Anti-Oct-2 antibody [9A2]

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Oct-2 antibody [9A2] (AB243153)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Raji (Human Burkitt's lymphoma B lymphocyte) cell line labeling Oct-2 with ab243153 at 1.343μg/ml (red) compared withRat monclonal IgG (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rat IgG (Alexa Fluor^® 488) (ab150157)at 1/2000 dilution was used as the secondary antibody.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [9A2] (AB243153)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Oct-2 with ab243153 at 2.015μg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on human spleen (PMID : 11280769). The section was incubated with ab243153 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [9A2] (AB243153)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Oct-2 with ab243153 at 2.015μg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on human tonsil (PMID : 11280769). The section was incubated with ab243153 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [9A2] (AB243153)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Oct-2 with ab243153 at 2/015μg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on mouse spleen (PMID : 11280769). The section was incubated with ab243153 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [9A2] (AB243153)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Oct-2 with ab243153 at 2.015μg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on rat spleen (PMID : 11280769). The section was incubated with ab243153 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

• WB

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Western blot - Anti-Oct-2 antibody [9A2] (AB243153)

The molecular weight observed is consistent with what has been described in the literature (PMID : 3265124).

Exposure times : Lanes 1-3 : 3 seconds; Lane 4 : 3 minutes; Lane 5 : 15 seconds; Lane 6 : 44 seconds.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Oct-2 antibody [9A2] (ab243153) at 1/1000 dilution

Lane 1:

Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate at 20 µg

Lane 2:

Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate at 20 µg

Lane 3:

Daudi (human Burkitt's lymphoma lymphoblast), whole cell lysate at 20 µg

Lane 4:

A20 (mouse reticulum sarcoma B lymphocyte), whole cell lysate at 20 µg

Lane 5:

Human tonsil tissue lysate at 20 µg

Lane 6:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/10000 dilution

Predicted band size: 51 kDa

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