Anti-Oct-1 antibody [RP23040247] - ChIP Grade

• ChIP

Supplier Data

ChIP - Anti-Oct-1 antibody [RP23040247] - ChIP Grade (AB308043)

Enrichment of endogenous Oct-1 protein at specific gene loci using ab308043 : Chromatin Immunoprecipitation (ChIP) was performed using ab308043 at 4 μg on sheared chromatin from 2 million HeLa cells. Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs over Promoters of HIST2H2BE, POLR2A (positive) and SAT2 satellite repeats (negative). Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

• WB

Supplier Data

Western blot - Anti-Oct-1 antibody [RP23040247] - ChIP Grade (AB308043)

Western blot analysis was performed on modified whole cell extracts (1% SDS) of HeLa (Lane 1), Hep G2 (Lane 2), A-431 (Lane 3), Ramos (Lane 4), HEK-293 (Lane 5). HCT 116 (Lane 6) and NTERA-2 (Lane 7). The blot was probed with ab308043 at 1 : 250 dilution and detected by chemiluminescence using a Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1 : 4000 dilution. A ~80 kDa band corresponding to Oct-1 was observed across the cell lines tested.

All lanes:

Western blot - Anti-Oct-1 antibody [RP23040247] - ChIP Grade (ab308043) at 1/250 dilution

Lane 1:

HeLa whole cell extract at 30 µg

Lane 2:

HepG2 whole cell extract at 30 µg

Lane 3:

A431 whole cell extract at 30 µg

Lane 4:

Ramos whole cell extract at 30 µg

Lane 6:

HCT 116 whole cell extract at 30 µg

Lane 7:

NTERA-2 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1/4000 dilution

Observed band size: 80 kDa

true

• WB

Supplier Data

Western blot - Anti-Oct-1 antibody [RP23040247] - ChIP Grade (AB308043)

Knockdown of Oct-1 was achieved by transfecting HeLa cells with Oct-1 specific siRNA. Western blot analysis was performed using modified whole cell extracts (1% SDS) from Oct-1 knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with ab308043 at 1/1000 dilution and a Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/4000 dilution.

All lanes:

Western blot - Anti-Oct-1 antibody [RP23040247] - ChIP Grade (ab308043) at 1/1000 dilution

Lane 1:

Untransfected HeLa cells

Lane 2:

non-specific scrambled siRNA transfected HeLa cells

Lane 3:

Oct-1 siRNA knockdown HeLa cells

Secondary

All lanes:

HRP-conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1/4000 dilution

Observed band size: 80 kDa

false