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AB236558

Anti-Oct-2 antibody [OCT2/2137]

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(1 Publication)

Mouse Monoclonal Oct-2 antibody. Suitable for IHC-P, WB, Protein Array and reacts with Human, Recombinant fragment, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human POU2F2 aa 100-300.

View Alternative Names

OCT2, OTF2, POU2F2, Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2, Octamer-binding protein 2, Octamer-binding transcription factor 2, Oct-2, OTF-2

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [OCT2/2137] (AB236558)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [OCT2/2137] (AB236558)

Formalin-fixed, paraffin-embedded human lymph node tissue stained for Oct-2 using ab236558 at 2 μg/ml in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [OCT2/2137] (AB236558)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [OCT2/2137] (AB236558)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for Oct-2 using ab236558 at 2 μg/ml in immunohistochemical analysis.

Protein Array - Anti-Oct-2 antibody [OCT2/2137] (AB236558)
  • Protein Array

Unknown

Protein Array - Anti-Oct-2 antibody [OCT2/2137] (AB236558)

ab236558 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Western blot - Anti-Oct-2 antibody [OCT2/2137] (AB236558)
  • WB

Supplier Data

Western blot - Anti-Oct-2 antibody [OCT2/2137] (AB236558)

All lanes:

Western blot - Anti-Oct-2 antibody [OCT2/2137] (ab236558) at 2 µg/mL

All lanes:

Ramos (human Burkitt's lymphoma cell line) cell lysate

Predicted band size: 51 kDa

false

Western blot - Anti-Oct-2 antibody [OCT2/2137] (AB236558)
  • WB

Supplier Data

Western blot - Anti-Oct-2 antibody [OCT2/2137] (AB236558)

All lanes:

Western blot - Anti-Oct-2 antibody [OCT2/2137] (ab236558) at 2 µg/mL

Lane 1:

Recombinant Oct-2 protein

Lane 2:

Daudi (human Burkitt's lymphoma cell line) cell lysate

Predicted band size: 51 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OCT2/2137

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

WB, Protein Array, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human POU2F2 aa 100-300. The exact immunogen used to generate this antibody is proprietary information.

P09086

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>Incubate with primary antibody for 30 minutes at RT.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "ProteinArray-species-checked": "guaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" }, "Recombinant fragment": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "ProteinArray-species-checked": "notRecommended", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" }, "Recombinant full length protein - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from bioreactor concentrate by Protein A/G.
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Oct-2 also known as 9A2 or POU2F2 is a transcription factor with a molecular mass of approximately 50-55 kDa. It belongs to the POU family of transcription factors. Oct-2 is expressed widely in various tissues but shows higher expression levels in B lymphocytes and the central nervous system. This protein binds to the octamer DNA sequence motif which regulates the activity of several genes involved in immune function and neural development. Its expression pattern and DNA binding properties make Oct-2 essential in controlling transcriptional activation and repression.
Biological function summary

Oct-2 regulates the expression of target genes by binding to octamer motifs within promoters and enhancers. In the immune system Oct-2 plays a significant role in B cell differentiation and maturation. It acts as an essential transcriptional regulator of immunoglobulin gene expression. In neural tissues Oct-2 influences genes involved in neural differentiation and development. Oct-2 often does not act alone; it forms part of multi-protein complexes interacting with coactivators and corepressors to modulate gene expression tightly.

Pathways

Oct-2 participates in critical transcriptional regulatory networks. It is important in the immune response pathway controlling genes involved in B cell activation and antibody production. Oct-2 also interacts with Oct-1 another member of the POU family in controlling gene expression patterns related to adaptive immunity. Additionally Oct-2 influences pathways involved in neural development working alongside transcription factors like Pax-5 to regulate genes critical for the growth and differentiation of neuronal cells.

Oct-2 has been implicated in certain B cell-related malignancies and neurological disorders. Abnormal expression or mutation of Oct-2 can contribute to the development of lymphoma by altering the normal regulation of proliferation-related genes in B cells. Oct-2 also has connections to neurodevelopmental disorders where dysregulation of neural-specific genes controlled by Oct-2 may contribute to disease pathology. Understanding Oct-2's interaction with related proteins such as EBF1 in these disorders provides insights into the complex genetic regulation involved in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3') (PubMed : 2904654, PubMed : 7859290). Regulates IL6 expression in B cells with POU2AF1 (By similarity). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression (PubMed : 2901913, PubMed : 2904654). Modulates transcription transactivation by NR3C1, AR and PGR (PubMed : 10480874).. Isoform 5. Activates the U2 small nuclear RNA (snRNA) promoter.
See full target information POU2F2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Medicine 100:e25028 PubMed33761659

2021

Clinicopathological significance of SOX4 and epithelial-mesenchymal transition markers in patients with laryngeal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Mingjie Zhang,Hui Li,Yuefeng Han,Mengjun Wang,Junjie Zhang,Shiyin Ma
View all publications
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Product promise

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