Rabbit Polyclonal Pou5f1 antibody. Suitable for WB and reacts with Rat samples. Cited in 219 publications.
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- In vitro cellular & developmental biology. Animal 59:514-5272023Equine induced pluripotent stem cells are responsive to inflammatory cytokines before and after differentiation into musculoskeletal cell types.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEsther Palomino Lago,Elizabeth R Jelbert,Arabella Baird,Pak Y Lam,Deborah J GuestPubMed 37582999
- Cell journal 25:317-3262023Intravenous Transplantation of Adipose-Derived Mesenchymal Stem Cells Promoted The Production of Dopaminergic Neurons and Improved Spatial Memory in A Rat Model of Parkinson's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHelia Hamedi,S Hakiba Ghorbanian,Leila Mirzaeian,Kataneh Abrari,Paul Mozdziak,Mohammad Taghi GhorbanianPubMed 37300293
- Frontiers in immunology 14:11859212023Immunity and reproduction protective effects of Chitosan Oligosaccharides in Cyclophosphamide/Busulfan-induced premature ovarian failure model mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoyan Li,Haifeng Ye,Tie Su,Chuan Hu,Yaoqi Huang,Xinxin Fu,Zhisheng Zhong,Xuelian Du,Yuehui ZhengPubMed 37228612
- Journal of ovarian research 16:762023Chito-oligosaccharides and macrophages have synergistic effects on improving ovarian stem cells function by regulating inflammatory factors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesK Zheng,Wenli Hong,Haifeng Ye,Ziqiong Zhou,Shuyi Ling,Yuan Li,Yuqing Dai,Zhisheng Zhong,Ziwei Yang,Yuehui ZhengPubMed 37060101
- Animals : an open access journal from MDPI 13:2023Allogenic Synovia-Derived Mesenchymal Stem Cells for Treatment of Equine Tendinopathies and Desmopathies-Proof of Concept.Applications:Unspecified applicationReactive species:Unspecified reactive speciesInês Leal Reis,Bruna Lopes,Patrícia Sousa,Ana Catarina Sousa,Mariana Branquinho,Ana Rita Caseiro,Sílvia Santos Pedrosa,Alexandra Rêma,Cláudia Oliveira,Beatriz Porto,Luís Atayde,Irina Amorim,Rui Alvites,Jorge Miguel Santos,Ana Colette MaurícioPubMed 37106875
- Aging cell 22:e138252023Transcriptional activation of endogenous Oct4 via the CRISPR/dCas9 activator ameliorates Hutchinson-Gilford progeria syndrome in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunyeop Kim,Yerim Hwang,Sumin Kim,Yujung Chang,Yunkyung Kim,Youngeun Kwon,Jongpil KimPubMed 36964992
- IBRO neuroscience reports 14:235-2432023Neural fate commitment of rat full-term amniotic fluid stem cells three-dimensional embryoid bodies and neurospheres formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNuratiqah Azmi,Siti Sarah Mustaffa Al Bakri,Winnie Khor,Siti Nurusaadah Hamzah,Nurfarhana Ferdaos,King-Hwa Ling,Norshariza NordinPubMed 37388489
- Cellular and molecular gastroenterology and hepatology 15:741-7642022Dysfunctional Extracellular Matrix Remodeling Supports Perianal Fistulizing Crohn's Disease by a Mechanoregulated Activation of the Epithelial-to-Mesenchymal Transition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGiulia Rizzo,Federica Rubbino,Sudharshan Elangovan,Giusy Sammarco,Sara Lovisa,Silvia Restelli,Samuel Elias Pineda Chavez,Luca Massimino,Luigi Lamparelli,Marianna Paulis,Annalisa Maroli,Giulia Roda,Mohammad Shalaby,Michele Carvello,Caterina Foppa,Sheona P Drummond,Paola Spaggiari,Federica Ungaro,Antonino Spinelli,Alberto Malesci,Alessandro Repici,Anthony J Day,Alessandro Armuzzi,Silvio Danese,Stefania VetranoPubMed 36521659
- Cells 11:2022Long-Term Characteristics of Human-Derived Biliary Organoids under a Single Continuous Culture Condition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRanan G Aktas,Michael Karski,Biju Issac,Liang Sun,Shira Rockowitz,Piotr Sliz,Khashayar VakiliPubMed 36497057
- Stem cells international 2022:74943542022YTHDC1 Promotes Stemness Maintenance and Malignant Progression in Head and Neck Squamous Cell Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunquan Weng,Haidong Fan,Huijuan Liu,Su Tang,Yuyan ZhengPubMed 36411870
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 69% PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | |
|---|---|
| Human | Predicted |
| Mouse | Predicted |
| Rat | Tested |
| Chimpanzee | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1-2 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Chimpanzee | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Alternative names
MGC22487, OTF 4, OTF-3, Oct-03, Oct-04, Octamer binding transcription factor 4, Octamer-binding protein 3, Octamer-binding protein 4, Octamer-binding transcription factor 3, PO5F1_HUMAN, POU class 5 homeobox 1, POU domain class 5 transcription factor 1, POU domain transcription factor OCT4, POU-type homeodomain-containing DNA-binding protein, POU5F1
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Rabbit Polyclonal Pou5f1 antibody. Suitable for WB and reacts with Rat samples. Cited in 219 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 69% PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Protein A
- Specificity
The antibody reacts with 45 kDa Oct4 (also designated Oct3A) and in a less extent 33 kDa Oct3B.
- Concentration
- Purification notes
Affinity Purified
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is manufactured by BioVision, an Abcam company and was previously called 3576 Oct-4 Antibody. 3576-100 is the same size as the 100 µg size of ab18976.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Oct4 also known as POU5F1 Oct 4 protein and hecho en Oct 4 is a transcription factor important for maintaining pluripotency in stem cells. Oct4 protein has a molecular weight of approximately 40-45 kDa. Stem and germ cells express Oct4 where it regulates gene expression essential for cell differentiation and self-renewal. In immunohistochemistry (IHC) studies scientists often use Oct4 staining to identify stem cell presence and behavior.
- Biological function summary
Oct4 acts in concert with other pluripotency factors like SOX2 and NANOG to form a regulatory network essential for maintaining stem cell identity. As part of this network the Oct4 transcription factor orchestrates the expression of genes that prevent differentiation and maintain the stem cell’s undifferentiated state. The protein directly activates the transcription of target genes involved in maintaining the pluripotent state.
- Pathways
Oct4 plays an important role in the maintenance of pluripotency and self-renewal in the embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) pathways. Oct4 partners with proteins such as SOX2 and KLF4 in these pathways to activate and repress a common set of downstream genes which control differentiation and proliferation. Oct4 ensures the blocking of differentiation signals while promoting self-renewal in pluripotent cells.
- Associated diseases and disorders
Aberrant Oct4 expression often links to oncogenesis and tumor progression particularly in testicular germ cell tumors and certain types of cancers. Oct4 overexpression may interact with c-MYC a protein linked to cancer cell proliferation advancing tumorigenesis. Additionally researchers investigate Oct4 as a diagnostic marker for cancer and its potential as a therapeutic target to inhibit cancer cell growth.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-Oct4 antibody (ab18976)
The antibody reacts with 45 kDa Oct4 (also designated Oct-3A) and in a less extent 33 kDa Oct-3B from human, mouse, and rat samples.
All lanes: Western blot - Anti-Oct4 antibody (ab18976) at 0.5 µg/mL
All lanes: Lysates prepared from rat kidney tissue.
Predicted band size: 38 kDa
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