AB33919

Anti-Oct-2 antibody [EP543Y]

RabMAb
What is this?

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(3 Publications)

Rabbit Monoclonal Oct-2 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

OCT2, OTF2, POU2F2, Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2, Octamer-binding protein 2, Octamer-binding transcription factor 2, Oct-2, OTF-2

1 Images

Lab

Western blot - Anti-Oct-2 antibody [EP543Y] (AB33919)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Oct-2 antibody [EP543Y] (ab33919) at 1/10000 dilution

Lane 1:

Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 15 µg

Lane 2:

Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Carrier free

Anti-Oct-2 antibody [EP543Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP543Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody may detect the isoform 2.

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Reactivity data

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Product details

This product has switched from a hybridoma to recombinant production method on 13th September 2024.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Oct-2 also known as 9A2 or POU2F2 is a transcription factor with a molecular mass of approximately 50-55 kDa. It belongs to the POU family of transcription factors. Oct-2 is expressed widely in various tissues but shows higher expression levels in B lymphocytes and the central nervous system. This protein binds to the octamer DNA sequence motif which regulates the activity of several genes involved in immune function and neural development. Its expression pattern and DNA binding properties make Oct-2 essential in controlling transcriptional activation and repression.

Biological function summary

Oct-2 regulates the expression of target genes by binding to octamer motifs within promoters and enhancers. In the immune system Oct-2 plays a significant role in B cell differentiation and maturation. It acts as an essential transcriptional regulator of immunoglobulin gene expression. In neural tissues Oct-2 influences genes involved in neural differentiation and development. Oct-2 often does not act alone; it forms part of multi-protein complexes interacting with coactivators and corepressors to modulate gene expression tightly.

Pathways

Oct-2 participates in critical transcriptional regulatory networks. It is important in the immune response pathway controlling genes involved in B cell activation and antibody production. Oct-2 also interacts with Oct-1 another member of the POU family in controlling gene expression patterns related to adaptive immunity. Additionally Oct-2 influences pathways involved in neural development working alongside transcription factors like Pax-5 to regulate genes critical for the growth and differentiation of neuronal cells.

Oct-2 has been implicated in certain B cell-related malignancies and neurological disorders. Abnormal expression or mutation of Oct-2 can contribute to the development of lymphoma by altering the normal regulation of proliferation-related genes in B cells. Oct-2 also has connections to neurodevelopmental disorders where dysregulation of neural-specific genes controlled by Oct-2 may contribute to disease pathology. Understanding Oct-2's interaction with related proteins such as EBF1 in these disorders provides insights into the complex genetic regulation involved in these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3') (PubMed : 2904654, PubMed : 7859290). Regulates IL6 expression in B cells with POU2AF1 (By similarity). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression (PubMed : 2901913, PubMed : 2904654). Modulates transcription transactivation by NR3C1, AR and PGR (PubMed : 10480874).. Isoform 5. Activates the U2 small nuclear RNA (snRNA) promoter.

See full target information POU2F2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Carcinogenesis 39:191-201 PubMed29106514

2017

Functional role of PLCE1 intronic insertion variant associated with susceptibility to esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lixuan Wei,Mingming Shao,Yanjie Zhao,Jian Zheng,Jiahui Chu,Jiang Chang,Xinxin Cheng,Qionghua Cui,Linna Peng,Yingying Luo,Wenle Tan,Wen Tan,Dongxin Lin,Chen Wu

Biomedical reports 5:639-643 PubMed27882231

2016

Organic cation transporter 6 directly confers resistance to anticancer platinum drugs.

Applications

Species

Human

Tetsuya Oguri,Eiji Kunii,Satoshi Fukuda,Kazuki Sone,Takehiro Uemura,Osamu Takakuwa,Yoshihiro Kanemitsu,Hirotsugu Ohkubo,Masaya Takemura,Ken Maeno,Yutaka Ito,Akio Niimi

The Journal of biological chemistry 287:31983-93 PubMed22843692

2012

Prenyltransferases regulate CD20 protein levels and influence anti-CD20 monoclonal antibody-mediated activation of complement-dependent cytotoxicity.

Applications

ChIP

Species

Unspecified reactive species

Magdalena Winiarska,Dominika Nowis,Jacek Bil,Eliza Glodkowska-Mrowka,Angelika Muchowicz,Malgorzata Wanczyk,Kamil Bojarczuk,Michal Dwojak,Malgorzata Firczuk,Ewa Wilczek,Malgorzata Wachowska,Katarzyna Roszczenko,Marta Miaczynska,Justyna Chlebowska,Grzegorz Wladyslaw Basak,Jakub Golab

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Oct-2 antibody [EP543Y]

Western blot - Anti-Oct-2 antibody [EP543Y] (AB33919)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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