Rabbit Polyclonal Oct4 antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 14 publications. Immunogen corresponding to Recombinant Fragment Protein within Human POU5F1 aa 1-200.
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Publications
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- Frontiers in veterinary science 8:7061062021Generation of Trophoblast-Like Cells From Hypomethylated Porcine Adult Dermal Fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSharon Arcuri,Georgia Pennarossa,Fulvio Gandolfi,Tiziana A L BreviniPubMed 34350230
- Animals : an open access journal from MDPI 10:2020Long-term Preservation of Testicular Tissue Integrity and Viability Using Vitrification in the Endangered Black-Footed Ferret ().Applications:Unspecified applicationReactive species:Unspecified reactive speciesDavid Baruc Cruvinel Lima et. al.PubMed 33066219
- Oncology reports 44:577-5882020TNF‑α promotes the malignant transformation of intestinal stem cells through the NF‑κB and Wnt/β‑catenin signaling pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaopeng Zhao et. al.PubMed 32627006
- Cell biochemistry and function 38:1069-10782020LncRNA TUG1 facilitates proliferation, invasion and stemness of ovarian cancer cell via miR-186-5p/ZEB1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFu-Liang Zhan,Chun-Fang Chen,Mei-Zhen YaoPubMed 32390141
- Oncology letters 20:794-8022020SATB2 knockdown decreases hypoxia-induced autophagy and stemness in oral squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeijie Dong et. al.PubMed 32566006
- Experimental & molecular medicine 51:1-202019Silencing of long noncoding RNA HOXA11-AS inhibits the Wnt signaling pathway via the upregulation of HOXA11 and thereby inhibits the proliferation, invasion, and self-renewal of hepatocellular carcinoma stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun-Cheng Guo et. al.PubMed 31757938
- Theriogenology 142:184-1952019Key characteristics of the ovary and uterus for reproduction with particular reference to poly ovulation in the plains viscacha (Lagostomus maximus, chinchillidae).Applications:Unspecified applicationReactive species:Unspecified reactive speciesMirta Alicia Flamini,Rodrigo S N Barreto,Gustavo S S Matias,Alexander Birbrair,Tais Harumi de Castro Sasahara,Claudio Gustavo Barbeito,Maria Angelica MiglinoPubMed 31604179
- Cancer management and research 11:5075-50892019HMGB3 silence inhibits breast cancer cell proliferation and tumor growth by interacting with hypoxia-inducible factor 1α.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Gu et. al.PubMed 31213919
- PloS one 13:e02073172018Influence of warming and reanimation conditions on seminiferous tubule morphology, mitochondrial activity, and cell composition of vitrified testicular tissues in the domestic cat model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDavid Baruc Cruvinel Lima et. al.PubMed 30408126
- Cell death & disease 8:e29322017An autocrine inflammatory forward-feedback loop after chemotherapy withdrawal facilitates the repopulation of drug-resistant breast cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDeyong Jia et. al.PubMed 28703802
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human POU5F1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q01860
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Reactivity data
Select an application| Flow Cyt | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Monkey | Predicted | Predicted | Predicted |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/200 | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey | Dilution info - | Notes - |
Associated Products
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Target data
Function
The protein expressed by the gene POU5F1 is a transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and forms a trimeric complex with SOX2 or SOX15 on DNA. It controls the expression of several genes involved in embryonic development, including YES1, FGF4, UTF1, and ZFP206. This protein is critical for early embryogenesis and for maintaining embryonic stem cell pluripotency. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
OCT3, OCT4, OTF3, POU5F1, Octamer-binding protein 3, Octamer-binding protein 4, Octamer-binding transcription factor 3, Oct-3, Oct-4, OTF-3
Recommended products
Rabbit Polyclonal Oct4 antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 14 publications. Immunogen corresponding to Recombinant Fragment Protein within Human POU5F1 aa 1-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human POU5F1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q01860
- Purification technique
- Affinity purification Protein A
- Concentration
- Purification notes
Affinity Purified
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Oct4 also known as POU5F1 Oct 4 protein and hecho en Oct 4 is a transcription factor important for maintaining pluripotency in stem cells. Oct4 protein has a molecular weight of approximately 40-45 kDa. Stem and germ cells express Oct4 where it regulates gene expression essential for cell differentiation and self-renewal. In immunohistochemistry (IHC) studies scientists often use Oct4 staining to identify stem cell presence and behavior.
- Biological function summary
Oct4 acts in concert with other pluripotency factors like SOX2 and NANOG to form a regulatory network essential for maintaining stem cell identity. As part of this network the Oct4 transcription factor orchestrates the expression of genes that prevent differentiation and maintain the stem cell’s undifferentiated state. The protein directly activates the transcription of target genes involved in maintaining the pluripotent state.
- Pathways
Oct4 plays an important role in the maintenance of pluripotency and self-renewal in the embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) pathways. Oct4 partners with proteins such as SOX2 and KLF4 in these pathways to activate and repress a common set of downstream genes which control differentiation and proliferation. Oct4 ensures the blocking of differentiation signals while promoting self-renewal in pluripotent cells.
- Associated diseases and disorders
Aberrant Oct4 expression often links to oncogenesis and tumor progression particularly in testicular germ cell tumors and certain types of cancers. Oct4 overexpression may interact with c-MYC a protein linked to cancer cell proliferation advancing tumorigenesis. Additionally researchers investigate Oct4 as a diagnostic marker for cancer and its potential as a therapeutic target to inhibit cancer cell growth.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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7 product images
Western blot - Anti-Oct4 antibody (ab137427)
12% SDS PAGE
All lanes: Western blot - Anti-Oct4 antibody (ab137427) at 1/5000 dilution
All lanes: Mouse Embryonic Stem Cell whole cell lysate at 20 µg
Predicted band size: 38 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody (ab137427)
Immunofluorescent analysis of paraformaldehyde-fixed Human Embryonic Stem cells labelling Oct4 with ab137427 at 1:400 dilution. Lower image shows cells co-stained with Hoechst 33342.
Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody (ab137427)
Immunofluorescent analysis of paraformaldehyde-fixed Human embryonic stem cells labelling Oct4 with ab137427 at 1:200 dilution.
Flow Cytometry - Anti-Oct4 antibody (ab137427)
Flow cytometric analysis of Human Embryonic Stem cells labelling Oct4 with ab137427 at 1:50 dilution (green) or rabbit IgG (black).
Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody (ab137427)
Immunofluorescent analysis of paraformaldehyde-fixed Human embryonic stem cells labelling Oct4 with ab137427 at 1:100 dilution. Lower image shows cells co-stained with DAPI.
Western blot - Anti-Oct4 antibody (ab137427)
10% SDS PAGE
All lanes: Western blot - Anti-Oct4 antibody (ab137427) at 1/1000 dilution
All lanes: HeLa nuclear cell lysate at 20 µg
Predicted band size: 38 kDa
Western blot - Anti-Oct4 antibody (ab137427)
10% SDS PAGE
All lanes: Western blot - Anti-Oct4 antibody (ab137427) at 1/1000 dilution
All lanes: Human Embryonic Stem Cell whole cell lysate at 20 µg
Predicted band size: 193 kDa, 22 kDa, 38 kDa, 44 kDa, 50 kDa, 53 kDa, 54 kDa
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