cancer and autoimmune diseases. Changes in Oct-1 activity have been observed in several cancers affecting tumorigenesis and progression. It interacts with proteins like Bcl-2 in the context of cancer impacting cell survival mechanisms. In autoimmune diseases such as lupus altered Oct-1 function can lead to inappropriate immune responses partly through interaction with transcription factors like NF-κB. Understanding the role of Oct-1 can help in developing targeted therapeutic strategies for these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR.. (Microbial infection) In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.

See full target information POU2F1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e37254 PubMed39309830

2024

Knockdown of LncRNA-HAGLR restrains the viability and motility of pancreatic cancer via miR-625-5p/TAF15 axis and .

Applications

Unspecified application

Species

Unspecified reactive species

Jiafu Wang,Huiqi Gao,Peng Fu,Lin Lin,Lifan Wang,Yue Han

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Oct-1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-1 antibody (AB272867)

Immunocytochemistry/ Immunofluorescence - Anti-Oct-1 antibody (AB272867)

Immunoprecipitation - Anti-Oct-1 antibody (AB272867)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-1 antibody (AB272867)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise