Anti-Oct-2 antibody [EPR12482] - BSA and Azide free

• WB

Unknown

Western blot - Anti-Oct-2 antibody [EPR12482] - BSA and Azide free (AB250114)

The observed bands are consistent with what are described in PMID : 26993806.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179808).

All lanes:

Western blot - Anti-Oct-2 antibody [EPR12482] (<a href='/en-us/products/primary-antibodies/oct-2-antibody-epr12482-ab179808'>ab179808</a>) at 1/5000 dilution

Lane 1:

Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Lane 2:

Mouse brain lysate at 20 µg

Lane 3:

Rat brain lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 51 kDa

Observed band size: 65 kDa

false

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Oct-2 antibody [EPR12482] - BSA and Azide free (AB250114)

Immunocytochemistry/ Immunofluorescence analysis of Ramos (Human Burkitt's lymphoma B lymphocyte) cells labeling Oct-2 with Purified ab179808 at 1/200 (2.7 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179808).

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Oct-2 antibody [EPR12482] - BSA and Azide free (AB250114)

Intracellular Flow Cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling Oct-2 with Purified ab179808 at 1/50 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179808).

• IP

Unknown

Immunoprecipitation - Anti-Oct-2 antibody [EPR12482] - BSA and Azide free (AB250114)

Purified ab179808 at 1/30 dilution (2ug) immunoprecipitating Oct-2 in Raji whole cell lysate.
Lane 1 (input) : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate (10μg)
Lane 2 (+) : ab179808 + Raji whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab179808 in Raji whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 58 kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179808).

All lanes:

Immunoprecipitation - Anti-Oct-2 antibody [EPR12482] (<a href='/en-us/products/primary-antibodies/oct-2-antibody-epr12482-ab179808'>ab179808</a>)

Predicted band size: 51 kDa

false