AB178679

Anti-Oct-2 antibody [EPR12482-106]

Name: Anti-Oct-2 antibody [EPR12482-106] (ab178679) | Abcam
Brand: Abcam Limited
SKU: AB178679
Price: 355 USD
Availability: InStock
Rating: 5 (3 reviews)

RabMAb
Recombinant
What is this?

(3 Reviews)

(4 Publications)

Rabbit Recombinant Monoclonal Oct-2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications.

View Alternative Names

OCT2, OTF2, POU2F2, Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2, Octamer-binding protein 2, Octamer-binding transcription factor 2, Oct-2, OTF-2

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [EPR12482-106] (AB178679)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [EPR12482-106] (AB178679)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Oct-2 with ab178679 at 1 : 100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [EPR12482-106] (AB178679)

Immunohistochemical analysis of paraffin-embedded Human DLBCL tissue labeling Oct-2 with ab178679 at 1 : 100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [EPR12482-106] (AB178679)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Oct-2 with ab178679 at 1 : 100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Oct-2 antibody [EPR12482-106] (AB178679)

All lanes:

Western blot - Anti-Oct-2 antibody [EPR12482-106] (ab178679) at 1/1000 dilution

Lane 1:

Ramos cell lysate at 10 µg

Lane 2:

Raji cell lysate at 10 µg

Lane 3:

Daudi cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Predicted band size: 51 kDa

false

Carrier free

Anti-Oct-2 antibody [EPR12482-106] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12482-106

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Oct-2 also known as 9A2 or POU2F2 is a transcription factor with a molecular mass of approximately 50-55 kDa. It belongs to the POU family of transcription factors. Oct-2 is expressed widely in various tissues but shows higher expression levels in B lymphocytes and the central nervous system. This protein binds to the octamer DNA sequence motif which regulates the activity of several genes involved in immune function and neural development. Its expression pattern and DNA binding properties make Oct-2 essential in controlling transcriptional activation and repression.

Biological function summary

Oct-2 regulates the expression of target genes by binding to octamer motifs within promoters and enhancers. In the immune system Oct-2 plays a significant role in B cell differentiation and maturation. It acts as an essential transcriptional regulator of immunoglobulin gene expression. In neural tissues Oct-2 influences genes involved in neural differentiation and development. Oct-2 often does not act alone; it forms part of multi-protein complexes interacting with coactivators and corepressors to modulate gene expression tightly.

Pathways

Oct-2 participates in critical transcriptional regulatory networks. It is important in the immune response pathway controlling genes involved in B cell activation and antibody production. Oct-2 also interacts with Oct-1 another member of the POU family in controlling gene expression patterns related to adaptive immunity. Additionally Oct-2 influences pathways involved in neural development working alongside transcription factors like Pax-5 to regulate genes critical for the growth and differentiation of neuronal cells.

Oct-2 has been implicated in certain B cell-related malignancies and neurological disorders. Abnormal expression or mutation of Oct-2 can contribute to the development of lymphoma by altering the normal regulation of proliferation-related genes in B cells. Oct-2 also has connections to neurodevelopmental disorders where dysregulation of neural-specific genes controlled by Oct-2 may contribute to disease pathology. Understanding Oct-2's interaction with related proteins such as EBF1 in these disorders provides insights into the complex genetic regulation involved in these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3') (PubMed : 2904654, PubMed : 7859290). Regulates IL6 expression in B cells with POU2AF1 (By similarity). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression (PubMed : 2901913, PubMed : 2904654). Modulates transcription transactivation by NR3C1, AR and PGR (PubMed : 10480874).. Isoform 5. Activates the U2 small nuclear RNA (snRNA) promoter.

See full target information POU2F2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 84:1149-1157.e7 PubMed38309274

2024

IκBζ is a dual-use coactivator of NF-κB and POU transcription factors.

Applications

Unspecified application

Species

Unspecified reactive species

Aktan Alpsoy,Xiaoli S Wu,Sujay Pal,Olaf Klingbeil,Pramod Kumar,Osama El Demerdash,Benan Nalbant,Christopher R Vakoc

Virchows Archiv : an international journal of pathology 483:81-86 PubMed36754897

2023

OCT2 expression in histiocytoses.

Applications

Unspecified application

Species

Unspecified reactive species

Irena Antonia Ungureanu,Fleur Cohen-Aubart,Sébastien Héritier,Sylvie Fraitag,Frédéric Charlotte,Hippolyte Lequain,Zofia Hélias-Rodzewicz,Julien Haroche,Jean Donadieu,Jean-François Emile

Frontiers in molecular neuroscience 12:263 PubMed31787878

2019

Pou2f2 Regulates the Distribution of Dorsal Interneurons in the Mouse Developing Spinal Cord.

Applications

Unspecified application

Species

Unspecified reactive species

Gauhar Masgutova,Audrey Harris,Benvenuto Jacob,Lynn M Corcoran,Frédéric Clotman

Frontiers in cellular neuroscience 13:184 PubMed31231191

2019

Onecut Factors and Pou2f2 Regulate the Distribution of V2 Interneurons in the Mouse Developing Spinal Cord.

Applications

Unspecified application

Species

Unspecified reactive species

Audrey Harris,Gauhar Masgutova,Amandine Collin,Mathilde Toch,Maria Hidalgo-Figueroa,Benvenuto Jacob,Lynn M Corcoran,Cédric Francius,Frédéric Clotman

View all publications

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Product promise

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For full details, please see our Terms & Conditions

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For licensing inquiries, please contact partnerships@abcam.com

Anti-Oct-2 antibody [EPR12482-106]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [EPR12482-106] (AB178679)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [EPR12482-106] (AB178679)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [EPR12482-106] (AB178679)

Western blot - Anti-Oct-2 antibody [EPR12482-106] (AB178679)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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