Name: Anti-Oct-1 antibody [EPR16570]
SKU: ab178869
Rating: 5 (6 reviews)

Rabbit Recombinant Monoclonal Oct-1 antibody. Suitable for WB, IHC-P, IP and reacts with Mouse, Rat, Human samples. Cited in 11 publications.

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-1 antibody [EPR16570] (AB178869), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	IP
Human	Tested	Tested	Tested
Mouse	Tested	Tested	Not recommended
Rat	Tested	Tested	Expected

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Associated Products

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Target data

See full target information POU2F1

Function

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR. (Microbial infection) In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.

Alternative names

OCT1, OTF1, POU2F1, NF-A1, Octamer-binding protein 1, Octamer-binding transcription factor 1, Oct-1, OTF-1

Recommended products

Rabbit Recombinant Monoclonal Oct-1 antibody. Suitable for WB, IHC-P, IP and reacts with Mouse, Rat, Human samples. Cited in 11 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR16570
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Oct-1 also known as POU domain class 2 transcription factor 1 (POU2F1) is a transcription factor characterized by its POU-specific domain. The molecular weight of Oct-1 is approximately 76 kDa. It is expressed widely across different tissues with high levels found in lymphoid organs and epithelial cells. It binds to octamer motifs in the promoters of target genes regulating their transcription. Oct-1 plays a role in the cell cycle development and immune response.

Biological function summary

Oct-1 participates in the regulation of gene expression by influencing RNA polymerase II. It is not part of a larger protein complex for its primary function but it does interact with other cofactors. Oct-1 is essential for maintaining the expression of housekeeping genes as well as for the induction of specific genes during external stimuli responses. By modulating cellular responses Oct-1 helps in the regulation of cells' growth differentiation and adaptation to stress.

Pathways

Oct-1 is involved in pathways related to stress responses and immune system function. It significantly influences the transcriptional activation of genes associated with the stress response pathway. Oct-1 associates with proteins like c-Myc and others within these pathways which contribute to the regulation of cell proliferation and apoptosis. Its role in these pathways highlights its involvement in cellular homeostasis and survival.

Associated diseases and disorders

Oct-1's dysregulation connects to the pathogenesis of various conditions including cancer and autoimmune diseases. Changes in Oct-1 activity have been observed in several cancers affecting tumorigenesis and progression. It interacts with proteins like Bcl-2 in the context of cancer impacting cell survival mechanisms. In autoimmune diseases such as lupus altered Oct-1 function can lead to inappropriate immune responses partly through interaction with transcription factors like NF-κB. Understanding the role of Oct-1 can help in developing targeted therapeutic strategies for these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-1 antibody [EPR16570] (ab178869)
Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling Oct-1 with ab178869 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nucleus staining on Human endometrial tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-1 antibody [EPR16570] (ab178869)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Oct-1 with ab178869 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nucleus staining on Human tonsil tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-1 antibody [EPR16570] (ab178869)
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Oct-1 with ab178869 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nucleus staining on epithelium of mouse colon is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-1 antibody [EPR16570] (ab178869)
Immunohistochemical analysis of paraffin-embedded Human endometrial cancer tissue labeling Oct-1 with ab178869 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nucleus staining on tumor cells of Human endometrial cancer tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-1 antibody [EPR16570] (ab178869)
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Oct-1 with ab178869 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nucleus staining on rat spleen tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Oct-1 antibody [EPR16570] (ab178869)
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 9398664; 14586402; 11777930).
All lanes: Western blot - Anti-Oct-1 antibody [EPR16570] (ab178869) at 1/1000 dilution
All lanes: Human fetal brain lysate at 10 µg
Secondary
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 134 kDa, 150 kDa, 23 kDa, 27 kDa, 35 kDa, 42 kDa, 44 kDa, 48 kDa, 51 kDa, 69 kDa, 76 kDa, 86 kDa, 87 kDa, 92 kDa
Observed band size: 100 kDa, 110 kDa, 130 kDa, 160 kDa, 180 kDa, 23 kDa, 27 kDa, 28 kDa, 35 kDa, 42 kDa, 45 kDa, 51 kDa, 85 kDa, 95 kDa
Exposure time: 1min
Western blot - Anti-Oct-1 antibody [EPR16570] (ab178869)
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 9398664; 14586402; 11777930).
All lanes: Western blot - Anti-Oct-1 antibody [EPR16570] (ab178869) at 1/5000 dilution
Lane 1: Ramos (Human Burkitt's lymphoma cell line) whole cell lysate at 10 µg
Lane 2: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 10 µg
Lane 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 76 kDa
Observed band size: 95 kDa
Exposure time: 3s
Western blot - Anti-Oct-1 antibody [EPR16570] (ab178869)
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 9398664; 14586402; 11777930).
Please note that the antibody detects extra bands on lysates from mouse and rat cell lines.
All lanes: Western blot - Anti-Oct-1 antibody [EPR16570] (ab178869) at 1/1000 dilution
Lane 1: Mouse heart lysate at 10 µg
Lane 2: Rat heart lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 76 kDa
Observed band size: 95 kDa
Exposure time: 1min
Western blot - Anti-Oct-1 antibody [EPR16570] (ab178869)
Oct-1 was immunoprecipitated from 0.35mg HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate with ab178869 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab178869 at 1/1000 dilution (0.357 μg/ml). VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate (10ug).

Lane 2: ab178869 IP in HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate.

Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab178869 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 24 seconds.

Observed band: 76 kDa.

Lysates were made freshly and used in IP test immediately to minimize protein degradation.
All lanes: Western blot - Anti-Oct-1 antibody [EPR16570] (ab178869) at 1/30 dilution
Secondary
All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Predicted band size: 76 kDa
Observed band size: 76 kDa
Exposure time: 24s
Western blot - Anti-Oct-1 antibody [EPR16570] (ab178869)
Oct-1 Western blot staining using rabbit Anti-Oct-1 antibody
Exposure time: Lane 1-3: 15 seconds, Lane 4: 60 seconds
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Please note that the antibody detects extra bands on lysates from mouse and rat cell lines
All lanes: Western blot - Anti-Oct-1 antibody [EPR16570] (ab178869) at 1/1000 dilution
Lane 1: C6 (rat glioma) whole cell lysate at 10 µg
Lane 2: Raw 264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate at 10 µg
Lane 3: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg
Lane 4: NIH/3T3 (mouse embryo) whole cell lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 76 kDa
Observed band size: 95 kDa