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AB137427

Anti-Oct4 antibody

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(16 Publications)

Rabbit Polyclonal Oct4 antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 16 publications. Immunogen corresponding to Recombinant Fragment Protein within Human POU5F1 aa 1-200.

View Alternative Names

OCT3, OCT4, OTF3, POU5F1, Octamer-binding protein 3, Octamer-binding protein 4, Octamer-binding transcription factor 3, Oct-3, Oct-4, OTF-3

7 Images
Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody (AB137427)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody (AB137427)

Immunofluorescent analysis of paraformaldehyde-fixed Human embryonic stem cells labelling Oct4 with ab137427 at 1 : 200 dilution.

Flow Cytometry - Anti-Oct4 antibody (AB137427)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-Oct4 antibody (AB137427)

Flow cytometric analysis of Human Embryonic Stem cells labelling Oct4 with ab137427 at 1 : 50 dilution (green) or rabbit IgG (black).

Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody (AB137427)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody (AB137427)

Immunofluorescent analysis of paraformaldehyde-fixed Human Embryonic Stem cells labelling Oct4 with ab137427 at 1 : 400 dilution. Lower image shows cells co-stained with Hoechst 33342.

Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody (AB137427)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody (AB137427)

Immunofluorescent analysis of paraformaldehyde-fixed Human embryonic stem cells labelling Oct4 with ab137427 at 1 : 100 dilution. Lower image shows cells co-stained with DAPI.

Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody (AB137427)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody (AB137427)

Immunofluorescence analysis of NT2D1 cells fixed in 4% paraformaldehyde at RT for 15 min labelling Oct4 protein using ab137427 at a 1/500 dilution (Green). phalloidin, a cytoskeleton marker was stained at a 1/200 dilution (Red). Scale bar= 10µm.

Western blot - Anti-Oct4 antibody (AB137427)
  • WB

Supplier Data

Western blot - Anti-Oct4 antibody (AB137427)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Oct4 antibody (ab137427) at 1/5000 dilution

All lanes:

Human ESC tissue extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 38 kDa

false

Western blot - Anti-Oct4 antibody (AB137427)
  • WB

Supplier Data

Western blot - Anti-Oct4 antibody (AB137427)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Oct4 antibody (ab137427) at 1/10000 dilution

Lane 1:

Non-transfected NT2D1 whole cell extract at 30 µg

Lane 2:

shRNA transfected NT2D1 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 38 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, Flow Cyt, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human POU5F1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q01860

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50 - 1/200", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Monkey": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Affinity Purified
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Oct4 also known as POU5F1 Oct 4 protein and hecho en Oct 4 is a transcription factor important for maintaining pluripotency in stem cells. Oct4 protein has a molecular weight of approximately 40-45 kDa. Stem and germ cells express Oct4 where it regulates gene expression essential for cell differentiation and self-renewal. In immunohistochemistry (IHC) studies scientists often use Oct4 staining to identify stem cell presence and behavior.
Biological function summary

Oct4 acts in concert with other pluripotency factors like SOX2 and NANOG to form a regulatory network essential for maintaining stem cell identity. As part of this network the Oct4 transcription factor orchestrates the expression of genes that prevent differentiation and maintain the stem cell’s undifferentiated state. The protein directly activates the transcription of target genes involved in maintaining the pluripotent state.

Pathways

Oct4 plays an important role in the maintenance of pluripotency and self-renewal in the embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) pathways. Oct4 partners with proteins such as SOX2 and KLF4 in these pathways to activate and repress a common set of downstream genes which control differentiation and proliferation. Oct4 ensures the blocking of differentiation signals while promoting self-renewal in pluripotent cells.

Aberrant Oct4 expression often links to oncogenesis and tumor progression particularly in testicular germ cell tumors and certain types of cancers. Oct4 overexpression may interact with c-MYC a protein linked to cancer cell proliferation advancing tumorigenesis. Additionally researchers investigate Oct4 as a diagnostic marker for cancer and its potential as a therapeutic target to inhibit cancer cell growth.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
See full target information POU5F1
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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

BioImpacts : BI 13:289-300 PubMed37645025

2023

Differentiation and molecular characterization of endothelial progenitor and vascular smooth muscle cells from induced pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammadreza Dastouri,Hilal Ozdag,Ahmet Ruchan Akar,Alp Can

Analytical cellular pathology (Amsterdam) 2022:5632164 PubMed36247876

2022

TAZ Regulates the Cisplatin Resistance of Epithelial Ovarian Cancer Cells via the ANGPTL4/SOX2 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Caihong Li,Qin Wang,Youzhen Luo,Juan Xiang

Frontiers in veterinary science 8:706106 PubMed34350230

2021

Generation of Trophoblast-Like Cells From Hypomethylated Porcine Adult Dermal Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Sharon Arcuri,Georgia Pennarossa,Fulvio Gandolfi,Tiziana A L Brevini

Animals : an open access journal from MDPI 10: PubMed33066219

2020

Long-term Preservation of Testicular Tissue Integrity and Viability Using Vitrification in the Endangered Black-Footed Ferret ().

Applications

Unspecified application

Species

Unspecified reactive species

David Baruc Cruvinel Lima,Lúcia Daniel Machado da Silva,Paul Marinari,Pierre Comizzoli

Oncology reports 44:577-588 PubMed32627006

2020

TNF‑α promotes the malignant transformation of intestinal stem cells through the NF‑κB and Wnt/β‑catenin signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaopeng Zhao,Lu Ma,Lu Dai,Di Zuo,Xin Li,Hongli Zhu,Fang Xu

Cell biochemistry and function 38:1069-1078 PubMed32390141

2020

LncRNA TUG1 facilitates proliferation, invasion and stemness of ovarian cancer cell via miR-186-5p/ZEB1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Fu-Liang Zhan,Chun-Fang Chen,Mei-Zhen Yao

Oncology letters 20:794-802 PubMed32566006

2020

SATB2 knockdown decreases hypoxia-induced autophagy and stemness in oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Weijie Dong,Yawen Chen,Naiying Qian,Guoqi Sima,Jianming Zhang,Zhiqin Guo,Changlin Wang

Experimental & molecular medicine 51:1-20 PubMed31757938

2019

Silencing of long noncoding RNA HOXA11-AS inhibits the Wnt signaling pathway via the upregulation of HOXA11 and thereby inhibits the proliferation, invasion, and self-renewal of hepatocellular carcinoma stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Cheng Guo,Yi-Jun Yang,Jin-Fang Zheng,Jian-Quan Zhang,Min Guo,Xiang Yang,Xiang-Ling Jiang,Li Xiang,You Li,Huang Ping,Liu Zhuo

Theriogenology 142:184-195 PubMed31604179

2019

Key characteristics of the ovary and uterus for reproduction with particular reference to poly ovulation in the plains viscacha (Lagostomus maximus, chinchillidae).

Applications

Unspecified application

Species

Unspecified reactive species

Mirta Alicia Flamini,Rodrigo S N Barreto,Gustavo S S Matias,Alexander Birbrair,Tais Harumi de Castro Sasahara,Claudio Gustavo Barbeito,Maria Angelica Miglino

Cancer management and research 11:5075-5089 PubMed31213919

2019

HMGB3 silence inhibits breast cancer cell proliferation and tumor growth by interacting with hypoxia-inducible factor 1α.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Gu,Tao Xu,Qin-Hua Huang,Chu-Miao Zhang,Hai-Yan Chen
View all publications
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