Anti-Oct-1 antibody [EPR16570]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-1 antibody [EPR16570] (AB178869)

Immunohistochemical analysis of paraffin-embedded Human endometrial cancer tissue labeling Oct-1 with ab178869 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on tumor cells of Human endometrial cancer tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-1 antibody [EPR16570] (AB178869)

Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling Oct-1 with ab178869 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on Human endometrial tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-1 antibody [EPR16570] (AB178869)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Oct-1 with ab178869 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on Human tonsil tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-1 antibody [EPR16570] (AB178869)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Oct-1 with ab178869 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on epithelium of mouse colon is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-1 antibody [EPR16570] (AB178869)

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Oct-1 with ab178869 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on rat spleen tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-Oct-1 antibody [EPR16570] (AB178869)

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 9398664; 14586402; 11777930).

All lanes:

Western blot - Anti-Oct-1 antibody [EPR16570] (ab178869) at 1/5000 dilution

Lane 1:

Ramos (Human Burkitt's lymphoma cell line) whole cell lysate at 10 µg

Lane 2:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 10 µg

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 76 kDa

Observed band size: 95 kDa

false

Exposure time: 3s

• WB

Supplier Data

Western blot - Anti-Oct-1 antibody [EPR16570] (AB178869)

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 9398664; 14586402; 11777930).

All lanes:

Western blot - Anti-Oct-1 antibody [EPR16570] (ab178869) at 1/1000 dilution

All lanes:

Human fetal brain lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 134 kDa,150 kDa,23 kDa,27 kDa,35 kDa,42 kDa,44 kDa,48 kDa,51 kDa,69 kDa,76 kDa,86 kDa,87 kDa,92 kDa

Observed band size: 100 kDa,110 kDa,130 kDa,160 kDa,180 kDa,23 kDa,27 kDa,28 kDa,35 kDa,42 kDa,45 kDa,51 kDa,85 kDa,95 kDa

false

Exposure time: 1min

• WB

Supplier Data

Western blot - Anti-Oct-1 antibody [EPR16570] (AB178869)

Oct-1 was immunoprecipitated from 0.35mg HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate with ab178869 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab178869 at 1/1000 dilution (0.357 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution. Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate (10ug). Lane 2 : ab178869 IP in HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab178869 in HeLa whole cell lysate. Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 24 seconds. Observed band : 76 kDa. Lysates were made freshly and used in IP test immediately to minimize protein degradation.

All lanes:

Western blot - Anti-Oct-1 antibody [EPR16570] (ab178869) at 1/30 dilution

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Predicted band size: 76 kDa

Observed band size: 76 kDa

false

Exposure time: 24s

• WB

Lab

Western blot - Anti-Oct-1 antibody [EPR16570] (AB178869)

Exposure time : Lane 1-3 : 15 seconds, Lane 4 : 60 seconds

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Please note that the antibody detects extra bands on lysates from mouse and rat cell lines

All lanes:

Western blot - Anti-Oct-1 antibody [EPR16570] (ab178869) at 1/1000 dilution

Lane 1:

C6 (rat glioma) whole cell lysate at 10 µg

Lane 2:

Raw 264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate at 10 µg

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Lane 4:

NIH/3T3 (mouse embryo) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 76 kDa

Observed band size: 95 kDa

false

• WB

Supplier Data

Western blot - Anti-Oct-1 antibody [EPR16570] (AB178869)

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 9398664; 14586402; 11777930).

Please note that the antibody detects extra bands on lysates from mouse and rat cell lines.

All lanes:

Western blot - Anti-Oct-1 antibody [EPR16570] (ab178869) at 1/1000 dilution

Lane 1:

Mouse heart lysate at 10 µg

Lane 2:

Rat heart lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 76 kDa

Observed band size: 95 kDa

false

Exposure time: 1min