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Mouse Monoclonal Oct-1 antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human POU2F1.


Images

Immunocytochemistry/ Immunofluorescence - Anti-Oct-1 antibody [POU5I097] (AB51363), expandable thumbnail
  • Flow Cytometry - Anti-Oct-1 antibody [POU5I097] (AB51363), expandable thumbnail
  • Western blot - Anti-Oct-1 antibody [POU5I097] (AB51363), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human POU2F1. The exact immunogen used to generate this antibody is proprietary information. Database link P14859

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBICC/IF
Human
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1 µg for 106 Cells
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR. (Microbial infection) In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.

Alternative names

OCT1, OTF1, POU2F1, NF-A1, Octamer-binding protein 1, Octamer-binding transcription factor 1, Oct-1, OTF-1

Recommended products

Mouse Monoclonal Oct-1 antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human POU2F1.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human POU2F1. The exact immunogen used to generate this antibody is proprietary information. Database link P14859
Clone number
POU5I097
Purification technique
Affinity purification Protein G
Concentration
Loading...
Purification notes

Filtered through a 0.22 µm membrane.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Oct-1 also known as POU domain class 2 transcription factor 1 (POU2F1) is a transcription factor characterized by its POU-specific domain. The molecular weight of Oct-1 is approximately 76 kDa. It is expressed widely across different tissues with high levels found in lymphoid organs and epithelial cells. It binds to octamer motifs in the promoters of target genes regulating their transcription. Oct-1 plays a role in the cell cycle development and immune response.

Biological function summary

Oct-1 participates in the regulation of gene expression by influencing RNA polymerase II. It is not part of a larger protein complex for its primary function but it does interact with other cofactors. Oct-1 is essential for maintaining the expression of housekeeping genes as well as for the induction of specific genes during external stimuli responses. By modulating cellular responses Oct-1 helps in the regulation of cells' growth differentiation and adaptation to stress.

Pathways

Oct-1 is involved in pathways related to stress responses and immune system function. It significantly influences the transcriptional activation of genes associated with the stress response pathway. Oct-1 associates with proteins like c-Myc and others within these pathways which contribute to the regulation of cell proliferation and apoptosis. Its role in these pathways highlights its involvement in cellular homeostasis and survival.

Associated diseases and disorders

Oct-1's dysregulation connects to the pathogenesis of various conditions including cancer and autoimmune diseases. Changes in Oct-1 activity have been observed in several cancers affecting tumorigenesis and progression. It interacts with proteins like Bcl-2 in the context of cancer impacting cell survival mechanisms. In autoimmune diseases such as lupus altered Oct-1 function can lead to inappropriate immune responses partly through interaction with transcription factors like NF-κB. Understanding the role of Oct-1 can help in developing targeted therapeutic strategies for these disorders.

Product promise

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3 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Oct-1 antibody [POU5I097] (ab51363), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Oct-1 antibody [POU5I097] (ab51363)

    ab51363 at 1:10 dilution staining HeLa cells; visualised with AlexaFluor®488 Goat Anti-mouse IgG at 1:200 dilution.

  • Flow Cytometry - Anti-Oct-1 antibody [POU5I097] (ab51363), expandable thumbnail

    Flow Cytometry - Anti-Oct-1 antibody [POU5I097] (ab51363)

    Overlay histogram showing Jurkat cells stained with ab51363 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51363, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1:500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Western blot - Anti-Oct-1 antibody [POU5I097] (ab51363), expandable thumbnail

    Western blot - Anti-Oct-1 antibody [POU5I097] (ab51363)

    All lanes: Western blot - Anti-Oct-1 antibody [POU5I097] (ab51363) at 1/50 dilution

    All lanes: HEK293 whole cell lysate at 50 µg

    Secondary

    All lanes: Anti mouse IgG antibody at 1/2500 dilution

    Predicted band size: 76 kDa

    Observed band size: 89 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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